Reduced genome E. coli

ABSTRACT

Reduced genome strains of  E. coli  MG1655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.

FIELD OF THE INVENTION

The present invention relates to strains of microorganisms and processes involving these microorganisms. More specifically, the present invention relates to genetically modified strains of microorganisms and the use thereof for the production of commercial products, such as recombinant proteins, nucleic acids, such as DNA, and amino acids. The present invention also relates to methods of preparing strains of microorganisms for such uses.

BACKGROUND OF THE INVENTION

Bacteria have been used to produce a wide range of commercial products. For example, many Streptomyces strains and Bacillus strains have been used to produce antibiotics; Pseudomanas denitrificans and many Propionibacterium strains have been used to produce vitamin B12; some other bacteria have been used to produce vitamin Riboflavin; Brevibacterium flavum and Corynebacterium glutamicum have been used to produce lysine and glutamic acid, respectively, as food additives; other bacteria have been used to produce other amino acids used as food additives; Alcaligenes eutrophas has been used to produce biodegradable microbial plastics; and many Acetobacter and Gluconobacter strains have been used to produce vinegar. More recently, it has become common for bacteria, such as Escherichia coli (E. coli), to be genetically engineered and used as host cells for the production of biological reagents, such as proteins and nucleic acids, in laboratory as well as industrial settings. The pharmaceutical industry supports several examples of successful products which are human proteins which are manufactured in E. coli cultures cultivated in a fermenter.

It is not an uncommon occurrence for normal bacterial proteins to adversely affect the production or the purification of a desired protein product from an engineered bacteria. For example, when E. coli bacteria are used as host cells to generate a large quantity of a desired product encoded by a gene that is introduced into the host cells by a plasmid, certain normal E. coli gene products can interfere with the introduction and maintenance of plasmid DNA. More significantly, because of the economies of bacterial culture in making proteins in bacteria, often the cost of purification of a recombinant protein can be more than the cost of production, and some of the natural proteins produced by the bacterial host are sensitive to purification procedures. Further, many bacterial strains produce toxins that must be purified away from the target protein being produced and some strains can produce, by coincidence, native proteins that are close in size to the target protein, thereby making size separation ineffective for the purification process.

The genome of a bacteria used in a fermenter to produce a recombinant protein includes many unnecessary genes. A bacteria living in a natural environment has many condition responsive genes to provide mechanisms for surviving difficult environmental conditions of temperature, stress or lack of food source. Bacteria living in a fermentation tank do not have these problems and hence do not require these condition responsive genes. The bacterial host spends metabolic energy each multiplication cycle replicating these genes. Thus the unnecessary genes and the unneeded proteins, produced by a bacterial host used for production of recombinant protein, result in a lack of efficiencies in the system that could be improved upon.

It is not terribly difficult to make deletions in the genome of a microorganism. One can perform random deletion studies in organisms by simply deleting genomic regions to study what traits of the organism are lost by the deleted genes. It is more difficult, however, to make targeted deletions of specific regions of genomic DNA and more difficult still if one of the objectives of the method is to leave no inserted DNA or mutations, here termed a “scar,” in the genome of the organism after the deletion. If regions of inserted DNA or mutations, i.e. scars, are left behind after a genomic deletion procedure, those regions can be the locations for unwanted recombination events that could excise from the genome regions that are desirable or engender genome rearrangements. In building a series of multiple deletions, scars left behind in previous steps could become artifactual targets for succeeding steps of deletion. This is especially so when the method is used repeatedly to generate a series of deletions from the genome. In other words, the organism becomes by the deletion process genetically unstable if inserted DNA or mutations are left behind.

BRIEF DESCRIPTION OF SEQUENCE LISTING, TABLES AND COMPUTER PROGRAM LISTING

A sequence listing is attached to the present application in a file named SEQ.txt (5740 KB, 19 May 5), and is hereby incorporated by reference herein.

SUMMARY OF THE INVENTION

In one embodiment of the invention, a reduced genome strain is provided, which may be lacking the nucleic acid regions shown on Table 2 or a region with a sequence substantially identical theretoand the following ranges of nucleic acid sequences with reference to SEQ ID NO: 1: 167484-169727, 169778-170575, 170575-171465 and 171462-173444 or a sequence substantially identical thereto. The genome of the strain may be further lacking the following range of nucleic acid sequence with reference to SEQ ID NO: 1: 3088369-3089076 or a sequence substantially identical thereto. The genome of the strain may also be further lacking the following range of nucleic acid sequence with reference to SEQ ID NO: 1: 2820730-2821791 or a sequence substantially identical thereto.

In another embodiment, the invention provides a strain of E. coli lacking one or more of the nucleic acid regions shown on any of Tables 1-20, or sequences substantially identical thereto.

In still another embodiment, the invention provides a strain of E. coli lacking one or more of the nucleic acid regions shown on any of Tables 1-20, or sequences substantially identical thereto.

The parent of the reduced genome strain may be E. coli. The parent of the reduced genome strain may also be E. coli MG1655. The genomic sequence of the parent of the reduced genome strain may be the sequence set forth in SEQ ID NO: 1 or a sequence substantially identical thereto.

The strain may comprise a heterologous nucleic acid. The genome of the strain may comprise the heterologous nucleic acid. The heterologous nucleic acid may be a vector, such as a plasmid. The plasmid may also comprise a heterologous nucleic acid. The heterologous nucleic acid may be operatively linked to an expression control sequence. The strain may be used to produce a polypeptide by expressing a polypeptide encoded by the heterologous nucleic acid. The heterologous nucleic acid may be introduced into the strain by transforming the strain with the heterologous nucleic acid. The heterologous nucleic acid may be amplified in the strain.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is an illustration of the original release of the E. coli K-12 genome (strain MG1655-annotation version m54), which natively comprises 4,639,221 nucleotides or base pairs. The inner ring of FIG. 1 shows the scale of the base pair positions without deletions (see also Blattner et al., infra). The next ring progressively outward shows regions of the K-12 genome that are missing or highly altered in a related strain O157:H7, and which are thus potentially deletable from the K-12 genome. The next ring outward shows the positions of the IS elements, both complete and partial, in the native genome. The next ring moving outward shows the positions of the RHS elements A to E and flagellar and restriction regions. The outermost ring shows the location of the deletions actually made to the genome, as described in U.S. Patent Publication No. 20030138937 and International Patent Publication No. WO 2003/070880, the contents of which are incorporated herein by reference, which delete up to about 14 percent of the base pairs in the original K-12 MG1655 genome.

FIG. 2 shows a comparison of the growth rate of MG1655 and MDS42 in MOPS minimal medium at 37° C.

FIG. 3 shows a comparison of the expression of chloramphenicol acetyl transferase (CAT) in MDS42 (top) and MG1655 (bottom).

FIG. 4 shows a comparison of the mutation rates in MDS41 and MG1655 based on cyca mutations causing D-cycloserine resistance.

FIG. 5 shows the PCR detection of IS elements in various DNA preparations. Each panel is loaded in the same order: 1 kb+ marker, IS, IS2, IS3, IS5, IS10, IS186, 1 kb+ marker, for (1) Positive Control, (2) Negative Control, (3) pBR322 from Invitrogen, and (4) pBR322 produced in MDS42.

FIG. 6 shows the restriction pattern of pCTXVP60 propagated in various strains: (M) molecular weight marker, 1 kbp ladder; (1) MDS41, no insertion; (2) MDS42, no insertion; (3) DH10B, IS10 insertion; (4) DH10B, IS10 insertion/deletion; (5) C600, IS5 insertion; (6) C600, IS1 insertion; and (7) C600, IS1 insertion. Below the gel, the relative positions of the IS insertions in the CTXVP60 reading frame are diagrammed.

DETAILED DESCRIPTION OF THE INVENTION

While the present invention is capable of being embodied in various forms, the description below of several embodiments is made with the understanding that the present disclosure is to be considered as an exemplification of the invention, and is not intended to limit the invention to the specific embodiments illustrated. Headings are provided for convenience only and are not to be construed to limit the invention in any way. Embodiments illustrated under any heading may be combined with embodiments illustrated under any other heading.

The use of numerical values in the various ranges specified in this application, unless expressly indicated otherwise, are stated as approximations as though the minimum and maximum values within the stated ranges were both preceded by the word “about.” In this manner, slight variations above and below the stated ranges can be used to achieve substantially the same results as values within the ranges. As used herein, the terms “about” and “approximately” when referring to a numerical value shall have their plain and ordinary meanings to one skilled in the pertinent at issue. Also, the disclosure of ranges is intended as a continuous range including every value between the minimum and maximum values recited as well as any ranges that can be formable thereby.

It is also to be understood that any ranges, ratios and ranges of ratios that can be formed by any of the numbers or data present herein represent further embodiments of the present invention. This includes ranges that can be formed that do or do not include a finite upper and/or lower boundary. Accordingly, the skilled person will appreciate that many such ratios, ranges, and ranges of ratios can be unambiguously derived from the data and numbers presented herein and all represent embodiments of the present invention.

Before the present compounds, products and compositions and methods are disclosed and described, it is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting. It must be noted that, as used in the specification and the appended claims, the singular forms “a,” “an” and “the” include plural referents unless the context clearly dictates otherwise.

1. DEFINITIONS

“Base pair” used herein may refer to the hydrogen bonded nucleotides of, for example, adenine (A) with thymine (T), or of cytosine (C) with guanine (G) in a double-stranded DNA molecule. In RNA, uracil (U) is substituted for thymine. Base pair may also be used as a unit of measure for DNA length.

“Clone” used in reference to an insert sequence and a vector may mean ligation of the insert sequence into the vector or its introduction by recombination either homologous, site specific or illegitimate as the case may be. When used in reference to an insert sequence, a vector, and a host cell, the term may mean to make copies of a given insert sequence. The term may also refer to a host cell carrying a cloned insert sequence, or to the cloned insert sequence itself.

“Complement,” “complementary” or “complementarity” used herein may mean Watson-Crick or Hoogsteen base pairing between nucleotides or nucleotide analogs of nucleic acid molecules. For example, the sequence 5′-A-G-T-3′ is complementary to the sequence 3′-T-C-A-5′. Complementarity may be “partial,” in which only some of the nucleotides are matched according to the base pairing rules. Or, there may be “complete” or “total” complementarity between the nucleic acids. The degree of complementarity between nucleic acid strands may have effects on the efficiency and strength of hybridization between nucleic acid strands.

“Encoding” or “coding” used herein when referring to a nucleic acid may mean a sequence of nucleotides, which upon transcription into RNA and subsequent translation into protein, would lead to the synthesis of a given protein, peptide or amino acid sequence. Such transcription and translation may actually occur in vitro or in vivo, or may be strictly theoretical based on the standard genetic code.

“Enzyme” used herein may mean a protein which acts as a catalyst to induce a chemical change in other compounds, thereby producing one or more products from one or more substrates. Enzymes are referred to herein using standard nomenclature or by their EC number, as recommended by the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology as of Mar. 11, 2004.

“Expression control sequence” used herein may mean a promoter or array of transcription factor binding sites that direct transcription of a nucleic acid operatively linked thereto.

“Free end” used in reference to a double-stranded nucleic acid may mean a linear nucleic acid with blunt free ends or sticky free ends, or a combination thereof.

“Gene” used herein may refer to a nucleic acid (e.g., DNA or RNA) that comprises a nucleic acid sequence encoding a polypeptide or precursor thereto. The polypeptide can be encoded by a full length coding sequence or by any portion of the coding sequence so long as the desired activity or functional properties (e.g., enzymatic activity, ligand binding, signal transduction, antigenicity etc.) of the full-length or fragment are retained. The term also encompasses the sequences located adjacent to the coding region on both the 5′ and 3′ ends that contribute to the gene being transcribed into a full-length mRNA. The term “gene” encompasses both cDNA and genomic forms of a gene. A genomic form or clone of a gene may contain the coding region interrupted with non-coding sequences termed (e.g., introns).

“Introduce” or “introduced” when used in reference to adding a nucleic acid to a strain, may mean that the nucleic acid may be integrated into the chromosome of the strain or contained on a vector, such as a plasmid, in the strain.

“Library” used herein may refer to a plurality of vectors each comprising an insert sequence or to a plurality of nucleotide fragments.

“Mutant” or “mutagenesis” used herein may mean any modification to the nucleic acid of a parent strain. Mutagenesis of nucleic acid may be of any type including, but not limited to, deletion, insertion, substitution, rearrangement, and suppressor and point mutations.

“Nucleic acid” used herein may mean any nucleic acid containing molecule including, but not limited to, DNA or RNA. The term encompasses sequences that include any base analogs of DNA and RNA including, but not limited to, 4-acetylcytosine, 8-hydroxy-N-6-methyladenosine, aziridinylcytosine, pseudoisocytosine, 5-(carboxyhydroxylmethyl)uracil, 5-fluorouracil, 5-bromouracil, 5-carboxymethylaminomethyl-2-thiouracil, 5 carboxymethylaminomethyluracil, dihydrouracil, inosine, N6-isopentenyladenine, 1-methyladenine, 1-methylpseudouracil, 1-methylguanine, 1-methylinosine, 2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine, 5-methylcytosine, N6-methyladenine, 7-methylguanine, 5-methylaminomethyluracils, 5-methoxyaminomethyl-2-thiouracil, ÿ-D-maninosylqueosine, 5′-methoxycarbonylmethyluracil, 5-methoxyuracil, 2-methylthio-N6-isopentenyladenine, uracil-5-oxyacetic acid methylester, uracil-5-oxyacetic acid, oxybutoxosine, pseudouracil, queosine, 2-thiocytosine, 5-methyl-2-thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil, N-uracil-5-oxyacetic acid methylester, uracil-5-oxyacetic acid, pseudouracil, queosine, 2-thiocytosine, and 2,6-diaminopurine.

“Nucleotide” used herein may refer to a monomeric unit of nucleic acid (e.g. DNA or RNA) consisting of a pentose sugar moiety, a phosphate group, and a nitrogenous heterocyclic base. The base may be linked to the sugar moiety via the glycosidic carbon (1′ carbon of the pentose). The combination of base and sugar may be called a nucleoside. When the nucleoside contains a phosphate group bonded to the 3′ or 5′ position of the pentose, it may be referred to as a nucleotide. A sequence of operatively linked nucleotides may be referred to as a “base sequence” or “nucleotide sequence” or “nucleic acid sequence,” and may be represented herein by a formula whose left to right orientation is in the conventional direction of 5′-terminus to 3′-terminus.

“Operably linked” used herein may refer to an expression control sequence and downstream polynucleotide, such that productive transcription of the polynucleotide is initiated at the expression control sequence.

“Overexpressing” used herein may mean that the total cellular activity of protein encoded by a gene is increased. The total cellular activity of a protein may be due to increased cellular amounts of a protein, or increased half-life of the protein. Total cellular amounts of a protein may be increased by methods including, but not limited to, amplification of the gene coding said protein or operatively linking a strong promoter to the gene coding said protein.

“Protein” used herein may mean a peptide, polypeptide and protein, whether native or recombinant, as well as fragments, derivatives, homologs, variants and fusions thereof.

“Region of comparison” used herein when referring to a genome may be 1×10⁷, 1.5×10⁷, 2×10⁷, 2.5×10⁷, 3×10⁷, 3.5×10⁶, 4×10⁷ or more nucleotides or base pairs, and when referring to a nucleic acid sequence may be 50, 100, 250, 500, 10³, 5×10³, 10⁴, 5×10⁴, 10⁵, 5×10⁵, 10⁶ or more nucleotides or more base pairs.

“Stringent hybridization conditions” used herein may mean conditions under which a first nucleic acid sequence will specifically or selectively hybridize to a second nucleic acid sequence, such as in a complex mixture of nucleic acids, but to no other sequences. Stringent conditions are sequence-dependent and will be different in different circumstances. Generally, stringent conditions are selected to be about 5-10° C. lower than the thermal melting point (Tm) for the specific sequence at a defined ionic strength pH. The Tm may be the temperature (under defined ionic strength, pH, and nucleic concentration) at which 50% of the first sequence hybridizes to the second sequence at equilibrium (as the second sequences are present in excess, at Tm, 50% of the first sequence are occupied at equilibrium). Stringent conditions may be those in which the salt concentration is less than about 1.0 M sodium ion, typically about 0.01-1.0 M sodium ion concentration (or other salts) at pH 7.0 to 8.3 and the temperature is at least about 30° C. for short nucleic acid sequences (e.g., about 10-50 nucleotides) and at least about 60° C. for long nucleic acid sequences (e.g., greater than about 50 nucleotides). Stringent conditions may also be achieved with the addition of destabilizing agents such as formamide. For selective or specific hybridization, a positive signal may be at least 2 to 10 times background hybridization. Exemplary stringent hybridization conditions include the following: 50% formamide, 5×SSC, and 1% SDS, incubating at 42° C., or, 5×SSC, 1% SDS, incubating at 65° C., with wash in 0.2×SSC, and 0.1% SDS at 65° C.

“Substantially complementary” used herein may mean that a first sequence is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 97%, 98% or 99% identical to the complement of a second sequence over a region of comparison or that the two sequences hybridize under stringent hybridization conditions.

“Substantially identical” used herein may mean that a first and second sequence are at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 97%, 98% or 99% identical or substantially complementary over a region of comparison. A reference sequence and a test sequence may be aligned, manually or by a computer algorithm (e.g., GAP, BESTFIT, FASTA and TFAST), and the percentage identity calculated by dividing “the total number of identical residues” by “the total number of residues in the reference sequence” and then multiplying by 100.

2. REDUCED GENOME STRAINS

Bacteria in their natural environment are exposed to many conditions that are not normally experienced in standard industrial or laboratory growth, and thus carry a large number of condition-dependent, stress-induced genes or otherwise nonessential genes which may not be needed in industrial or laboratory use of the organisms. It was realized that much of the genetic information contained within the genome of a bacteria strain could be deleted without detrimental effect to use of bacteria cultures in processes of industrial or laboratory importance. It was also recognized that a bacterium with a reduced genome might be advantageous over native strains in many industrial and laboratory applications. For example, a bacterium with a reduced genome is at least somewhat less metabolically demanding and thus can produce a desired product more efficiently. In addition, a reduced genome can lead to fewer native products and lower levels of certain native proteins, allowing easier purification of a desired protein from the remaining bacterial proteins. Furthermore, some bacterial genetic sequences are associated with instabilities that can interfere with standard industrial or laboratory practices, and might entail costly and burdensome quality control procedures.

The reduced genome strain may have a genome that is at least two percent (2%), five percent (5%), seven percent (7%) to eight percent (8%) to fourteen percent (14%) to eighteen percent (18%) to twenty percent (20%), to forty percent (40%) to sixty percent (60%) smaller than the genome of its native parental strain. The percentage by which a genome has become smaller after a series of deletions is calculated by dividing “the total number of base pairs deleted after all of the deletions” by “the total number of base pairs in the genome of the parental strain before all of the deletions” and then multiplying by 100.

The reduced genome strain may also have a genome that has about 5% to about 10%, about 10% to about 20%, about 30% to about 40%, or about 60% of its protein encoding genes deleted. The percentage by which a genome has had protein encoding genes deleted is calculated by dividing “the total number of protein encoding genes deleted after all of the deletions” by “the total number of protein encoding genes in the genome of the parental strain before all of the deletions” and then multiplying by 100.

The genes and other nucleic acid sequences deleted from the genome may not adversely affect the rate of survival and proliferation of the reduced genome strain under specific growth conditions. Whether a level of adverse effect is acceptable depends on a specific application. For example, a 30% reduction in proliferation rate may be acceptable for one application but not another. In addition, adverse effect of deleting a nucleic acid sequence from the genome may be reduced by measures such as changing culture conditions. Such measures may turn an unacceptable adverse effect to an acceptable one. The proliferation rate may be increased or approximately the same as the parental strain. The proliferation rate may also be from about 5%, 10%, 15%, 20%, 30%, 40% to about 50% lower than that of the parental strain. The doubling time of the reduced genome strain may range from about thirty minutes to about three hours.

a. Parent

The parental strain may be any bacteria strain or other organism, as well as an intermediate strain from which the reduced genome strain is derived. Representative examples of parent strains include, but are not limited to, E. coli strains such as K-12 or B, or a strain with a genome sequence substantially identical thereto. The K-12 strain may be MG1655. The nucleotide sequence of the genome of the parental strain may be partially or completely known. The complete genomic sequence of several strains of E. coli and other commonly used laboratory microorganisms is known (see, e.g., Blattner et al, Science, 277:1453-74, 1997; GenBank Accession No. U00096; Perna et al., Nature, 409, 529-533, 2001; Hayashi et al, DNA Res., 8, 11-22, 2001; Welch et al., Proc. Natl. Acad. Sci., USA 99:17020-17024, 2002 and GenBank Accession No. AE014075, each of which is incorporated herein by reference.

The nucleic acid sequence of E. coli MG1655 (annotated version in56) is set forth in SEQ ID NO: 1 with a total size of 4,639,675 nucleotides or base pairs. The original release of the genomic sequence of E. coli MG1655 was annotated version m54 (4,639,221 nucleotides or base pairs). Each gene that was predicted to occur in the original release of the E. coli MG1655 genome was assigned a unique numeric identifier beginning with a lowercase “b”. The first predicted gene was thrL with b0001 the last gene was lasT with b4403, since the initial release several new genes have been found which were given unauthenticated b-numbers with a suffix of “a” or “0.1” by various groups for example yaaV. Some of these genes were accepted in the most recent annotation (version m56) and have had new b-numbers assigned starting from b4404. Several genes in the m54 version have since been shown to be merged together or split apart caused by sequencing errors these have all been reissued new b-numbers from b4460 to b4500 and the old b-number deaccessioned.

b. Genomic Deletions

The reduced genome strain may be lacking one or more of the nucleic acid regions set forth on Table 1 or sequences substantially identical thereto. Table 1 provides the following annotated features of the genome for E. coli MG1655 (annotated version m56) (SEQ ID NO: 1): Column 1 (Type) lists the type of annotated feature (coding sequence (CDS), prophage, rep_origin, repeat region, rRNA, tRNA, misc_RNA or misc_feature); Column 2 (S) lists the reference strand (+: forward, −: complementary); Column 3 (Left) lists the position of the left end of the feature; Column 4 (Right) lists the position of the right end of the feature; Column 5 (Name) lists the name of the feature; and Column 6 (B) lists the Blattner number or b-number of the feature. The reduced genome strain may be MDS41, MDS42, MDS43, MDS44, MDS45, MDS46, MDS47, MDS48, MDS49, MDS50, MDS51, MDS52, MDS53, MDS54, MDS55, MDS56, MDS57, MDS58, MDS59, MDS60 or a strain with a genome substantially identical thereto. The reduced genome strain may also be MDS42recA⁻ or a strain with a genome substantially identical thereto.

One type of nucleic acid sequence that may be deleted includes those that may adversely affect the stability of the organism or of the gene products of that organism. Such elements that give rise to instability include transposable elements, insertion sequences, and other “selfish DNA” elements that may play a role in genome instability. These elements, either intact or defective, may move from one point to another in the genome or other nucleic acid sequences in the cell, such as a vector. These elements may not be important for bacteria survival and growth in a cultured environment. Positions of the IS elements on a genome map of E. coli MG1655 (annotated version m54) are shown in FIG. 1 and Table 1 of U.S. Patent Publication No. 20030138937 and International Patent Publication No. WO 2003/070880, the contents of which are incorporated herein by reference. Other nucleic acid sequences associated with genomic instability may also be deleted.

Another type of nucleic acid sequence that may be deleted is the restriction modification system genes and other endogenous nucleases whose products may destroy foreign DNA. These genes may not be important for bacterial survival and growth in culture environments. These genes may also interfere with genetic engineering by destroying vectors introduced into a bacterium, Positions of restriction modification system genes on a genome map of E. Coli MG1655 (annotated version m54) are shown in FIG. 1 and Table 1 of U.S. Patent Publication No. 20030138937 and International Patent Publication No. WO 2003/070880, the contents of which are incorporated herein by reference. DNA methylase genes, including heterologous genes, may be added back to the deleted strain so as to optimize the strain for certain uses, for example, eukaryotic methylase genes.

Another type of nucleic acid sequence that may be deleted is the flagella gene family, which may provide motility for bacteria. In cultured environments, bacteria motility may not be important for cell survival and growth and the swimming action may be metabolically very expensive, consuming over 1% of the cellular energy to no benefit. Positions of flagella genes on a genome map of E. coli MG1655 (annotated version m54) are shown in FIG. 1 and Table 1 of U.S. Patent Publication No. 20030138937 and International Patent Publication No. WO 2003/070880, the contents of which are incorporated herein by reference.

Another type of nucleic acid sequence that may be deleted is Rhs elements. Rhs elements may share a 3.7 Kb Rhs core, which may be a large homologous repeated region (there may be 5 copies in E. coli K-12) that may provide a means for genome rearrangement via homologous recombination. Mhs elements may be accessory elements which largely evolved in some other background and spread to E. coli by horizontal exchange after divergence of E. coli as a species, Positions of Rhs elements on a genome map of E. coli MG1655 (annotated version m54) are shown in FIG. 1 and Table 1 of U.S. Patent Publication No. 20030138937 and International Patent Publication No. WO 2003/070880, the contents of which are incorporated herein by reference.

Another type of nucleic acid sequence that may be deleted is the non-transcribed regions, which may be less important for cell survival and proliferation. Another type of nucleic acid sequence that can be deleted is the hsd regions, which may encode the major restriction modification gene family. Positions of non-transcribed regions and hsd regions on a genome map of E. coli MG1655 (annotated version m54) are shown in FIG. 1 and Table 1 of U.S. Patent Publication No. 20030138937 and International Patent Publication No. WO 2003/070880, the contents of which are incorporated herein by reference.

Other types of nucleic acid sequence that may be deleted include prophages, pseudogenes, toxin genes, pathogenicity genes, periplasmic protein genes, membrane protein genes, and bacteriophage receptors, such as [tonA] (FhuA) and/or its complete operon [fhu] ABC which encodes the receptor for the lytic phage T1.

Other types of nucleic acid sequences that may be deleted may be identified by comparing the genome of one bacterial strain to one or more others strains. Nucleic acid sequences that are not present in two or three of the strains are less likely to be functionally essential and thus may be candidates for deletion. After the sequence of E. coli K-12 (see Blattner, et al., supra), was compared to the sequence of its close relative 0157:H7 (See Perna et al., supra), it was discovered that 22% (K-12) and 46% (O157:H7) of the protein encoding genes may be located on strain-specific islands of from one to about 85 kb inserted randomly into a relatively constant backbone. U.S. Patent Publication No. 20030138937 and International Patent Publication No. WO 2003/070880, the contents of which are incorporated herein by reference, describe the comparison of the genomic sequences of E. coli strains O157:H7 EDL933 and K-12 MG1655, which led to the identification and deletion of twelve targets resulting in a bacteria strain with a genome that is about 8% smaller. The bacteria with the reduced genome grew at substantially the same rate as the native parent MG1655 strain.

The DNA sequence of a uropathogenic E. coli strain CFT073 H7 (see Welch et al., supra), was recently determined and its sequence was compared to the K-12 (MG1655) and O157:H7. Results show that only about 40% of all coding genes found in any one of the genomes are present in all of the genomes and CFT073, K-12 and 157:H7 are composed of 67%, 43% and 68% strain specific island genes. Based on this information, as much as about 60% of the protein coding sequences may be deleted from E. coli. It should be noted that there may be genes essential for growth in one strain that are not required for growth in other strains. In such cases, the gene essential for growth of that strain may not be deleted from the strain or if deleted may be replaced with another gene with a complementary function so as to permit growth of the strain.

Other types of nucleic acid sequences that may be deleted are genes coding periplasmic proteins. Gram-negative bacteria, such as E. coli, have two cellular membranes, the inner cell membrane and the outer cell membrane, which are separated by a periplasmic space (PS). Nine known and three putative periplasmic protein genes were successfully deleted in constructing MDS40, without significantly affecting the ability of the organism to grow on minimal medium, as shown in International Patent Publication No. WO 2003/070880, the contents of which are incorporated herein by reference. These mutations affect a range of functions, including amino acid uptake, inorganic metabolism, cell membrane maintenance, sugar metabolism, and adhesion. Eliminating periplasmic proteins may reduce the contaminants in recombinant proteins expressed in the periplasm.

Approximately 85 genes have been deleted that code for known or putative membrane proteins, identified by their signal-peptide sequences. Of these 33 may be involved in flagellar structure or biosynthesis; 9 may be involved in fimbrial structure or biosynthesis; and 13 may be involved in general secretory pathways. The remainder may have a variety of known or putative functions in the cell membranes. Many of these proteins may be processed in the periplasmic space. They have also been deleted in constructing MDS40 without significantly affecting the ability of the organism to grow on minimal medium, as shown in International Patent Publication No. WO 2003/070880, the contents of which are incorporated herein by reference.

By searching for signal peptide-like sequences in annotated MG1655 databases, and cross-relating these with the literature, 181 proteins have been identified which may be resident periplasmic proteins. A number of these proteins have been classified according to function into several groups excluding: adhesion and mobility; nutrient and salt uptake, trace element uptake; environmental sensing; defense and protection; and periplasmic protein secretion and processing. Among the genes or full operons which may be deleted are those coding for sugar and amino acid transport proteins, which may not be needed in defined minimal media say for biopharmaceutical production.

One may test the consequence of deleting one or several genes or other nucleic acid sequences from the genome. For example, after one or several genes or other nucleic acid sequences of the genome have been deleted, one can measure the survival and proliferation rate of the resultant bacteria. Although most of the above-identified genes or other nucleic acid sequences may be deleted without detrimental effect for purpose of producing a desired product, it is possible that the deletion of a specific gene or other nucleic acid sequence may have an unacceptable consequence such as cell death or unacceptable level of reduction in proliferation rate. This possibility exists because of redundancies in gene functions and interactions between biological pathways. Some deletions that are viable in a strain without additional deletions will be deleterious only in combination with other deletions. The possibility exists also because of certain methods used to identify deletion candidates. For example, one method used to identify deletion candidates is to compare two E. coli strains and select genes or other DNA sequences that are not present in both strains. While the majority of these genes and other DNA sequences are not likely to be functionally essential, some of them may be important for a unique strain. Another method used to identify deletion candidates is to identify non-transcribed regions and the possibility exists that certain non-transcribed regions may be important for genome stability.

The consequence of deleting one or several genes or other DNA sequences to be tested depends on the purpose of an application. For example, when high production efficiency is the main concern, which is true for many applications, the effect of deletions on proliferation rate and medium consumption rate can be the consequence tested. In this case, the consequence tested can also be more specific as the production speed quantity and yield per cell of a particular product. When eliminating native protein contamination is the main concern, fewer native proteins and lower native protein levels, or the absence of a specific native protein, can be the consequence tested.

Testing the consequence of deleting a gene or other DNA sequence may be important when little is known about the gene or the DNA sequence. This is another viable method to identify deletion candidates in making a bacterium with a reduced genome. This method is particularly useful when candidates identified by other methods have been deleted and additional candidates are being sought.

When the consequence of deleting a gene or other DNA sequence has an effect on the viability of the bacteria under a set of conditions, one alternative to not deleting the specific gene or other DNA sequence is to determine if there are measures that can mitigate the detrimental effects. For example, if deleting lipopolysaccharide (LPS) genes results in poor survival due to more porous cellular membranes caused by the absence from the cellular membranes of the transmembrane domain of the LPS proteins, culture conditions can be changed to accommodate the more porous cellular membranes so that the bacteria lacking the LPS genes can survive just as well as the bacteria carrying the LPS genes.

c. Manner of Making Deletions

The strains may be made by deleting the nucleic acid sequences using any of the several methods known to those of skill in the art for deleting genomic nucleic acid. The nucleic acid sequences may be deleted from the genome without generating any other mutations at the deletion site and without leaving any inserted nucleic acid behind (scarless deletion). If several sequential deletions are being made from a bacterial genome, it may be important not to leave any inserted nucleic acid sequences behind. Such inserted sequences, if they were left behind, may be candidate sites for undesired recombination events that would delete uncharacterized and perhaps important portions of the remaining genome from the bacteria or cause other unanticipated genome rearrangements with untoward effects.

Representative methods for making deletions in the genome of a bacteria are described in U.S. Patent Publication No. 20030138937 and International Patent Publication No. WO 2003/070880, Posfai, G. et al., J. Bacteriol. 179: 4426-4428 (1997), Muyrers, J. P. P, et al., Nucl. Acids Res. 27:1555-1557 (1999), Datsenko, K. A. et al., Proc. Natl. Acad. Sci. 97:6640-6649 (2000) and Posfai, G. et al., Nucl. Acids Res. 27: 4409-4415 (1999), each of which is incorporated herein by reference. The deletion methods may be classified to those that are based on linear DNAs and those that are based on suicide plasmids. The methods disclosed in Muyrers, J. P. P. et al., Nucl. Acids Res. 27:1555-1557 (1999) and Datsenko, K. A. et al., Proc. Natl. Acad. Sci. 97:6640-6649 (2000) are linear DNA-based methods and the methods disclosed in Posfai, G. et al., J. Bacteriol. 179: 4426-4428 (1997) and Posfai, G. et al., Nucl. Acids Res. 27: 4409-4415 (1999) are suicide plasmid-based methods.

3. METHODS

a. Products

The reduced genome strain may used for the production of desired products, such as recombinant proteins, nucleic acids, therapeutic products, metabolic intermediates and end products. Representative examples of recombinant proteins include, but are not limited to, insulin, interleukins, cytokines, growth hormones, growth factors, erythropoietin, colony stimulating factors, interferon, antibodies and antibody fragments. Representative examples of therapeutic products include, but are not limited to, a vaccine component, a diagnostic product, or a research reagent. Representative examples of metabolic intermediates and end products include, but are not limited to, vanillin, shikimic acid, amino acids, vitamins, organic acids, and the like, and chemical compounds not naturally produced in the bacteria but produced as a result of metabolic pathway engineering or other genetic manipulation (see, e.g., U.S. Pat. Nos. 6,472,16 and 6,372,476, both of which are incorporated herein by reference).

Recombinant proteins may be expressed into the periplasm or cytoplasm. The expression of proteins in the periplasm is routinely used for industrial use and has been reviewed in Hanahan, [J. Mol. Biol.], 166:557-80, 1983; Hockney, [Trends Biotechnol.], 12:456-632, 1994; and Hannig et al., [Trends Biotechnol.], 16:54-60, 1998, each of which is incorporated herein by reference. Recombinant proteins may be produced in the periplasm by expressing fusion proteins in which they are attached to a signal peptide that causes secretion into the periplasmic space. There the signal peptide may be cleaved off by specific signal peptidases.

Constructs useful for periplasmic expression may comprise a first nucleic acid sequence encoding a signal peptide capable of mediating transport of a protein to the periplasmic space operatively linked to a second nucleic acid sequence encoding the desired protein. The signal sequence may be native to the protein being expressed. The protein transported into the periplasmic space may be biologically active. Expression of the recombinant construct may be under the control of an inducible promoter or a promoter that is constitutively expressed in the host strain. The use of inducible promoters may be advantageous when using the Sec system which may be saturable. For example, [lac]-based promoter/repressor, inducible by the non-metabolisable galactose derivative, IPTG, may be used. Such promoters may allow fine tuning of expression and secretion through the Sec system thereby optimizing periplasmic expression.

The recombinant protein may also be co-expressed with chaperones/disulfide-bond forming enzymes which may provide proper folding of the recombinant protein. Nucleic acid sequences useful for periplasmic expression of recombinant protein include, but are not limited to, those described in U.S. Pat. Nos. 5,747,662; 5,578,464; 6,335,178; and 6,022,952; Thomas et al., Mol-Micro[Mol Micro], (2001) 39 (1) 47-53; Weiner et al., [Cell], (1998) 93, 93-101; and Current Protocols in Molecular Biology[Current Protocols in Molecular Biology] (1994) 16.6.1-16.6.14 (Copyright 2000 by John Wiley [et al.] and Sons), each of which is incorporated herein by reference.

b. Amplification

The reduced genome strain may also be used to clone or amplify a nucleic acid. The reduced genome strain may provide a clean, minimal genetic background that may allow the production of high quality nucleic acids. A reduced genome strain lacking selfish DNA elements, such as IS elements, may also allow the cloning of otherwise toxic genes. The reduced genome strain may also be used to produce a library, such as a genomic library.

c. Vaccine

The reduced genome strain may also be used as a vaccine by introducing heterologous genes encoding antigens capable of inducing an immune response in a host that has been inoculated with the vaccine. Reduced genome vaccines may be DNA based vaccines containing a DNA known to be capable of inducing a desired physiological response in a host (i.e., immune response).

The present invention has multiple aspects, illustrated by the following non-limiting examples.

Example 1 Production of MDS40

Reduced genome strain MDS40 was produced as described in International Patent Publication No: WO 2003/070880, which is incorporated herein by reference. Briefly, a series of reduced genome strains (MDS01-MDS40) were produced by making a series of cumulative deletions of nucleic acid sequences from the parental strain E. coli MG1655 (annotated version m56) (SEQ ID NO: 1), including those regions shown in Table 2.

Example 2 Production of MDS41

Reduced genome strain MDS41 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the following nucleic acid region from parental strain MDS40, with reference to SEQ ID NO: 1:

Strain Type Coordinates Name B Function MDS41 CDS 167484 . . . 169727 fhuA b0150 outer membrane protein receptor for ferrichrome, colicin M, and phages T1, T5, and phi80 MDS41 CDS 169778 . . . 170575 fhuC b0151 ATP-binding component of hydroxymate- dependent iron transport MDS41 CDS 170575 . . . 171465 fhuD b0152 hydroxamate-dependent iron uptake cytoplasmic membrane component MDS41 CDS 171462 . . . 173444 fhuB b0153 hydroxamate-dependent iron uptake cytoplasmic membrane component

Example 3 Production of MDS42

Reduced genome strain MDS42 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the following nucleic acid region from parental strain MDS41, with reference to SEQ ID NO: 1:

Strain Type Coordinates Name B Function MDS42 CDS 3088369 . . . 3089076 endA b2945 DNA-specific endonuclease I

Example 4 Production of MDS42recA⁻

Reduced genome strain MDS42 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the following nucleic acid region from parental strain MDS42, with reference to SEQ ID NO: 1:

Strain Type Coordinates Name B Function MDS42recA CDS Complement recA b2699 DNA strand exchange and renaturation (2820730 . . . 2821791) DNA-dependent ATPase, DNA- and ATP- dependent coprotease

Example 5 Production of MDS43

Reduced genome strain MDS43 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the nucleic acid regions shown in Table 3 from parental strain MDS442, with reference to SEQ ID NO: 1.

Example 6 Production of MDS44

Reduced genome strain MDS44 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the following nucleic acid region (also show in Table 4) from parental strain MDS43, with reference to SEQ ID NO: 1:

Strain Type Coordinates Name B Function MDS44 CDS 2599223 . . . 2599840 hyfA b2481 hydrogenase 4 Fe-S subunit MDS44 CDS 2599840 . . . 2601858 hyfB b2482 hydrogenase 4 membrane subunit MDS44 CDS 2601869 . . . 2602816 hyfC b2483 hydrogenase 4 membrane subunit MDS44 CDS 2602833 . . . 2604272 hyfD b2484 hydrogenase 4 membrane subunit MDS44 CDS 2604284 . . . 2604934 hyfE b2485 hydrogenase 4 membrane subunit MDS44 CDS 2604939 . . . 2606519 hyfF b2486 hydrogenase 4 membrane subunit MDS44 CDS 2606509 . . . 2608176 hyfG b2487 hydrogenase 4 subunit MDS44 CDS 2608186 . . . 2608731 hyfH b2488 hydrogenase 4 Fe-S subunit MDS44 CDS 2608728 . . . 2609486 hyfI b2489 hydrogenase 4 Fe-S subunit MDS44 CDS 2609479 . . . 2609892 hyfJ b2490 hydrogenase 4 component J MDS44 CDS 2609922 . . . 2611934 hyfR b2491 putative 2-component regulator interaction with sigma 54 MDS44 CDS 2611956 . . . 2612804 focB b2492 probable formate transporter (formate channel 2)

Example 7 Production of MDS45

Reduced genome strain MDS45 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the following nucleic acid region (also show in Table 5) from parental strain MDS44, with reference to SEQ ID NO: 1:

Strain Type Coordinates Name B Function MDS45 CDS 2318065 . . . 2319891 atoS b2219 sensor protein AtoS for response regulator AtoC MDS45 CDS 2319888 . . . 2321273 atoC b2220 response regulator of ato, ornithine decarboxylase antizyme (sensor ATOS) MDS45 CDS 2321469 . . . 2322131 atoD b2221 acetyl-CoA: acetoacetyl-CoA transferase alpha subunit MDS45 CDS 2322131 . . . 2322781 atoA b2222 acetyl-CoA: acetoacetyl-CoA transferase beta subunit MDS45 CDS 2322778 . . . 2324100 atoE b2223 short chain fatty acid transporter MDS45 CDS 2324131 . . . 2325315 atoB b2224 acetyl-CoA acetyltransferase MDS45 CDS complement(2325389 . . . 2326165) yfaP b2225 orf, hypothetical protein MDS45 CDS complement(2326170 . . . 2327819) yfaQ b2226 orf, hypothetical protein MDS45 CDS complement(2327820 . . . 2328305) yfaS_2 b2227 orf, hypothetical protein MDS45 pseudogene complement(2327820 . . . 2332424) yfaS b4500 pseudogene MDS45 CDS complement(2328321 . . . 2332424) yfaS_1 b2228 putative membrane protein MDS45 CDS complement(2332358 . . . 2332981) yfaT b2229 orf, hypothetical protein MDS45 CDS complement(2332978 . . . 2334666) yfaA b2230 orf, hypothetical protein

Example 8 Production of MDS46

Reduced genome strain MDS46 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the following nucleic acid region (also show in Table 6) from parental strain MDS45, with reference to SEQ ID NO: 1:

Strain Type Coordinates Name B Function MDS46 CDS 3886458 . . . 3886532 tnaL b3707 tryptophanase leader peptide MDS46 CDS 3886753 . . . 3888168 tnaA b3708 tryptophanase MDS46 CDS 3888259 . . . 3889506 tnaB b3709 low affinity tryptophan permease MDS46 CDS 3889638 . . . 3890813 yidY b3710 putative transport protein MDS46 CDS 3890788 . . . 3891747 yidZ b3711 putative transcriptional regulator LYSR-type MDS46 CDS 3891892 . . . 3892653 yieE b3712 orf, hypothetical protein MDS46 CDS 3892675 . . . 3893241 yieF b3713 orf, hypothetical protein MDS46 CDS complement(3893295 . . . 3894632) yieG b3714 putative membrane/transport protein MDS46 CDS 3894797 . . . 3895462 yieH b3715 putative phosphatase MDS46 CDS 3895529 . . . 3895996 yieI b3716 orf, hypothetical protein MDS46 CDS 3896045 . . . 3896632 yieJ b3717 orf, hypothetical protein MDS46 CDS complement(3896694 . . . 3897416) yieK b3718 pyrimidine-specific nucleoside hydrolase MDS46 CDS complement(3897431 . . . 3898627) yieL b3719 putative xylanase MDS46 CDS complement(3898627 . . . 3900243) yieC b3720 putative receptor protein MDS46 CDS complement(3900312 . . . 3901724) bglB b3721 phospho-beta-glucosidase B; cryptic MDS46 CDS complement(3901743 . . . 3903620) bglF b3722 PTS system beta-glucosides, enzyme II, cryptic MDS46 CDS complement(3903754 . . . 3904590) bglG b3723 positive regulation of bgl operon

Example 9 Production of MDS47

Reduced genome strain MDS47 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the following nucleic acid region (also show in Table 7) from parental strain MDS46, with reference to SEQ ID NO: 1:

Strain Type Coordinates Name B Function MDS47 CDS complement(674793 . . . 675770) ybeQ b0644 orf, hypothetical protein MDS47 CDS 675934 . . . 676641 ybeR b0645 orf, hypothetical protein MDS47 CDS 676638 . . . 678065 ybeS b0646 putative enzyme of polynucleotide modification MDS47 CDS complement(678075 . . . 678629) ybeT b0647 orf, hypothetical protein MDS47 CDS 678731 . . . 679438 ybeU b0648 putative tRNA ligase MDS47 CDS 679435 . . . 680886 ybeV b0649 orf, hypothetical protein MDS47 CDS complement(680946 . . . 682616) hscC b0650 putative heatshock protein (Hsp70 family)

Example 10 Production of MDS48

Reduced genome strain MDS48 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the following nucleic acid region (also show in Table 8) from parental strain MDS47, with reference to SEQ ID NO: 1:

Strain Type Coordinates Name B Function MDS48 CDS complement(4295242 . . . 4297389) fdhF b4079 selenopolypeptide subunit of formate dehydrogenase H MDS48 CDS complement(4297587 . . . 4299053) yjcP b4080 putative enzyme MDS48 CDS complement(4299050 . . . 4301101) yjcQ b4081 putative enzyme MDS48 CDS complement(4301101 . . . 4302132) yjcR b4082 putative membrane protein MDS48 CDS complement(4302635 . . . 4304620) yjcS b4083 orf, hypothetical protein MDS48 CDS complement(4304893 . . . 4305822) alsK b4084 putative D-allose kinase MDS48 CDS complement(4305806 . . . 4306501) alsE b4085 allulose-6-phosphate 3-epimerase MDS48 CDS complement(4306512 . . . 4307492) alsE b4086 D-allose ABC transporter MDS48 CDS complement(4307471 . . . 4309003) alsA b4087 D-allose ABC transporter MDS48 CDS complement(4309130 . . . 4310065) alsB b4088 D-allose ABC transporter MDS48 CDS complement(4310124 . . . 4311014) rpiR b4089 transcriptional repressor of rpiB expression MDS48 CDS 4311373 . . . 4311822 rpiB b4090 ribose 5-phosphate isomerase B MDS48 CDS 4311891 . . . 4312220 yjdP b4487 conserved hypothetical protein MDS48 CDS complement(4312367 . . . 4313125) phnP b4092 phosphonate metabolism MDS48 CDS complement(4313127 . . . 4313561) phnO b4093 putative regulator, phn operon MDS48 CDS complement(4313548 . . . 4314105) phnN b4094 ATP-binding component of phosphonate transport MDS48 CDS complement(4314105 . . . 4315241) phnM b4095 phosphonate metabolism MDS48 CDS complement(4315238 . . . 4315918) phnL b4096 ATP-binding component of phosphonate transport MDS48 CDS complement(4316029 . . . 4316787) phnK b4097 ATP-binding component of phosphonate transport MDS48 CDS complement(4316784 . . . 4317629) phnJ b4098 phosphonate metabolism MDS48 CDS complement(4317622 . . . 4318686) phnI b4099 phosphonate metabolism MDS48 CDS complement(4318686 . . . 4319270) phnH b4100 phosphonate metabolism MDS48 CDS complement(4319267 . . . 4319719) phnG b4101 phosphonate metabolism MDS48 CDS complement(4319720 . . . 4320445) phnF b4102 putative transcriptional regulator MDS48 CDS complement(4320466 . . . 4320834) phnE_2 b4103 orf, hypothetical protein MDS48 CDS complement(4320684 . . . 4321304) phnE b4104 membrane channel protein component of Pn transporter MDS48 CDS complement(4321359 . . . 4322375) phnD b4105 periplasmic binding protein component of Pn transporter MDS48 CDS complement(4322400 . . . 4323188) phnC b4106 ATP-binding component of phosphonate transport

Example 11 Production of MDS49

Reduced genome strain MDS49 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the following nucleic acid region (also show in Table 9) from parental strain MDS48, with reference to SEQ ID NO: 1:

Strain Type Coordinates Name B Function MDS49 CDS complement(764376 . . . 765098) farR b0730 transcriptional regulator of succinyl-CoA synthetase operon MDS49 CDS 765207 . . . 767183 hrsA b0731 protein modification enzyme, induction of ompC MDS49 CDS 767201 . . . 769834 ybgG b0732 putative sugar hydrolase

Example 12 Production of MDS50

Reduced genome strain MDS450 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the following nucleic acid region (also show in Table 10) from parental strain MDS49, with reference to SEQ ID NO: 1:

Strain Type Coordinates Name B Function MDS50 CDS 3737728 . . . 3738981 avtA b3572 alanine-alpha- ketoisovalerate (or valine- pyruvate) transaminase, transaminase C MDS50 CDS complement(3739132 . . . 3739605) yiaI b3573 orf, hypothetical protein MDS50 CDS complement(3739707 . . . 3740555) yiaJ b3574 transcriptional repressor of the yiaK-S operon. MDS50 CDS 3740756 . . . 3741754 yiaK b3575 putative dehydrogenase MDS50 CDS 3741766 . . . 3742233 yiaL b3576 putative lipase MDS50 CDS 3742351 . . . 3742824 yiaM b3577 orf, hypothetical protein MDS50 CDS 3742827 . . . 3744104 yiaN b3578 putative membrane protein MDS50 CDS 3744117 . . . 3745103 yiaO b3579 putative solute-binding transport protein MDS50 CDS 3745107 . . . 3746603 lyxK b3580 L-xylulose kinase, cryptic MDS50 CDS 3746600 . . . 3747262 sgbH b3581 probable 3-hexulose 6- phosphate synthase MDS50 CDS 3747255 . . . 3748115 sgbU b3582 putative 3-hexulose-6- phosphate isomerase MDS50 CDS 3748109 . . . 3748804 sgbE b3583 L-ribulose-5-phosphate 4- epimerase MDS50 CDS complement(3749151 . . . 3749891) yiaT b3584 putative outer membrane protein MDS50 CDS 3750015 . . . 3750989 yiaU b3585 putative transcriptional regulator LYSR-type MDS50 CDS complement(3750986 . . . 3752122) yiaV b3586 putative membrane protein MDS50 CDS complement(3752128 . . . 3752451) yiaW b3587 orf, hypothetical protein

Example 13 Production of MDS51

Reduced genome strain MDS51 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the following nucleic acid region (also show in Table 11) from parental strain MDS50, with reference to SEQ ID NO: 1:

Strain Type Coordinates Name B Function MDS51 CDS 1727111 . . . 1731727 lhr b1653 member of ATP-dependent helicase superfamily II

Example 14 Production of MDS52

Reduced genome strain MDS52 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the following nucleic acid region (also show in Table 12) from parental strain MDS51, with reference to SEQ ID NO: 1:

Strain Type Coordinates Name B Function MD52 CDS complement (2858489 . . . 2859286) ygbI b2735 putative DEOR-type transcriptional regulator MD52 CDS 2859452 . . . 2860360 ygbJ b2736 putative dehydrogenase MD52 CDS 2860357 . . . 2861523 ygbK b2737 orf, hypothetical protein MD52 CDS 2861615 . . . 2862253 ygbL b2738 putative epimerase/aldolase MD52 CDS 2862258 . . . 2863034 ygbM b2739 orf, hypothetical protein MD52 CDS 2863123 . . . 2864487 ygbN b2740 putative transport protein

Example 15 Production of MDS53

Reduced genome strain MDS53 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the following nucleic acid region (also show in Table 13) from parental strain MDS52, with reference to SEQ ID NO: 1:

Strain Type Coordinates Name B Function MDS53 CDS complement(2519615 . . . 2520499) xapR b2405 regulator for xapA MDS53 CDS complement(2520751 . . . 2522007) xapB b2406 xanthosine permease MDS53 CDS complement(2522067 . . . 2522900) xapA b2407 xanthosine phosphorylase

Example 16 Production of MDS54

Reduced genome strain M4DS54 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the following nucleic acid region (also show in Table 14) from parental strain MDS53, with reference to SEQ ID NO: 1:

Strain Type Coordinates Name B Function MDS54 CDS 4085025 . . . 4086080 yiiG b3896 orf, hypothetical protein MDS54 CDS complement(4086130 . . . 4087878) frvR b3897 putative frv operon regulatory protein MDS54 CDS complement(4087878 . . . 4088948) frvX b3898 frv operon protein MDS54 CDS complement(4088938 . . . 4090389) frvB b3899 PTS system, fructose-like enzyme IIBC component MDS54 CDS complement(4090400 . . . 4090846) frvA b3900 PTS system, fructose-specific IIA component

Example 17 Production of MDS55

Reduced genome strain MDS55 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the following nucleic acid region (also show in Table 15) from parental strain MDS54, with reference to SEQ ID NO: 1:

Strain Type Coordinates Name B Function MDS55 CDS complement(1252308 . . . 1255175) yogV b1202 putative adhesion and penetration protein

Example 18 Production of MDS56

Reduced genome strain MDS56 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the following nucleic acid region (also show in Table 16) from parental strain MDS55, with reference to SEQ ID NO: 1:

Strain Type Coordinates Name B Function MDS56 CDS complement(4486584 . . . 4488086) yjgR b4263 orf, hypothetical protein MDS56 CDS complement(4488164 . . . 4489162) idnR b4264 L-idonate transcriptional regulator MDS56 CDS complement(4489229 . . . 4490548) idnT b4265 L-idonate transporter MDS56 CDS complement(4490610 . . . 4491374) idnO b4266 5-keto-D-gluconate 5- reductase MDS56 CDS complement(4491398 . . . 4492429) idnD b4267 L-idonate dehydrogenase MDS56 CDS 4492646 . . . 4493209 idnK b4268 gluconate kinase

Example 19 Production of MDS57

Reduced genome strain MDS57 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the following nucleic acid region (also show in Table 17) from parental strain MDS56, with reference to SEQ ID NO: 1:

Strain Type Coordinates Name B Function MDS57 CDS complement(643420 . . . 644226) rna b0611 RNase I, cleaves phosphodiester bond between any two nucleotides

Example 20 Production of MDS58

Reduced genome strain MDS58 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the following nucleic acid region (also show in Table 18) from parental strain MDS57, with reference to SEQ ID NO: 1:

Strain Type Coordinates Name B Function MDS58 CDS complement(2474716 . . . 2475651) dsdC b2364 D-serine dehydratase (deaminase) transcriptional activator MDS58 CDS 2475869 . . . 2477206 dsdX b2365 transport system permease (serine) MDS58 CDS 2477224 . . . 2478552 dsdA b2366 D-serine dehydratase (deaminase)

Example 21 Production of MDS59

Reduced genome strain MDS59 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the following nucleic acid region (also show in Table 19) from parental strain N4DS58, with reference to SEQ ID NO: 1:

Strain Type Coordinates Name B Function MDS59 CDS 879950 . . . 881152 dacC b0839 D-alanyl-D- alanine carboxypeptidase penicillin- binding protein 6

Example 22 Production of MDS60

Reduced genome strain MDS60 was produced using methods as described in International Patent Publication No. WO 2003/070880 by deleting the following nucleic acid region (also show in Table 20) from parental strain MDS59, with reference to SEQ ID NO: 1:

Strain Type Coordinates Name B Function MDS60 CDS complement(513625 . . . 514080) ybbJ b0488 orf, hypothetical protein MDS60 CDS complement(514080 . . . 514997) ybbK b0489 putative protease MDS60 CDS 515143 . . . 515820 ybbL b0490 putative ATP-binding component of a transport system MDS60 CDS 515807 . . . 516586 ybbM b0491 putative metal resistance protein MDS60 CDS complement(516649 . . . 517503) ybbN b0492 putative thioredoxin-like protein MDS60 CDS complement(517564 . . . 518373) ybbO b0493 putative oxidoreductase MDS60 CDS complement(518363 . . . 518989) tesA b0494 acyl-CoA thioesterase I; also functions as protease I MDS60 CDS 518957 . . . 519643 ybbA b0495 putative ATP-binding component of a transport system MDS60 CDS 519640 . . . 522054 ybbP b0496 putative oxidoreductase MDS60 CDS 522485 . . . 526765 rhsD b0497 rhsD protein in rhs element MDS60 CDS 526805 . . . 527173 ybbC b0498 orf, hypothetical protein MDS60 CDS 527173 . . . 527883 ylbH b0499 orf, hypothetical protein MDS60 CDS 527864 . . . 528124 ybbD b0500 orf, hypothetical protein MDS60 CDS complement(528869 . . . 529240) ylbJ b0502 orf, hypothetical protein

Example 23 Growth Rates of Reduced Genome Strains

Strains MDS41, MDS42, MDS43, MDS44, MDS45, MDS46, MDS47, MDS48, MDS49, MDS50, MDS51, MDS52, MDS53, MDS54, MDS55, MDS56, MDS57, MDS58, MDS59 and MDS60 were characterized for growth on standard microbiological media. Each of the MDS strains can be grown to high cell densities in fed-batch fermentations on minimal medium. In addition, the growth rates were essentially unchanged for each strain relative to the parental strain MG1655.

FIG. 2 shows a representative comparison of the growth rate of MG1655 and MDS42 in MOPS minimal medium at 37° C. Doubling time was obtained by measuring OD₆₀₀ of cultures grown in baffled flasks at 37° C. The log linear portion of the growth curve was used to calculate the average doubling times and standard deviation from six replicates on the plate. The doubling time of MG1655 was 61.3 minutes compared to a doubling time of 69.07 minutes for MDS42.

Example 24 Transformation Efficiency of Reduced Genome Strains

Strain MDS42 was compared to MG1655 for transformation efficiency. Cells were grown under standard growth conditions to optical density of 0.5 at 600 nm. Cell cultures were then centrifuged, cell pellets were washed several times with water and finally resuspended in water. Approximately 25 ng of either pUC19 or BAC DNA was added to approximately 100 μl of the cell suspension and subjected to electroporation using standard electroporation protocol, e.g., 1.8 kV and resistance of 150 ohms in a 0.1 cm electroporation cuvette using an Invitrogen Electroporator II™ device. As shown below, MDS42 has a substantially higher transformation efficiency compared to MG1655.

Electroporation Efficiency (transformants/μg DNA) Strain pUC19 (2.6 kb) BAC (150 kb) MDS42 4.0 × 10⁹ 8.6 × 10⁶ MG1655 3.0 × 10⁷ 3.3 × 10⁶

Example 25 Protein Expression in Reduced Genome Strains

FIG. 3 shows that expression of recombinant proteins was similar for the MDS and MG1655 strains based on expression of the model protein chloramphenicol acetyl transferase (CAT).

Example 26 DNA Production in Reduced Genome Strains

The results below show that the reduced genome strains are able to produce recombinant DNA with yields higher than the native parental strain and other commercial strains.

DNA Yields (ng/ml) Strain pUC19 PACYC184 MDS42 1305.6 487.8 MG1655 991.2 582.6 DH10B 1228.8 195.6

DNA Yields (mg/OD₆₀₀) Strain Plasmid A Plasmid B MDS42recA⁻ 4.97 6.26 DH10B 3.05 0.26

Example 27 DNA Stability in Reduced Genome Strains

FIG. 4 indicates that the measured frequency of mutation by IS hopping dropped to zero in the reduced genome strains. Interestingly, the frequency of IS insertion events increased by 2.5-fold when CAT was over expressed in MG1655. This indicates that the over-expression of recombinant proteins may induce IS transposition. The reduced genome strains may therefore be a more stable host for protein production.

Example 28 DNA Quality in Reduced Genome Strains

To test the quality of DNA obtained from the reduced genome strains, pBR322 isolated from MDS42 and commercially obtained pBR322 were tested for the presence of IS elements by PCR. FIG. 5 indicates that recombinant DNA of high quality may be obtained from the reduced genome strains.

Example 29 Cloning of Difficult to Clone Sequence in Reduced Genome Strains

MDS42 was used to prepare pCTXVP60. The plasmid DNA was then propagated in various hosts, isolated, then digested with NcoI and EcoRI. FIG. 6 shows a representative restriction pattern. The fragments were then purified and sequenced. MDS41 (lane 1) and MDS42 (lane 2) did not contain an insertion. DH10B contained an IS1 insertion (lane 3) and also an IS10 insertion/deletion (lane 4). C600 contained an IS5 insertion (lane 5), an IS1 insertion (lanes 6 and 7). This shows that the reduced genome strains may be used to clone sequences that would be difficult to clone (e.g., toxic genes) in other strains.

TABLE 1 Type S Left Right Name B CDS + 190 255 thrL b0001 CDS + 337 2799 thrA b0002 CDS + 2801 3733 thrB b0003 CDS + 3734 5020 thrC b0004 CDS + 5234 5530 yaaX b0005 CDS − 5683 6459 yaaA b0006 CDS − 6529 7959 yaaJ b0007 CDS + 8238 9191 talB b0008 CDS + 9306 9893 mog b0009 CDS − 9928 10494 yaaH b0010 CDS − 10643 11356 yaaW b0011 CDS + 10830 11315 htgA b0012 CDS − 11382 11786 yaaI b0013 CDS + 12163 14079 dnaK b0014 CDS + 14168 15298 dnaJ b0015 repeat_region + 15388 16730 CDS + 15445 16557 yi81_1 b0016 CDS − 15869 16177 yi82_1 b0017 CDS − 16751 16960 mokC b0018 CDS + 17489 18655 nhaA b0019 CDS + 18715 19620 nhaR b0020 repeat_region − 19796 20553 CDS − 19811 20314 insB_1 b0021 CDS − 20233 20508 insA_1 b0022 CDS − 20815 21078 rpsT b0023 CDS + 21181 21399 yaaY b0024 CDS + 21407 22348 ribF b0025 CDS + 22391 25207 ileS b0026 CDS + 25207 25701 lspA b0027 CDS + 25826 26275 fkpB b0028 CDS + 26277 27227 ispH b0029 CDS + 27293 28207 rihC b0030 CDS + 28374 29195 dapB b0031 CDS + 29651 30799 carA b0032 CDS + 30817 34038 carB b0033 CDS + 34300 34695 caiF b0034 CDS − 34781 35371 caiE b0035 CDS − 35377 36270 caiD b0036 CDS − 36271 37839 caiC b0037 CDS − 37898 39115 caiB b0038 CDS − 39244 40386 caiA b0039 CDS − 40417 41931 caiT b0040 CDS + 42403 43173 fixA b0041 CDS + 43188 44129 fixB b0042 CDS + 44180 45466 fixC b0043 CDS + 45463 45750 fixX b0044 CDS + 45807 47138 yaaU b0045 CDS + 47246 47776 yabF b0046 CDS + 47769 49631 kefC b0047 CDS + 49823 50302 folA b0048 CDS − 50380 51222 apaH b0049 CDS − 51229 51606 apaG b0050 CDS − 51609 52430 ksgA b0051 CDS − 52427 53416 pdxA b0052 CDS − 53416 54702 surA b0053 CDS − 54755 57109 imp b0054 CDS + 57364 58179 djlA b0055 CDS + 58474 59124 yabP b0056 CDS + 59121 59279 yabQ b0057 CDS − 59687 60346 rluA b0058 CDS − 60358 63264 hepA b0059 CDS − 63429 65780 polB b0060 CDS − 65855 66550 araD b0061 CDS − 66835 68337 araA b0062 CDS − 68348 70048 araB b0063 CDS + 70387 71265 araC b0064 CDS + 71351 72115 yabI b0065 CDS − 72229 72927 yabJ b0066 CDS − 72911 74521 yabK b0067 CDS − 74497 75480 tbpA b0068 CDS − 75644 77299 yabN b0069 CDS + 77621 78799 setA b0070 CDS − 78848 79453 leuD b0071 CDS − 79464 80864 leuC b0072 CDS − 80867 81958 leuB b0073 CDS − 81958 83529 leuA b0074 CDS − 83622 83708 leuL b0075 CDS + 84368 85312 leuO b0076 CDS + 85630 87354 ilvI b0077 CDS + 87357 87848 ilvH b0078 CDS + 87860 87946 fruL b0079 CDS + 88028 89032 fruR b0080 CDS + 89634 90092 yabB b0081 CDS + 90094 91035 yabC b0082 CDS + 91032 91397 ftsL b0083 CDS + 91413 93179 ftsI b0084 CDS + 93166 94653 murE b0085 CDS + 94650 96008 murF b0086 CDS + 96002 97084 mraY b0087 CDS + 97087 98403 murD b0088 CDS + 98403 99647 ftsW b0089 CDS + 99644 100711 murG b0090 CDS + 100765 102240 murC b0091 CDS + 102233 103153 ddlB b0092 CDS + 103155 103985 ftsQ b0093 CDS + 103982 105244 ftsA b0094 CDS + 105305 106456 ftsZ b0095 CDS + 106557 107474 lpxC b0096 CDS + 107705 108217 yacA b0097 CDS + 108279 110984 secA b0098 CDS + 111044 111433 mutT b0099 CDS − 111564 111698 b0100 CDS − 111649 111846 yacG b0101 CDS − 111856 112599 yacF b0102 CDS − 112599 113219 yacE b0103 CDS + 113444 114487 guaC b0104 CDS − 114407 114514 b0105 CDS − 114522 115724 hofC b0106 CDS − 115714 117099 hofB b0107 CDS − 117109 117549 ppdD b0108 CDS − 117752 118645 nadC b0109 CDS + 118733 119284 ampD b0110 CDS + 119281 120135 ampE b0111 CDS − 120178 121551 aroP b0112 CDS + 122092 122856 pdhR b0113 CDS + 123017 125680 aceE b0114 CDS + 125695 127587 aceF b0115 CDS + 127912 129336 lpdA b0116 CDS − 129407 131260 yacH b0117 CDS + 131615 134212 acnB b0118 CDS + 134388 134750 yacL b0119 CDS − 134788 135582 speD b0120 CDS − 135598 136464 speE b0121 CDS − 136570 136917 yacC b0122 CDS + 137083 138633 cueO b0123 CDS − 138835 141225 gcd b0124 CDS + 141431 141967 hpt b0125 CDS − 142008 142670 yadF b0126 CDS + 142779 143705 yadG b0127 CDS + 143702 144472 yadH b0128 CDS + 144577 145017 yadI b0129 CDS + 145081 146310 yadE b0130 CDS − 146314 146694 panD b0131 CDS + 146968 147870 yadD b0132 CDS − 147944 148795 panC b0133 CDS − 148807 149601 panB b0134 CDS − 149715 150953 yadC b0135 CDS − 151003 151599 yadK b0136 CDS − 151626 152231 yadL b0137 CDS − 152243 152812 yadM b0138 CDS − 152829 155426 htrE b0139 CDS − 155461 156201 ecpD b0140 CDS − 156299 156883 yadN b0141 CDS − 157253 157732 folK b0142 CDS − 157729 159126 pcnB b0143 CDS − 159186 160112 yadB b0144 CDS − 160149 160604 dksA b0145 CDS − 160782 161486 sfsA b0146 CDS − 161501 162031 ligT b0147 CDS + 162105 164534 hrpB b0148 CDS + 164730 167264 mrcB b0149 CDS + 167484 169727 fhuA b0150 CDS + 169778 170575 fhuC b0151 CDS + 170575 171465 fhuD b0152 CDS + 171462 173444 fhuB b0153 CDS − 173602 174882 hemL b0154 CDS + 175107 176528 yadQ b0155 CDS + 176610 176954 yadR b0156 CDS − 177001 177624 yadS b0157 CDS − 177662 178462 yadT b0158 CDS − 178455 179153 pfs b0159 CDS + 179237 180754 dgt b0160 CDS + 180884 182308 degP b0161 CDS + 182463 183620 cdaR b0162 CDS − 183709 184095 yaeH b0163 CDS − 184257 185069 yaeI b0164 CDS − 184987 185118 b0165 CDS − 185123 185947 dapD b0166 CDS − 185978 188650 glnD b0167 CDS − 188712 189506 map b0168 CDS + 189874 190599 rpsB b0169 CDS + 190857 191708 tsf b0170 CDS + 191855 192580 pyrH b0171 CDS + 192872 193429 frr b0172 CDS + 193521 194717 ispC b0173 CDS + 194903 195664 uppS b0174 CDS + 195677 196534 cdsA b0175 CDS + 196546 197898 yaeL b0176 CDS + 197928 200360 yaeT b0177 CDS + 200482 200967 hlpA b0178 CDS + 200971 201996 lpxD b0179 CDS + 202101 202556 fabZ b0180 CDS + 202560 203348 lpxA b0181 CDS + 203348 204496 lpxB b0182 CDS + 204493 205089 rnhB b0183 CDS + 205126 208608 dnaE b0184 CDS + 208621 209580 accA b0185 CDS + 209679 211820 ldcC b0186 CDS + 211877 212266 yaeR b0187 CDS + 212331 213629 tilS b0188 CDS − 213678 213932 rof b0189 CDS + 214291 214836 yaeQ b0190 CDS + 214833 215255 yaeJ b0191 CDS + 215269 215979 cutF b0192 CDS − 216179 217003 yaeF b0193 CDS − 217057 218775 proS b0194 CDS − 218887 219594 yaeB b0195 CDS − 219591 219995 rcsF b0196 CDS − 220113 220928 metQ b0197 CDS − 220968 221621 metI b0198 CDS − 221614 222645 metN b0199 CDS + 222833 223408 gmhB b0200 rRNA + 223771 225312 rrsH b0201 tRNA + 225381 225457 ileV b0202 tRNA + 225500 225575 alaV b0203 rRNA + 225759 228662 rrlH b0204 rRNA + 228756 228875 rrfH b0205 tRNA + 228928 229004 aspU b0206 CDS + 229167 229970 dkgB b0207 CDS − 229967 230881 yafC b0208 CDS + 231122 231922 yafD b0209 CDS + 231926 232549 yafE b0210 CDS − 232597 233955 mltD b0211 CDS − 234027 234782 gloB b0212 CDS + 234816 235538 yafS b0213 CDS − 235535 236002 rnhA b0214 CDS + 236067 236798 dnaQ b0215 tRNA + 236931 237007 aspV b0216 CDS + 237335 238120 yafT b0217 CDS − 238746 239084 yafU b0218 CDS − 239419 240189 yafV b0219 CDS + 240343 240816 ivy b0220 CDS − 240859 243303 fadE b0221 CDS + 243543 244121 lpcA b0222 CDS + 244327 245094 yafJ b0223 CDS − 245065 245805 yafK b0224 CDS − 245961 246239 yafQ b0225 CDS − 246242 246502 dinJ b0226 CDS + 246712 247461 yafL b0227 CDS + 247637 248134 yafM b0228 CDS − 248358 250070 fhiA b0229 CDS + 250072 250827 mbhA b0230 CDS + 250898 251953 dinB b0231 CDS + 252005 252298 yafN b0232 CDS + 252301 252699 yafO b0233 CDS + 252709 253161 yafP b0234 CDS + 253467 253733 ykfJ b0235 CDS + 253702 254202 prfH b0236 CDS − 254259 255716 pepD b0237 CDS + 255977 256435 gpt b0238 CDS + 256527 257771 yafA b0239 CDS + 257829 256230 crl b0240 CDS − 258269 259324 phoE b0241 CDS + 259612 260715 proB b0242 CDS + 260727 261980 proA b0243 tRNA + 262095 262170 thrW b0244 CDS − 262552 262893 ykfI b0245 CDS − 262914 263231 yafW b0246 CDS − 263480 263956 ykfG b0247 CDS − 263972 264430 yafX b0248 CDS − 264528 264767 ykfF b0249 CDS − 264844 265311 ykfB b0250 CDS − 265334 266191 yafY b0251 CDS − 266408 267229 yafZ b0252 CDS − 267321 268184 ykfA b0253 CDS − 268513 269406 perR b0254 repeat_region + 269430 269994 CDS + 269466 269870 yi91a b0255 repeat_region + 269765 270985 CDS + 269827 270978 tra8_1 b0256 repeat_region + 270930 271751 CDS + 271054 271479 b0257 CDS + 272071 273216 ykfC b0258 repeat_region + 273179 274373 CDS − 273325 274341 trs5_1 b0259 CDS + 274549 275952 mmuP b0260 CDS + 275939 276871 mmuM b0261 CDS − 276980 278026 afuC b0262 CDS − 278038 278400 afuB b0263 repeat_region − 278387 279154 CDS − 278402 278905 insB_2 b0264 CDS − 278824 279099 insA_2 b0265 repeat_region + 279155 279335 CDS − 279609 279959 yagB b0266 CDS − 280053 281207 yagA b0267 CDS + 281481 282410 yagE b0268 CDS + 282425 284392 yagF b0269 CDS + 284619 286001 yagG b0270 CDS + 286013 287623 yagH b0271 CDS − 287628 288386 yagI b0272 CDS − 288525 289529 argF b0273 repeat_region − 289858 290625 CDS − 289873 290376 insB_3 b0274 CDS − 290295 290570 insA_3 b0275 CDS + 290724 291455 yagJ b0276 CDS − 291546 292172 yagK b0277 CDS − 292444 293142 yagL b0278 CDS − 293169 294023 yagM b0279 CDS − 294363 294803 yagN b0280 CDS − 294920 296320 intF b0281 CDS − 296605 297015 yagP b0282 CDS − 296994 297950 yagQ b0283 CDS − 297960 300158 yagR b0284 CDS − 300155 301111 yagS b0285 CDS − 301108 301797 yagT b0286 CDS + 302215 302829 yagU b0287 CDS − 303077 303406 ykgJ b0288 CDS − 303719 304429 aygV b0289 CDS − 304398 306041 yagW b0290 CDS − 306031 308556 yagX b0291 CDS − 308582 309250 yagY b0292 CDS − 309308 309895 yagZ b0293 CDS − 309970 310560 ykgK b0294 CDS + 311336 311563 ykgL b0295 CDS − 311738 312001 ykgM b0296 CDS + 313581 314468 eaeH b0297 repeat_region + 314453 315707 CDS + 314506 314814 b0298 CDS + 314811 315677 tra5_5 b0299 CDS − 315674 316360 ykgA b0300 CDS − 316950 317543 ykgB b0301 CDS − 317555 317791 ykgI b0303 CDS − 317900 319225 ykgC b0304 CDS + 319451 320305 ykgD b0305 CDS + 320832 321551 ykgE b0306 CDS + 321562 322989 ykgF b0307 CDS + 322982 323677 ykgG b0308 CDS − 323632 323844 b0309 CDS − 323920 324588 ykgH b0310 CDS − 324801 326471 betA b0311 CDS − 326485 327957 betB b0312 CDS − 327971 328558 betI b0313 CDS + 328687 330720 betT b0314 CDS + 331595 332683 yahA b0315 CDS − 332725 333657 yahB b0316 CDS − 333749 334246 yahC b0317 CDS + 334504 335109 yahD b0318 CDS + 335149 336012 yahE b0319 CDS + 336002 337549 yahF b0320 CDS + 337549 338967 yahG b0321 CDS + 339389 340339 yahI b0323 CDS + 340349 341731 yahJ b0324 CDS + 342108 343157 yahK b0325 CDS + 343400 344215 yahL b0326 CDS + 344628 344873 YahM b0327 CDS − 344890 345561 yahN b0328 CDS + 345708 345983 yahO b0329 CDS − 346081 347667 prpR b0330 CDS + 347906 348796 prpB b0331 CDS + 349236 350405 prpC b0333 CDS + 350439 351890 prpD b0334 CDS + 351930 353816 prpE b0335 CDS + 354146 355405 codB b0336 CDS + 355395 356678 codA b0337 CDS − 357015 357914 cynR b0338 CDS + 358023 358682 cynT b0339 CDS + 358713 359183 cynS b0340 CDS + 359216 360370 cynX b0341 CDS − 360473 361084 lacA b0342 CDS − 361150 362403 lacY b0343 CDS − 362455 365529 lacZ b0344 CDS − 365652 366734 lacI b0345 CDS − 366811 367758 mhpR b0346 CDS + 367835 369499 mhpA b0347 CDS + 369501 370445 mhpB b0348 CDS + 370448 371329 mhpC b0349 CDS + 371339 372148 mhpD b0350 CDS + 372145 373095 mhpF b0351 CDS + 373092 374105 mhpE b0352 CDS + 374683 375894 mhpT b0353 CDS + 375996 376535 yaiL b0354 CDS − 376759 377592 frmB b0355 CDS − 377686 378795 frmA b0356 CDS − 378830 379105 frmR b0357 CDS − 379293 380066 yaiO b0358 CDS − 380068 380283 b0359 repeat_region − 380484 381814 CDS + 380530 380940 yi21_1 b0360 CDS + 380898 381803 yi22_1 b0361 CDS − 381728 382114 b0362 CDS − 381963 383159 yaiP b0363 CDS − 383283 383840 yaiS b0364 CDS + 384456 385418 tauA b0365 CDS + 385431 386198 tauB b0366 CDS + 386195 387022 tauC b0367 CDS + 387019 387870 tauD b0368 CDS − 387977 388951 hemB b0369 CDS − 389121 389339 b0370 CDS + 389475 390935 yaiT b0371 repeat_region − 390933 392190 CDS − 390963 391829 tra5_1 b0372 CDS − 391826 392134 b0373 CDS + 392239 393642 yaiU b0374 CDS + 393685 394353 yaiV b0375 CDS − 394354 395511 ampH b0376 CDS + 395863 397083 sbmA b0377 CDS + 397096 398190 yaiW b0378 CDS − 398249 398557 yaiY b0379 CDS + 398817 399029 yaiZ b0380 CDS − 399053 400147 ddlA b0381 CDS + 400610 400870 yaiB b0382 CDS + 400971 402386 phoA b0383 CDS + 402505 402825 psiF b0384 CDS + 402927 404042 yaiC b0385 CDS − 404059 404868 proC b0386 CDS + 404988 405446 yaiI b0387 CDS + 405629 406153 aroL b0388 CDS + 406203 406394 yaiA b0389 CDS + 406652 407329 aroM b0390 CDS + 407401 407685 yaiE b0391 CDS + 407893 408174 ykiA b0392 CDS − 408332 409243 yaiD b0393 CDS + 409368 410276 yajF b0394 CDS − 410521 411705 araJ b0396 CDS − 411831 414977 sbcC b0397 CDS − 414974 416176 sbcD b0398 CDS + 416366 417055 phoB b0399 CDS + 417113 418408 phoR b0400 CDS + 418815 420134 brnQ b0401 CDS + 420210 421583 proY b0402 CDS + 421739 423556 malZ b0403 CDS − 423561 424142 yajB b0404 CDS + 424235 425305 queA b0405 CDS + 425361 426488 tgt b0406 CDS + 426511 426843 yajC b0407 CDS + 426871 428718 secD b0408 CDS + 428729 429700 secF b0409 CDS + 429829 430176 yajD b0410 CDS − 430353 431237 tsx b0411 CDS − 431536 432075 yajI b0412 CDS + 432226 432675 ybaD b0413 CDS + 432679 433782 ribD b0414 CDS + 433871 434341 ribE b0415 CDS + 434361 434780 nusB b0416 CDS + 434858 435835 thiL b0417 CDS + 435813 436331 pgpA b0418 CDS − 436385 437359 yajO b0419 CDS − 437539 439401 dxs b0420 CDS − 439426 440325 ispA b0421 CDS − 440325 440567 xseB b0422 CDS + 440773 442221 thiI b0423 CDS − 442275 442865 thiJ b0424 CDS − 442828 443739 apbA b0425 CDS + 443907 444398 yajQ b0426 CDS − 444526 445890 yajR b0427 CDS − 446039 446929 cyoE b0428 CDS − 446941 447270 cyoD b0429 CDS − 447270 447884 cyoC b0430 CDS − 447874 449865 cyoB b0431 CDS − 449887 450834 cyoA b0432 CDS − 451294 452769 ampG b0433 CDS − 452813 453391 yajG b0434 CDS + 453696 454013 bolA b0435 CDS + 454357 455655 tig b0436 CDS + 455901 456524 clpP b0437 CDS + 456650 457924 clpX b0438 CDS + 458112 460466 lon b0439 CDS + 460675 460947 hupB b0440 CDS + 461139 463010 ppiD b0441 CDS + 463161 463532 ybaV b0442 CDS + 463626 464024 ybaW b0443 CDS − 464076 464771 ybaX b0444 CDS − 464836 466536 ybaE b0445 CDS + 466636 467454 cof b0446 CDS + 467607 468065 ybaO b0447 CDS + 468095 469867 mdlA b0448 CDS + 469860 471641 mdlB b0449 CDS + 471822 472160 glnK b0450 CDS + 472190 473476 amtB b0451 CDS − 473525 474385 tesB b0452 CDS + 474603 475175 ybaY b0453 CDS − 475206 475595 ybaZ b0454 misc_RNA + 475672 475785 ffs b0455 CDS + 475896 476249 ybaA b0456 CDS − 476291 477841 ylaB b0457 CDS − 478005 478475 ylaC b0458 CDS − 478591 479142 maa b0459 CDS − 479314 479532 hha b0460 CDS − 479558 479932 ybaJ b0461 CDS − 480478 483627 acrB b0462 CDS − 483650 484843 acrA b0463 CDS + 484985 485632 acrR b0464 CDS + 485760 489122 kefA b0465 CDS − 489334 489495 ybaM b0466 CDS − 489509 490036 priC b0467 CDS + 490106 490483 ybaN b0468 CDS + 490636 491187 apt b0469 CDS + 491316 493247 dnaX b0470 CDS + 493300 493629 ybaB b0471 CDS + 493629 494234 recR b0472 CDS + 494344 496218 htpG b0473 CDS + 496399 497043 adk b0474 CDS + 497279 498241 hemH b0475 CDS − 498238 499197 aes b0476 CDS + 499349 500653 gsk b0477 CDS − 500786 502462 ybaL b0478 CDS − 502700 503920 fsr b0479 CDS + 504138 505790 ushA b0480 CDS − 505827 506306 ybaK b0481 CDS − 506510 507304 ybaP b0482 CDS + 507442 507783 ybaQ b0483 CDS − 508099 510603 copA b0484 CDS + 510865 511797 ybaS b0485 CDS + 511800 513092 ybaT b0486 CDS + 513217 513624 cueR b0487 CDS − 513625 514080 ybbJ b0488 CDS − 514080 514997 ybbK b0489 CDS + 515143 515820 ybbL b0490 CDS + 515807 516586 ybbM b0491 CDS − 516649 517503 ybbN b0492 CDS − 517564 518373 ybbO b0493 CDS − 518363 518989 tesA b0494 CDS + 518957 519643 ybbA b0495 CDS + 519640 522054 ybbP b0496 CDS + 522485 526765 rhsD b0497 CDS + 526805 527173 ybbC b0498 CDS + 527173 527883 ylbH b0499 CDS + 527864 528124 ybbD b0500 CDS + 528163 528354 b0501 CDS − 528869 529240 ylbG b0502 CDS − 529356 530450 ybbB b0503 CDS − 530519 531445 ybbS b0504 CDS + 531675 532157 allA b0505 CDS + 532235 533050 allR b0506 CDS + 533140 534921 gcl b0507 CDS + 534934 535710 hyi b0508 CDS + 535810 536688 glxR b0509 CDS + 536720 536998 ybbV b0510 CDS + 536857 538311 allP b0511 CDS + 538371 539732 allB b0512 CDS + 539789 541090 ybbY b0513 CDS + 541112 542257 glxK b0514 CDS − 542485 543270 ylbA b0515 CDS − 543281 544516 allC b0516 CDS − 544538 545587 allD b0517 CDS + 545904 547571 fdrA b0518 CDS + 547838 548839 ylbE b0519 CDS + 548850 549665 ylbF b0520 CDS + 549662 550555 arcC b0521 CDS − 550750 551817 purK b0522 CDS − 551814 552323 purE b0523 CDS − 552441 553163 ybbF b0524 CDS − 553166 553660 ppiB b0525 CDS + 553834 555219 cysS b0526 CDS − 555255 555776 ybcI b0527 CDS − 555884 556096 ybcJ b0528 CDS − 556098 556964 folD b0529 CDS + 557435 557977 sfmA b0530 CDS + 558197 558889 sfmC b0531 CDS + 558920 561523 sfmD b0532 CDS + 561565 562542 sfmH b0533 CDS + 562553 563068 sfmF b0534 CDS − 563071 563703 fimZ b0535 tRNA + 563946 564022 argU b0536 CDS − 564038 565201 intD b0537 CDS + 565195 565755 b0538 CDS − 565321 565584 ybcC b0539 repeat_region + 566000 567257 CDS + 566056 566364 b0540 CDS + 566361 567227 tra5_2 b0541 CDS + 567285 567470 b0542 CDS + 567538 567870 emrE b0543 CDS + 568125 569651 ybcK b0544 CDS + 570116 570667 ybcL b0545 CDS + 570677 571474 ybcM b0546 CDS + 571689 572144 ybcN b0547 CDS + 572144 572314 ninE b0548 CDS + 572307 572597 ybcO b0549 CDS + 572594 572956 rus b0550 CDS + 573179 573562 ybcQ b0551 repeat_region + 573814 575008 CDS − 573960 574976 trs5_2 b0552 CDS − 574981 576108 nmpC b0553 CDS + 576621 576836 ybcR b0554 CDS + 576836 577333 ybcS b0555 CDS + 577330 577791 ybcT b0556 CDS − 577823 578116 ybcU b0557 CDS − 578407 578817 ybcV b0558 CDS + 579103 579309 ybcW b0559 CDS + 580057 580602 nohB b0560 CDS + 580757 581320 ybcX b0561 CDS − 581375 581959 ybcY b0562 CDS + 582098 582283 ylcE b0563 CDS + 582904 583653 appY b0564 CDS − 583903 584856 ompT b0565 CDS − 585370 586131 envY b0566 CDS − 586314 587204 ybcH b0567 CDS − 587205 590177 nfrA b0568 CDS − 590164 592401 nfrB b0569 CDS − 592551 593993 cusS b0570 CDS − 593983 594666 cusR b0571 CDS + 594823 596196 cusC b0572 CDS + 596354 596686 ylcC b0573 CDS + 596702 597925 cusB b0574 CDS + 597937 601080 cusA b0575 CDS + 601182 602556 pheP b0576 CDS − 602639 603886 ybdG b0577 CDS − 603994 604647 nfnB b0578 CDS − 604741 605109 ybdF b0579 CDS − 605174 605422 ybdJ b0580 CDS − 605488 606606 ybdK b0581 repeat_region + 607231 608573 CDS + 607288 608400 yi81_2 b0582 CDS − 608682 609311 entD b0583 CDS − 609477 611717 fepA b0584 CDS + 612038 613162 fes b0585 CDS + 613380 617261 entF b0586 CDS + 617477 618610 fepE b0587 CDS − 618607 619422 fepC b0588 CDS − 619419 620411 fepG b0589 CDS − 620408 621412 fepD b0590 CDS + 621523 622773 ybdA b0591 CDS − 622777 623733 fepB b0592 CDS + 624108 625283 entC b0593 CDS + 625293 626903 entE b0594 CDS + 626917 627774 entB b0595 CDS + 627774 628520 entA b0596 CDS + 628523 628936 ybdB b0597 CDS + 629117 631222 cstA b0598 CDS − 631612 632700 ybdH b0599 CDS + 632809 633969 ybdL b0600 CDS − 633970 634599 ybdM b0601 CDS − 634572 635792 ybdN b0602 CDS − 635939 636841 ybdO b0603 CDS − 637050 637796 dsbG b0604 CDS + 638168 638731 ahpC b0605 CDS + 638976 640541 ahpF b0606 CDS − 640662 641090 ybdQ b0607 CDS + 641311 642549 ybdR b0608 CDS + 642630 643091 b0609 CDS − 642780 643190 rnk b0610 CDS − 643420 644226 rna b0611 CDS − 644340 645803 citT b0612 CDS − 645854 646732 citG b0613 CDS − 646707 647258 ybdU b0614 CDS − 647262 648794 citF b0615 CDS − 648805 649713 citE b0616 CDS − 649710 650006 citD b0617 CDS − 650021 651079 citC b0618 CDS + 651458 653116 citA b0619 CDS + 653085 653765 citB b0620 CDS − 653806 665191 dcuC b0621 CDS + 655780 656340 crcA b0622 CDS + 656515 656724 cspE b0623 CDS − 656778 657161 crcB b0624 CDS + 657254 657481 ybeH b0625 CDS + 657478 658041 ybeM b0626 CDS + 658170 658373 tatE b0627 CDS − 658474 659439 lipA b0628 CDS − 659648 660601 ybeE b0629 CDS − 660860 661501 IipB b0630 CDS − 661602 661865 ybeD b0631 CDS − 661975 663186 dacA b0632 CDS − 663325 664413 rlpA b0633 CDS − 664424 665536 mrdB b0634 CDS − 665539 667440 mrdA b0635 CDS − 667471 667938 ybeA b0636 CDS − 667942 668259 ybeB b0637 CDS − 668519 669130 phpB b0638 CDS − 669154 669795 nadD b0639 CDS − 669797 670828 holA b0640 CDS − 670828 671409 rlpB b0641 CDS − 671424 674006 leuS b0642 CDS + 674241 674723 ybeL b0643 CDS − 674793 675770 ybeQ b0644 CDS + 675934 676641 ybeR b0645 CDS + 676638 678065 ybeS b0646 CDS − 678075 678629 ybeT b0647 CDS + 678731 679438 ybeU b0648 CDS + 679435 680886 ybeV b0649 CDS − 680946 682616 hscC b0650 CDS − 682700 683635 rihA b0651 CDS − 683753 684478 gltL b0652 CDS − 684478 685152 gltK b0653 CDS − 685152 685892 gltJ b0654 CDS − 686062 686970 gltI b0655 repeat_region + 687074 688268 CDS − 687220 688236 trs5_3 b0656 CDS − 688566 690104 lnt b0657 CDS − 690129 691007 ybeX b0658 CDS − 691097 691564 ybeY b0659 CDS − 691561 692640 ybeZ b0660 CDS − 692754 694178 yleA b0661 CDS + 694324 695499 ubiF b0662 tRNA − 695653 695727 glnX b0664 tRNA − 695765 695839 glnV b0665 tRNA − 695887 695963 metU b0666 tRNA − 695979 696053 glnW b0668 tRNA − 696088 696162 glnU b0670 tRNA − 696186 696270 leuW b0672 tRNA − 696280 696356 metT b0673 CDS − 696736 698400 asnB b0674 CDS − 698797 699549 nagD b0675 CDS − 699597 700817 nagC b0676 CDS − 700826 701974 nagA b0677 CDS − 702034 702834 nagB b0678 CDS + 703167 705113 nagE b0679 CDS + 705316 706980 glnS b0680 CDS + 707557 708963 ybfM b0681 CDS + 709013 709339 ybfN b0682 CDS − 709423 709869 fur b0683 CDS − 710158 710688 fldA b0684 CDS − 710828 711121 ybfE b0685 CDS − 711261 712025 ybfF b0686 CDS + 712210 712755 seqA b0687 CDS + 712781 714421 pgm b0688 CDS + 714635 715129 ybfP b0689 CDS − 715170 715580 ybfG b0690 CDS − 715611 715820 ybfH b0691 CDS − 716169 717488 potE b0692 CDS − 717485 719683 speF b0693 CDS − 720279 720956 kdpE b0694 CDS − 720953 723637 kdpD b0695 CDS − 723630 724202 kdpC b0696 CDS − 724211 726259 kdpB b0697 CDS − 726282 727955 KdpA b0698 CDS + 728357 728563 ybfA b0699 CDS + 728806 732999 rhsC b0700 CDS + 732593 732814 b0701 CDS + 732999 733325 ybfB b0702 CDS + 733443 734876 ybfO b0703 CDS + 734873 735442 ybfC b0704 CDS + 736123 737184 ybfL b0705 CDS + 737315 738076 ybfD b0706 CDS + 738224 738733 ybgA b0707 CDS + 738730 740148 phrB b0708 CDS − 740298 741779 ybgH b0709 CDS + 742050 742793 ybgI b0710 CDS + 742816 743472 ybgJ b0711 CDS + 743466 744398 ybgK b0712 CDS + 744388 745122 ybgL b0713 CDS + 745158 745949 nei b0714 CDS − 745946 746992 abrB b0715 CDS − 747144 748205 ybgO b0716 CDS − 748202 748930 ybgP b0717 CDS − 748945 751392 ybgQ b0718 CDS − 751452 752018 ybgD b0719 CDS − 752408 753691 gltA b0720 CDS + 754400 754789 sdhC b0721 CDS + 754783 755130 sdhD b0722 CDS + 755130 756896 sdhA b0723 CDS + 756912 757628 sdhB b0724 CDS + 757687 757947 b0725 CDS + 757929 760730 sucA b0726 CDS + 760745 761962 sucB b0727 CDS + 762237 763403 sucC b0728 CDS + 763403 764272 sucD b0729 CDS − 764376 765098 farR b0730 CDS + 765207 767183 hrsA b0731 CDS + 767201 769834 ybgG b0732 CDS + 770681 772249 cydA b0733 CDS + 772265 773404 cydB b0734 CDS + 773532 773825 ybgE b0735 CDS + 773975 774379 ybgC b0736 CDS + 774376 775068 tolQ b0737 CDS + 775072 775500 tolR b0738 CDS + 775565 776830 tolA b0739 CDS + 776963 778255 tolB b0740 CDS + 778290 778811 pal b0741 CDS + 778821 779612 ybgF b0742 tRNA + 779777 779852 lysT b0743 tRNA + 779988 780063 valT b0744 tRNA + 780066 780141 lysW b0745 tRNA + 780291 780366 valZ b0746 tRNA + 780370 780445 lysY b0747 tRNA + 780592 780667 lysZ b0748 tRNA + 780800 780875 lysQ b0749 CDS + 781308 782351 nadA b0750 CDS + 782389 783108 pnuC b0751 CDS − 783105 784046 ybgR b0752 CDS − 784160 784540 ybgS b0753 CDS + 784856 785908 aroG b0754 CDS − 786066 786818 gpmA b0755 CDS − 787020 788060 galM b0756 CDS − 788054 789202 galK b0757 CDS − 789206 790252 galT b0758 CDS − 790262 791278 galE b0759 CDS − 791539 793011 modF b0760 CDS − 793079 793867 modE b0761 CDS + 793996 794145 ybhT b0762 CDS + 794312 795085 modA b0763 CDS + 795085 795774 modB b0764 CDS + 795777 796835 modC b0765 CDS − 796836 797654 ybhA b0766 CDS + 797809 798804 ybhE b0767 CDS − 798845 799861 ybhD b0768 CDS + 799982 801034 ybhH b0769 CDS + 801110 802543 ybhI b0770 CDS + 802726 804987 ybhJ b0771 CDS − 805221 806504 ybhC b0772 CDS − 806656 807132 ybhB b0773 CDS − 807191 808480 bioA b0774 CDS + 808567 809607 bioB b0775 CDS + 809604 810758 bioF b0776 CDS + 810745 811500 bioC b0777 CDS + 811493 812170 bioD b0778 CDS + 812749 814770 uvrB b0779 CDS − 814962 815870 ybhK b0780 CDS + 816267 817256 moaA b0781 CDS + 817278 817790 moaB b0782 CDS + 817793 818278 moaC b0783 CDS + 818271 818516 moaD b0784 CDS + 818518 818970 moaE b0785 CDS + 819107 819811 ybhL b0786 CDS + 820016 820729 ybhM b0787 CDS − 820765 821721 ybhN b0788 CDS − 821721 822962 ybhO b0789 CDS − 822959 823720 ybhP b0790 CDS + 823853 824263 ybhQ b0791 CDS − 824225 825331 ybhR b0792 CDS − 825342 826475 ybhS b0793 CDS − 826468 828204 ybhF b0794 CDS − 828197 829195 ybhG b0795 CDS − 829195 829866 ybiH b0796 CDS + 830095 831459 rhlE b0797 CDS − 831691 832173 ybiA b0798 CDS + 832293 834443 dinG b0799 CDS + 834471 835433 ybiB b0800 CDS + 835574 836659 ybiC b0801 CDS − 836888 837148 ybiJ b0802 CDS − 837413 837679 ybiI b0803 CDS − 837753 838430 ybiX b0804 CDS − 838472 840754 ybiL b0805 CDS − 841019 841279 ybiM b0806 CDS + 841555 842481 ybiN b0807 CDS − 842478 844703 ybiO b0808 CDS − 844964 845686 glnQ b0809 CDS − 845683 846342 glnP b0810 CDS − 846481 847227 glnH b0811 CDS − 847631 848134 dps b0812 CDS − 848433 849320 ybiF b0813 CDS + 849673 850188 ompX b0814 CDS − 850237 851820 ybiP b0815 CDS + 851894 852163 b0816 CDS + 852406 852873 mntR b0817 CDS + 852870 853988 ybiR b0818 CDS − 854047 854967 ybiS b0819 CDS + 855186 856778 ybiT b0820 CDS − 857019 858284 ybiU b0821 CDS − 858436 859251 ybiV b0822 CDS − 859397 861829 ybiW b0823 CDS − 861835 862761 ybiY b0824 CDS + 862865 863527 fsa b0825 CDS − 863603 864352 moeB b0826 CDS − 864352 865587 moeA b0827 CDS + 865791 866756 ybiK b0828 CDS + 866776 868614 yliA b0829 CDS + 868634 870172 yliB b0830 CDS + 870190 871110 yliC b0831 CDS + 871113 872024 yliD b0832 CDS + 872202 874550 yliE b0833 CDS + 874558 875886 yliF b0834 CDS − 875933 877258 yliG b0835 CDS + 877471 877854 yliH b0836 CDS + 877965 879080 yliI b0837 CDS − 879077 879703 yliJ b0838 CDS + 879950 881152 dacC b0839 CDS − 881199 881957 deoR b0840 CDS − 882015 882611 ybjG b0841 CDS + 882896 884128 cmr b0842 CDS − 884169 884453 ybjH b0843 CDS − 884539 885354 ybjI b0844 CDS − 885354 886562 ybjJ b0845 CDS + 886646 887182 ybjK b0846 CDS − 887357 889042 ybjL b0847 CDS + 889312 889689 ybjM b0848 CDS − 889719 889976 grxA b0849 CDS + 890136 890423 ybjC b0850 CDS + 890407 891129 nfsA b0851 CDS + 891190 892092 rimK b0852 CDS + 892180 892656 ybjN b0853 CDS + 893007 894119 potF b0854 CDS + 894214 895347 potG b0855 CDS + 895357 896310 potH b0856 CDS + 896307 897152 potI b0857 CDS + 897212 897700 ybjO b0858 CDS + 897741 898868 ybjF b0859 CDS − 899067 899798 artJ b0860 CDS − 900089 900757 artM b0861 CDS − 900757 901473 artQ b0862 CDS − 901480 902211 artI b0863 CDS − 902229 902957 artP b0864 CDS − 903175 903690 ybjP b0865 CDS + 903816 904139 ybjQ b0866 CDS + 904136 904966 ybjR b0867 CDS − 904963 905976 ybjS b0868 CDS − 906075 907505 ybjT b0869 CDS − 907516 908517 ltaE b0870 CDS − 908554 910272 poxB b0871 CDS − 910405 911373 hcr b0872 CDS − 911385 913037 hcp b0873 CDS − 913181 914080 ybjE b0874 CDS − 914575 915270 aqpZ b0875 CDS + 915696 917354 ybjD b0876 CDS − 917351 918343 ybjX b0877 CDS + 918458 919573 macA b0878 CDS + 919570 921516 macB b0879 CDS − 921589 921813 cspD b0880 CDS + 922136 922456 yljA b0881 CDS + 922487 924763 clpA b0882 tRNA − 925107 925194 serW b0883 CDS − 925448 925666 infA b0884 CDS − 925951 926655 aat b0885 CDS − 926697 928418 cydC b0886 CDS − 928419 930185 cydD b0887 CDS − 930308 931273 trxB b0888 CDS + 931818 932312 lrp b0889 CDS + 932447 936436 ftsK b0890 CDS + 936592 937206 lolA b0891 CDS + 937217 938560 ycaJ b0892 CDS + 938651 939943 serS b0893 CDS + 940182 942626 dmsA b0894 CDS + 942637 943254 dmsB b0895 CDS + 943256 944119 dmsC b0896 CDS − 944154 944780 ycaC b0897 CDS + 945094 946242 ycaD b0898 CDS + 946452 947882 ycaM b0899 CDS − 947883 948791 ycaN b0900 CDS + 948891 949481 ycaK b0901 CDS − 949563 950303 pflA b0902 CDS − 950495 952777 pflB b0903 CDS − 952832 953689 focA b0904 CDS − 954095 955855 ycaO b0905 CDS + 955985 956677 ycaP b0906 CDS + 956876 957964 serC b0907 CDS + 958035 959318 aroA b0908 CDS + 959487 960251 ycaL b0909 CDS + 960424 961107 cmk b0910 CDS + 961218 962891 rpsA b0911 CDS + 963051 963335 himD b0912 CDS + 963543 965807 ycal b0913 CDS + 965844 967592 msbA b0914 CDS + 967589 968575 lpxK b0915 CDS + 968612 969844 ycaQ b0916 CDS + 969896 970078 ycaR b0917 CDS + 970075 970821 kdsB b0918 CDS + 970975 971868 ycbJ b0919 CDS − 971845 972624 ycbC b0920 CDS + 972760 973545 smtA b0921 CDS + 973542 974864 mukF b0922 CDS + 974872 975549 mukE b0923 CDS + 975549 980009 mukB b0924 CDS + 980270 982117 ycbB b0925 CDS + 982298 982846 ycbK b0926 CDS + 982873 983520 ycbL b0927 CDS − 983742 984932 aspC b0928 CDS − 985117 986205 ompF b0929 CDS − 986808 988208 asnS b0930 CDS − 988377 989579 pncB b0931 CDS + 989845 992457 pepN b0932 CDS − 992500 993267 ssuB b0933 CDS − 993264 994055 ssuC b0934 CDS − 994066 995211 ssuD b0935 CDS − 995208 996167 ssuA b0936 CDS − 996160 996735 ssuE b0937 CDS + 997091 997630 ycbQ b0938 CDS + 997713 998414 ycbR b0939 CDS − 998439 1001039 ycbS b0940 CDS + 1001030 1002100 ycbT b0941 CDS + 1002112 1002654 ycbU b0942 CDS + 1002662 1003177 ycbV b0943 CDS + 1003170 1003880 ycbF b0944 CDS + 1003991 1005001 pyrD b0945 CDS + 1005175 1005717 ycbW b0946 CDS − 1005714 1006823 ycbX b0947 CDS + 1007067 1009175 ycbY b0948 CDS + 1009187 1011094 uup b0949 CDS + 1011224 1012477 pqiA b0950 CDS + 1012482 1014122 pqiB b0951 CDS + 1014134 1014682 ymbA b0952 CDS + 1014938 1015105 rmf b0953 CDS − 1015175 1015693 fabA b0954 CDS − 1015762 1017522 ycbZ b0955 CDS + 1017708 1018160 ycbG b0956 CDS − 1018236 1019276 ompA b0957 CDS − 1019633 1020142 sulA b0958 CDS + 1020361 1020990 yccR b0959 CDS − 1020953 1023106 yccS b0960 CDS − 1023125 1023571 yccF b0961 CDS + 1023694 1025748 helD b0962 CDS − 1025780 1026238 mgsA b0963 CDS − 1026334 1026996 yccT b0964 CDS + 1027169 1027582 yccU b0965 CDS − 1027627 1027995 yccV b0966 CDS − 1028002 1029105 yccW b0967 CDS + 1029287 1029565 yccX b0968 CDS − 1029562 1029891 yccK b0969 CDS − 1029982 1030641 yccA b0970 tRNA − 1030848 1030935 serT b0971 CDS + 1031362 1032480 hyaA b0972 CDS + 1032477 1034270 hyaB b0973 CDS + 1034289 1034996 hyaC b0974 CDS + 1034993 1035580 hyaD b0975 CDS + 1035577 1035975 hyaE b0976 CDS + 1035972 1036829 hyaF b0977 CDS + 1036963 1038507 appC b0978 CDS + 1038519 1039655 appB b0979 CDS + 1039840 1041138 appA b0980 CDS − 1041253 1043433 yccC b0981 CDS − 1043453 1043899 yccY b0982 CDS − 1043887 1045026 yccZ b0983 CDS − 1045072 1047168 ymcA b0984 CDS − 1047168 1047914 ymcB b0985 CDS − 1047911 1048555 ymcC b0986 CDS − 1048662 1048967 ymcD b0987 repeat_region + 1049001 1049768 CDS + 1049250 1049753 insB_4 b0988 CDS − 1050186 1050398 cspH b0989 CDS + 1050684 1050896 cspG b0990 CDS + 1051070 1051300 sfa b0991 CDS − 1051512 1052585 yccM b0992 CDS − 1052657 1055401 torT b0993 CDS + 1055484 1056512 torT b0994 CDS − 1056485 1057177 torR b0995 CDS + 1057307 1058479 torC b0996 CDS + 1058479 1061025 torA b0997 CDS + 1061022 1061621 torD b0998 CDS − 1061773 1062078 yccD b0999 CDS − 1062078 1062998 cbpA b1000 CDS + 1063259 1064515 yccE b1001 CDS + 1064808 1066049 agp b1002 CDS − 1066087 1066314 yccJ b1003 CDS − 1066335 1066931 wrbA b1004 CDS + 1067141 1067371 ycdF b1005 CDS − 1067734 1069062 ycdG b1006 CDS − 1069083 1069577 ycdH b1007 CDS − 1069588 1070178 ycdI b1008 CDS − 1070188 1070988 ycdJ b1009 CDS − 1070996 1071382 ycdK b1010 CDS − 1071394 1072086 ycdL b1011 CDS − 1072086 1073234 ycdM b1012 CDS + 1073465 1074103 ycdC b1013 CDS − 1074143 1078105 putA b1014 CDS + 1078528 1080036 putP b1015 CDS + 1080579 1080689 ycdN_1 b1016 CDS + 1080677 1081408 ycdN_2 b1017 CDS + 1081466 1082593 ycdO b1018 CDS + 1082599 1083870 ycdB b1019 CDS + 1084215 1085279 phoH b1020 CDS − 1085329 1085742 ycdP b1021 CDS − 1085744 1087069 ycdQ b1022 CDS − 1087062 1089080 ycdR b1023 CDS − 1089089 1091512 ycdS b1024 CDS + 1092099 1093457 ycdT b1025 repeat_region − 1093468 1094725 CDS − 1093498 1094364 tra5_3 b1026 CDS − 1094361 1094669 b1027 CDS + 1094767 1095069 b1028 CDS + 1095066 1096052 ycdU b1029 tRNA − 1096788 1096875 serX b1032 CDS + 1097109 1098047 ycdW b1033 CDS + 1098102 1098839 ycdX b1034 CDS + 1098863 1099417 ycdY b1035 CDS + 1099519 1100010 ycdZ b1036 CDS − 1100074 1100907 csgG b1037 CDS − 1100934 1101350 csgF b1038 CDS − 1101375 1101764 csgE b1039 CDS − 1101769 1102419 csgD b1040 CDS + 1103174 1103629 csgB b1041 CDS + 1103670 1104125 csgA b1042 CDS + 1104184 1104516 csgC b1043 CDS + 1104637 1104948 ymdA b1044 CDS + 1105043 1105576 ymdB b1045 CDS + 1105578 1106999 ymdC b1046 CDS − 1107007 1108164 mdoC b1047 CDS + 1108558 1110093 mdoG b1048 CDS + 1110086 1112629 mdoH b1049 CDS + 1112802 1113029 yceK b1050 CDS − 1113030 1113404 msyB b1051 CDS − 1113434 1113532 b1052 CDS − 1113487 1114713 yceE b1053 CDS − 1114885 1115805 htrB b1054 CDS + 1116030 1117082 yceA b1055 CDS − 1117124 1117699 yceI b1056 CDS − 1117703 1118269 yceJ b1057 CDS − 1118530 1118670 yceO b1058 CDS − 1118691 1119809 solA b1059 CDS − 1119924 1120178 yceP b1060 CDS − 1120465 1120710 dinI b1061 CDS − 1120784 1121830 pyrC b1062 CDS − 1121936 1122496 yceB b1063 CDS − 1122630 1123277 grxB b1064 CDS − 1123341 1124549 yceL b1065 CDS + 1124785 1125369 rimJ b1066 CDS + 1125380 1126027 yceH b1067 CDS + 1126029 1126952 mviM b1068 CDS + 1127062 1128597 mviN b1069 CDS − 1128637 1129053 flgN b1070 CDS − 1129058 1129351 flgM b1071 CDS − 1129427 1130086 flgA b1072 CDS + 1130241 1130657 flgB b1073 CDS + 1130661 1131065 flgC b1074 CDS + 1131077 1131772 flgD b1075 CDS + 1131797 1133005 flgE b1076 CDS + 1133025 1133780 flgF b1077 CDS + 1133952 1134734 flgG b1078 CDS + 1134787 1135485 flgH b1079 CDS + 1135497 1136594 flgI b1080 CDS + 1136594 1137535 flgJ b1081 CDS + 1137601 1139244 flgK b1082 CDS + 1139256 1140209 flgL b1083 CDS − 1140405 1143590 rne b1084 CDS + 1143725 1144045 b1085 CDS + 1144163 1145122 rluC b1086 CDS − 1145234 1145818 yceF b1087 CDS + 1146017 1146538 yceD b1088 CDS + 1146590 1146763 rpmF b1089 CDS + 1146844 1147914 plsX b1090 CDS + 1147982 1148935 fabH b1091 CDS + 1148951 1149880 fabD b1092 CDS + 1149893 1150627 fabG b1093 CDS + 1150838 1151074 acpP b1094 CDS + 1151162 1152403 fabF b1095 CDS + 1152523 1153332 pabC b1096 CDS + 1153335 1154357 yceG b1097 CDS + 1154347 1154988 tmk b1098 CDS + 1154985 1155989 holB b1099 CDS + 1156000 1156797 ycfH b1100 CDS + 1157092 1158525 ptsG b1101 CDS − 1158585 1160774 fhuE b1102 CDS + 1161108 1161467 ycfF b1103 CDS + 1161470 1161847 ycfL b1104 CDS + 1161861 1162502 ycfM b1105 CDS + 1162483 1163307 ycfN b1106 CDS + 1163318 1164343 nagZ b1107 CDS + 1164366 1164908 ycfP b1108 CDS + 1165308 1166612 ndh b1109 CDS + 1166822 1167361 ycfJ b1110 CDS − 1167423 1168055 ycfQ b1011 CDS + 1168296 1168553 ycfR b1112 CDS − 1168635 1169597 ycfS b1113 CDS − 1169741 1173187 mfd b1114 CDS − 1173315 1174388 ycfT b1115 CDS + 1174650 1175849 ycfU b1116 CDS + 1175842 1176543 ycfV b1117 CDS + 1176543 1177787 ycfW b1118 CDS + 1177816 1178727 ycfX b1119 CDS + 1178743 1179582 cobB b1120 CDS − 1179702 1180490 ycfZ b1121 CDS − 1180487 1180948 ymfA b1122 CDS − 1181006 1182052 potD b1123 CDS − 1182049 1182843 potC b1124 CDS − 1182840 1183667 potB b1125 CDS − 1183681 1184817 potA b1126 CDS + 1185067 1186293 pepT b1127 CDS − 1186342 1187463 ycfD b1128 CDS − 1187539 1188999 phoQ b1129 CDS − 1188999 1189670 phoP b1130 CDS − 1189839 1191209 purB b1131 CDS − 1191213 1191854 ycfC b1132 CDS − 1191890 1192996 trmU b1133 CDS − 1193050 1193511 ymfB b1134 CDS − 1193521 1194174 ymfC b1135 CDS + 1194346 1195596 icdA b1136 CDS − 1196090 1196755 ymfD b1137 CDS − 1196756 1197460 ymfE b1138 CDS + 1197918 1198811 lit b1139 CDS − 1198902 1200029 intE b1140 CDS − 1200010 1200255 b1141 CDS − 1200292 1200603 ymfH b1142 CDS + 1200720 1201061 ymfI b1143 CDS − 1200999 1201307 ymfJ b1144 CDS − 1201482 1202156 ymfK b1145 CDS + 1202247 1202447 b1146 CDS + 1202479 1203048 ymfL b1147 CDS + 1203045 1203383 ymfM b1148 CDS + 1203393 1204760 ymfN b1149 CDS + 1204772 1204954 ymfR b1150 CDS + 1204954 1205427 ymfO b1151 CDS + 1205483 1206145 ymfP b1152 CDS + 1206136 1206720 ymfQ b1153 CDS + 1206724 1207353 ycfK b1154 CDS + 1207355 1207768 ymfS b1155 CDS − 1207740 1208342 tfaE b1156 CDS − 1208342 1208878 stfE b1157 CDS + 1208908 1209462 pin b1158 CDS + 1209569 1210402 mcrA b1159 CDS − 1210903 1211226 ycgW b1160 CDS − 1211926 1212330 ycgX b1161 CDS − 1212551 1213282 ycgE b1162 CDS − 1213487 1214698 ycgF b1163 CDS + 1215012 1215248 ycgZ b1164 CDS + 1215291 1215563 ymgA b1165 CDS + 1215592 1215858 ymgB b1166 CDS + 1215971 1216219 ymgC b1167 CDS + 1216551 1218074 ycgG b1168 CDS + 1220024 1220344 ycgH_1 b1169 CDS + 1220429 1221445 ycgH_2 b1170 CDS − 1221528 1221857 ymgD b1171 CDS − 1221867 1222142 b1172 CDS + 1222918 1223130 b1173 CDS − 1223502 1223768 minE b1174 CDS − 1223772 1224584 minD b1175 CDS − 1224608 1225303 minC b1176 CDS + 1225823 1226191 ycgJ b1177 CDS − 1226294 1226695 ycgK b1178 CDS + 1226904 1227230 ycgL b1179 CDS + 1227302 1227961 ycgM b1180 CDS + 1228053 1228499 ycgN b1181 CDS − 1228706 1229617 hlyE b1182 CDS + 1229990 1230409 umuD b1183 CDS + 1230409 1231677 umuC b1184 CDS − 1231723 1232253 dsbB b1185 CDS − 1232399 1233940 nhaB b1186 CDS + 1234161 1234880 fadR b1187 CDS − 1234932 1236464 ycgB b1188 CDS + 1236794 1238092 dadA b1189 CDS + 1238102 1239172 dadX b1190 CDS − 1239558 1241294 ycgO b1191 CDS − 1241389 1242303 ldcA b1192 CDS + 1242403 1243014 mltE b1193 CDS − 1243016 1243750 ycgR b1194 CDS + 1243951 1244205 ymgE b1195 CDS + 1244383 1244823 ycgY b1196 CDS − 1244902 1246599 treA b1197 CDS − 1246919 1248337 dhaM b1198 CDS − 1248348 1248980 dhaL b1199 CDS − 1248991 1250061 dhaK b1200 CDS + 1250289 1252208 dhaR b1201 CDS − 1252308 1255175 ycgV b1202 CDS − 1255944 1257035 ychF b1203 CDS − 1257152 1257736 pth b1204 CDS + 1258014 1258292 ychH b1205 CDS − 1258347 1260026 ychM b1206 CDS − 1260151 1261098 prsA b1207 CDS − 1261249 1262100 ispE b1208 CDS − 1262100 1262723 hemM b1209 CDS + 1262937 1264193 hemA b1210 CDS + 1264235 1265317 prfA b1211 CDS + 1265317 1266150 hemK b1212 CIJS + 1266147 1266539 ychQ b1213 CDS + 1266543 1267352 ychA b1214 CDS + 1267388 1268242 kdsA b1215 CDS − 1269972 1271072 chaA b1216 CDS + 1271342 1271572 chaB b1217 CDS + 1271709 1272425 chaC b1218 CDS − 1272469 1272822 ychN b1219 CDS + 1273148 1274401 ychP b1220 CDS − 1274402 1275052 narL b1221 CDS − 1275045 1276841 narX b1222 CDS + 1277180 1278571 narK b1223 CDS + 1279087 1282830 narG b1224 CDS + 1282827 1284365 narH b1225 CDS + 1284362 1285072 narJ b1226 CDS + 1285072 1285749 narI b1227 CDS + 1285932 1286207 b1228 CDS − 1286310 1286411 tpr b1229 tRNA − 1286467 1286551 tyrV b1230 tRNA − 1286761 1286845 tyrT b1231 CDS − 1287005 1287847 purU b1232 CDS − 1287897 1288355 ychJ b1233 CDS + 1288429 1289373 ychK b1234 CDS + 1289465 1290478 hnr b1235 CDS + 1290680 1291588 galU b1236 CDS − 1291732 1292145 hns b1237 CDS + 1292750 1293367 tdk b1238 CDS − 1293649 1294239 ychG b1239 CDS − 1294191 1294421 b1240 CDS − 1294669 1297344 adhE b1241 CDS + 1297821 1298468 ychE b1242 CDS + 1299206 1300837 oppA b1243 CDS + 1300923 1301843 oppB b1244 CDS + 1301858 1302766 oppC b1245 CDS + 1302778 1303791 oppD b1246 CDS + 1303788 1304792 oppF b1247 CDS − 1304845 1305174 yciU b1248 CDS − 1305209 1306669 cls b1249 CDS − 1307040 1308293 kch b1250 CDS − 1308593 1308889 yciI b1251 CDS + 1309113 1309832 tonB b1252 CDS − 1309872 1310270 yciA b1253 CDS − 1310375 1310914 ispZ b1254 CDS − 1310944 1311687 yciC b1255 CDS + 1312044 1312682 yciD b1256 CDS − 1312742 1313248 yciE b1257 CDS − 1313294 1313794 yciF b1258 CDS − 1313880 1314059 yciG b1259 CDS − 1314440 1315246 trpA b1260 CDS − 1315246 1316439 trpB b1261 CDS − 1316451 1317809 trpC b1262 CDS − 1317813 1319408 trpD b1263 CDS − 1319408 1320970 trpE b1264 CDS − 1321062 1321106 trpL b1265 CDS + 1321244 1322125 yciV b1266 CDS + 1322122 1322742 yciO b1267 CDS + 1322770 1324665 yciQ b1268 CDS + 1324876 1325751 yciL b1269 CDS − 1325791 1326381 btuR b1270 CDS − 1326378 1327136 yciK b1271 CDS + 1327356 1328405 sohB b1272 CDS − 1328441 1328692 yciN b1273 CDS + 1329072 1331669 topA b1274 CDS + 1331879 1332853 cysB b1275 CDS + 1333855 1336530 acnA b1276 CDS − 1336594 1337184 ribA b1277 CDS + 1337354 1338118 pgpB b1278 CDS + 1338267 1338575 yciS b1279 CDS + 1338582 1339751 yciM b1280 CDS + 1339945 1340682 pyrF b1281 CDS + 1340682 1341008 yciH b1282 CDS − 1341134 1341352 osmB b1283 CDS − 1341621 1342370 yciT b1284 CDS − 1342781 1344766 yciR b1285 CDS − 1345002 1346936 rnb b1286 CDS − 1347004 1348131 yciW b1287 CDS − 1348275 1349063 fabI b1288 CDS − 1349431 1349784 ycjD b1289 CDS − 1349852 1350658 sapF b1290 CDS − 1350660 1351652 sapD b1291 CDS − 1351652 1352542 sapC b1292 CDS − 1352529 1353494 sapB b1293 CDS − 1353491 1355134 sapA b1294 CDS − 1355447 1355692 ymjA b1295 CDS − 1355826 1357211 ycjJ b1296 CDS − 1357514 1358932 ycjK b1297 CDS + 1359144 1359908 ycjL b1298 CDS + 1359935 1360492 ycjC b1299 CDS + 1360767 1362254 aldH b1300 CDS + 1362256 1363536 ordL b1301 CDS + 1363574 1364839 goaG b1302 CDS − 1364959 1365936 pspF b1303 CDS + 1366103 1366771 pspA b1304 CDS + 1366825 1367049 pspB b1305 CDS + 1367049 1367408 pspC b1306 CDS + 1367417 1367638 pspD b1307 CDS + 1367713 1368027 pspE b1308 CDS + 1368240 1369919 ycjM b1309 CDS + 1369933 1371225 ycjN b1310 CDS + 1371246 1372127 ycjO b1311 CDS + 1372114 1372956 ycjP b1312 CDS + 1372987 1374039 ycjQ b1313 CDS + 1374058 1374846 ycjR b1314 CDS + 1374856 1375911 ycjS b1315 CDS + 1375908 1378175 ycjT b1316 CDS + 1378172 1378831 ycjU b1317 CDS + 1378845 1379813 ycjV b1318 CDS + 1379971 1380876 ompG b1319 CDS − 1380987 1381985 ycjW b1320 CDS + 1382141 1383538 ycjX b1321 CDS + 1383535 1384596 ycjF b1322 CDS + 1384744 1386285 tyrR b1323 CDS − 1386329 1386835 tpx b1324 CDS + 1386954 1387919 ycjG b1325 CDS − 1387894 1388682 ycjI b1326 CDS − 1388957 1389889 ycjY b1327 CDS + 1390015 1390914 ycjZ b1328 CDS + 1391251 1392864 mppA b1329 CDS − 1392915 1393946 ynaI b1330 repeat_region − 1394068 1395262 CDS + 1394100 1395116 trs5_4 b1331 CDS + 1395389 1395646 ynaJ b1332 CDS − 1395696 1396646 ydaA b1333 CDS − 1396798 1397550 fnr b1334 CDS − 1397745 1398260 ogt b1335 CDS − 1398271 1399797 abgT b1336 CDS − 1399834 1401279 abgB b1337 CDS − 1401279 1402589 abgA b1338 CDS + 1402765 1403673 abgR b1339 CDS + 1404003 1404566 ydaL b1340 CDS − 1404587 1405819 ydaM b1341 CDS + 1406074 1407057 ydaN b1342 CDS + 1407535 1408908 dbpA b1343 CDS − 1409037 1409972 ydaO b1344 CDS − 1410024 1411259 intR b1345 CDS − 1411261 1411476 xisR b1346 CDS − 1411555 1411764 ydaC b1347 CDS − 1411757 1411951 lar b1348 CDS − 1412008 1412817 recT b1349 CDS − 1412810 1415410 recE b1350 CDS − 1415512 1415787 racC b1351 CDS − 1416032 1416253 kil b1352 CDS + 1416695 1417183 sieB b1353 CDS + 1417192 1417368 b1354 CDS − 1417346 1417480 ydaG b1355 CDS − 1417789 1418265 racR b1356 CDS + 1418389 1418685 ydaS b1357 CDS + 1418708 1419130 ydaT b1358 CDS + 1419143 1420000 ydaU b1359 CDS + 1420007 1420753 ydaV b1360 CDS + 1420776 1421336 ydaW b1361 CDS + 1421369 1421668 rzpR b1362 CDS + 1421806 1423263 trkG b1363 CDS + 1423202 1423483 b1364 CDS + 1423401 1423664 ynaK b1365 CDS + 1423645 1424004 ydaY b1366 CDS + 1424079 1424312 b1367 CDS + 1424478 1425506 ynaA b1368 CDS + 1425482 1425637 b1369 repeat_region + 1425623 1426818 CDS − 1425770 1426750 trs5_5 b1370 CDS + 1426838 1427008 b1371 CDS + 1427073 1430435 stfR b1372 CDS + 1430435 1431010 tfaR b1373 CDS − 1431108 1431698 pinR b1374 CDS − 1432015 1432281 ynaE b1375 CDS − 1433209 1433643 ynaF b1376 CDS − 1433784 1434917 ompN b1377 CDS − 1435284 1438808 ydbK b1378 CDS − 1439345 1439767 hslJ b1379 CDS − 1439878 1440867 ldhA b1380 CDS + 1441075 1443714 ydbH b1381 CDS + 1443711 1443896 ynbE b1382 CDS + 1443904 1444230 ydbL b1383 CDS − 1444402 1445307 feaR b1384 CDS + 1445543 1447042 feaB b1385 CDS − 1447100 1449373 tynA b1386 CDS − 1449621 1451666 paaZ b1387 CDS + 1451951 1452880 paaA b1388 CDS + 1452892 1453179 paaB b1389 CDS + 1453188 1453934 paaC b1390 CDS + 1453949 1454446 paaD b1391 CDS + 1454454 1455524 paaE b1392 CDS + 1455521 1456288 paaF b1393 CDS + 1456288 1457076 paaG b1394 CDS + 1457078 1458505 paaH b1395 CDS + 1458495 1458917 paaI b1396 CDS + 1458917 1460122 paaJ b1397 CDS + 1460149 1461462 paaK b1398 CDS + 1461563 1462513 paaX b1399 CDS + 1462495 1463085 paaY b1400 CDS + 1463416 1465974 ydbA_1 b1401 repeat_region + 1465934 1467264 CDS − 1465945 1466850 yi22_2 b1402 CDS − 1466808 1467218 yi21_2 b1403 repeat_region + 1467320 1468540 CDS + 1467382 1468533 tra8_2 b1404 CDS + 1468714 1472037 ydbA_2 b1405 CDS + 1472245 1473105 ydbC b1406 CDS + 1473168 1475474 ydbD b1407 CDS + 1475645 1476250 ynbA b1408 CDS + 1476250 1477146 ynbB b1409 CDS + 1477162 1478919 ynbC b1410 CDS + 1478933 1480225 ynbD b1411 CDS − 1480279 1480884 acpD b1412 CDS + 1481142 1484987 hrpA b1413 CDS + 1485259 1486059 ydcF b1414 CDS + 1486256 1487695 aldA b1415 CDS − 1487737 1487988 gapC_2 b1416 CDS − 1487985 1488737 gapC_1 b1417 CDS + 1488926 1489456 cybB b1418 CDS + 1489701 1489874 ydcA b1419 CDS − 1489986 1490153 mokB b1420 CDS + 1490494 1492134 trg b1421 CDS − 1492172 1493095 ydcI b1422 CDS + 1493312 1494655 ydcJ b1423 CDS + 1494910 1496535 ydcG b1424 CDS − 1496456 1496659 b1425 CDS + 1496675 1496899 ydcH b1426 CDS + 1496962 1497501 rimL b1427 CDS − 1497493 1498473 ydcK b1428 CDS + 1498597 1499589 tehA b1429 CDS + 1499586 1500179 tehB b1430 CDS + 1500481 1501149 ydcL b1431 CDS + 1501681 1502889 ydcM b1432 CDS − 1502929 1504104 ydcO b1433 CDS + 1504196 1504732 ydcN b1434 CDS + 1504805 1506766 ydcP b1435 CDS − 1506858 1507088 yncJ b1436 CDS − 1507274 1507471 b1437 CDS + 1507511 1507948 ydcQ b1438 CDS + 1508027 1509433 ydcR b1439 CDS + 1509678 1510823 ydcS b1440 CDS + 1510841 1511854 ydcT b1441 CDS + 1511855 1512796 ydcU b1442 CDS + 1512786 1513580 ydcV b1443 CDS + 1513602 1515026 ydcW b1444 CDS + 1515413 1515586 ydcX b1445 CDS + 1515672 1515905 ydcY b1446 CDS − 1515906 1516355 ydcZ b1447 CDS − 1516352 1516870 yncA b1448 CDS + 1517051 1518088 yncB b1449 CDS + 1518286 1518951 yncC b1450 CDS − 1518987 1521089 yncD b1451 CDS + 1521331 1522392 yncE b1452 CDS − 1522505 1524004 ansP b1453 CDS + 1524271 1524888 yncG b1454 CDS + 1524964 1525176 yncH b1455 CDS + 1525914 1527962 rhsE b1456 CDS + 1527946 1528428 ydcD b1457 CDS + 1528610 1529356 b1458 CDS + 1529400 1529600 b1459 CDS + 1529840 1530976 ydcC b1460 CDS + 1531076 1531309 ydcE b1461 CDS − 1531306 1531875 yddH b1462 CDS + 1532048 1532893 nhoA b1463 CDS − 1532989 1533882 yddE b1464 CDS − 1533961 1534641 narV b1465 CDS − 1534638 1535333 narW b1466 CDS − 1535333 1536877 narY b1467 CDS − 1536874 1540614 narZ b1468 CDS − 1540696 1542084 narU b1469 CDS − 1542408 1542743 yddJ b1470 CDS − 1542782 1543738 yddK b1471 CDS − 1543762 1544052 b1472 CDS − 1544312 1545136 yddG b1473 CDS + 1545425 1548472 fdnG b1474 CDS + 1548485 1549369 fdnH b1475 CDS + 1549362 1550015 fdnI b1476 CDS − 1550422 1550784 yddM b1477 CDS − 1550852 1551862 adhP b1478 CDS − 1551996 1553693 sfcA b1479 CDS − 1553850 1553987 rpsV b1480 CDS − 1554089 1554304 bdm b1481 CDS + 1554649 1555080 osmC b1482 CDS − 1555136 1556062 yddO b1483 CDS − 1556055 1557041 yddP b1484 CDS − 1557038 1557934 yddQ b1485 CDS − 1557931 1558953 yddR b1486 CDS − 1558955 1560505 yddS b1487 CDS − 1560519 1561100 ddpX b1488 CDS − 1561358 1563757 dos b1489 CDS − 1563782 1564825 yddV b1490 CDS − 1565528 1566847 yddW b1491 CDS − 1566978 1568513 xasA b1492 CDS − 1568669 1570069 gadB b1493 CDS − 1570431 1573226 pqqL b1494 CDS − 1573271 1575643 yddB b1495 CDS − 1575681 1577366 yddA b1496 CDS − 1577657 1578814 ydeM b1497 CDS − 1578866 1580548 ydeN b1498 CDS − 1580950 1581711 ydeO b1499 CDS − 1581786 1581983 b1500 CDS − 1582231 1584510 ydeP b1501 CDS − 1584844 1585758 ydeQ b1502 CDS − 1585817 1586320 ydeR b1503 CDS − 1586333 1586863 ydeS b1504 CDS − 1586877 1588025 ydeT b1505 CDS − 1588381 1588560 b1506 CDS − 1588878 1590200 hipA b1507 CDS − 1590200 1590466 hipB b1508 CDS − 1590689 1592089 ydeU b1509 CDS − 1592133 1596110 ydeK b1510 CDS − 1596641 1598233 ydeV b1511 CDS − 1598312 1599265 ydeW b1512 CDS + 1599514 1601049 ego b1513 CDS + 1601043 1602071 ydeY b1514 CDS + 1602071 1603063 ydeZ b1515 CDS + 1603075 1604097 yneA b1516 CDS + 1604124 1604999 yneB b1517 CDS + 1605023 1605313 yneC b1518 CDS + 1605370 1606128 tam b1519 CDS − 1606132 1607046 yneE b1520 CDS − 1607253 1608704 uxaB b1521 CDS − 1608931 1609878 yneF b1522 CDS − 1609990 1610349 yneG b1523 CDS − 1610349 1611275 yneH b1524 CDS − 1611339 1612727 yneI b1525 CDS + 1612828 1613709 yneJ b1526 CDS + 1613787 1614902 yneK b1527 CDS + 1615052 1616242 sotB b1528 CDS − 1616267 1616932 marC b1529 CDS + 1617144 1617578 marR b1530 CDS + 1617598 1617981 marA b1531 CDS + 1618013 1618231 marB b1532 CDS − 1618262 1619161 eamA b1533 CDS + 1619356 1620543 ydeE b1534 CDS − 1620984 1621874 ydeH b1535 CDS − 1622129 1622521 ydeI b1536 CDS + 1622797 1623315 ydeJ b1537 CDS − 1623359 1625404 dcp b1538 CDS + 1625541 1626287 ydfG b1539 CDS + 1626376 1627062 ydfH b1540 CDS + 1627239 1627442 ydfZ b1541 CDS − 1627477 1628937 ydfI b1542 CDS − 1629026 1630309 ydfJ b1543 CDS + 1631063 1631329 ydfK b1544 CDS + 1631646 1632236 pinQ b1545 CDS − 1632334 1632909 tfaQ b1546 CDS − 1632909 1633871 stfQ b1547 CDS − 1633822 1634391 nohA b1548 CDS + 1635071 1635481 ydfO b1549 CDS − 1635633 1635806 gnsB b1550 CDS − 1635978 1636133 ynfN b1551 CDS − 1636479 1636691 cspI b1552 CDS − 1637054 1637551 ydfP b1553 CDS − 1637548 1638081 ydfQ b1554 CDS − 1638078 1638389 ydfR b1555 CDS − 1638394 1638609 essQ b1556 CDS − 1639363 1639578 cspB b1557 CDS + 1639879 1640091 cspF b1558 CDS − 1640513 1641265 ydfT b1559 CDS − 1641279 1642328 ydfU b1560 CDS − 1642675 1642926 rem b1561 CDS − 1643143 1643298 hokD b1562 CDS − 1643370 1643657 relE b1563 CDS − 1643657 1643896 relB b1564 CDS + 1643921 1644226 ydfV b1565 CDS + 1644429 1644761 flxA b1566 CDS − 1645198 1645347 ydfW b1567 CDS − 1645370 1645660 ydfX b1568 CDS − 1645644 1645874 dicC b1569 CDS + 1645958 1646365 dicA b1570 CDS + 1646532 1646687 ydfA b1571 CDS + 1646689 1646817 ydfB b1572 CDS + 1646847 1647065 ydfC b1573 misc_RNA + 1647406 1647458 dicF b1574 CDS + 1647633 1647821 dicB b1575 CDS + 1647818 1648009 ydfD b1576 CDS + 1648102 1649022 ydfE b1577 repeat_region − 1648867 1649572 CDS + 1648905 1649561 b1578 CDS + 1649536 1650732 intQ b1579 CDS − 1650920 1651939 rspB b1580 CDS − 1651951 1653165 rspA b1581 CDS − 1653371 1653697 ynfA b1582 CDS + 1653832 1654173 ynfB b1583 CDS + 1654208 1654768 speG b1584 CDS − 1654771 1655481 ynfC b1585 CDS + 1655589 1655894 ynfD b1586 CDS + 1656093 1658519 ynfE b1587 CDS + 1658580 1661003 ynfF b1588 CDS + 1661014 1661631 ynfG b1589 CDS + 1661633 1662487 ynfH b1590 CDS + 1662530 1663144 ynfI b1591 CDS + 1663339 1664595 ynfJ b1592 CDS − 1664548 1665243 ynfK b1593 CDS − 1665368 1666588 mlc b1594 CDS − 1666723 1667616 ynfL b1595 CDS + 1667723 1668976 ynfM b1596 CDS + 1669400 1669708 asr b1597 CDS + 1669984 1670805 ydgD b1598 CDS − 1670844 1671173 ydgE b1599 CDS − 1671160 1671525 ydgF b1600 CDS + 1671937 1672971 ydgG b1601 CDS − 1672996 1674384 pntB b1602 CDS − 1674395 1675927 pntA b1603 CDS + 1676451 1677395 ydgH b1604 CDS + 1677581 1678963 ydgI b1605 CDS + 1679000 1679722 ydgB b1606 CDS − 1679719 1680054 ydgC b1607 CDS + 1680174 1680902 rstA b1608 CDS + 1680906 1682207 rstB b1609 CDS + 1682283 1683212 tus b1610 CDS − 1683209 1684612 fumC b1611 CDS − 1684755 1686401 fumA b1612 CDS + 1686600 1687775 manA b1613 CDS + 1687876 1689384 ydgA b1614 CDS − 1689610 1690875 uidC b1615 CDS − 1690914 1692287 uidB b1616 CDS − 1692284 1694095 uidA b1617 CDS − 1694486 1695076 uidR b1618 CDS − 1695297 1696064 hdhA b1619 CDS − 1696176 1697204 malI b1620 CDS + 1697379 1698971 malX b1621 CDS + 1698981 1700153 malY b1622 CDS + 1700257 1701258 add b1623 CDS − 1701292 1702332 ydgJ b1624 CDS + 1702973 1703188 ydgT b1625 CDS + 1703274 1703714 ydgK b1626 CDS + 1703791 1704372 rnfA b1627 CDS + 1704372 1704950 rnfB b1628 CDS + 1704943 1707165 rnfC b1629 CDS + 1707166 1708224 rnfD b1630 CDS + 1708228 1708848 rnfG b1631 CDS + 1708852 1709547 rnfE b1632 CDS + 1709547 1710182 nth b1633 CDS + 1710793 1712295 ydgR b1634 CDS + 1712401 1713006 gst b1635 CDS − 1713050 1713913 pdxY b1636 CDS − 1713972 1715246 tyrS b1637 CDS − 1715375 1716031 pdxH b1638 CDS − 1716090 1716419 ydhA b1639 CDS − 1716517 1717626 ydhH b1640 CDS + 1717900 1718367 slyB b1641 CDS − 1718414 1718848 slyA b1642 CDS + 1719049 1719285 ydhI b1643 CDS + 1719288 1720145 ydhJ b1644 CDS + 1720145 1722157 ydhK b1645 CDS − 1722158 1722679 sodC b1646 CDS − 1722760 1723656 ydhF b1647 CDS − 1723705 1723944 ydhL b1648 CDS + 1724131 1724646 ydhM b1649 CDS + 1724683 1725780 nemA b1650 CDS + 1725861 1726268 gloA b1651 CDS + 1726371 1727018 rnt b1652 CDS + 1727111 1731727 lhr b1653 CDS − 1731778 1732125 ydhD b1654 CDS + 1732459 1733274 ydhO b1655 CDS + 1733402 1733983 sodB b1656 CDS − 1734145 1735314 ydhP b1657 CDS + 1735868 1736893 purR b1658 CDS − 1736890 1737822 ydhB b1659 CDS + 1737935 1739146 ydhC b1660 CDS + 1739437 1740585 cfa b1661 CDS − 1740625 1741266 ribC b1662 CDS + 1741481 1742854 norM b1663 CDS − 1742895 1744151 ydhQ b1664 tRNA + 1744459 1744535 valV b1665 tRNA + 1744540 1744616 valW b1666 CDS + 1744724 1745029 ydhR b1667 CDS + 1745155 1746759 ydhS b1668 CDS − 1746771 1747583 ydhT b1669 CDS − 1747587 1748372 ydhU b1670 CDS − 1748369 1749037 ydhX b1671 CDS − 1749101 1749748 ydhW b1672 CDS − 1749752 1751854 ydhV b1673 CDS − 1751875 1752501 ydhY b1674 CDS − 1752956 1753165 ydhZ b1675 CDS + 1753722 1755134 pykF b1676 CDS + 1755445 1755681 lpp b1677 CDS − 1755745 1756749 ynhG b1678 CDS − 1756898 1757314 sufE b1679 CDS − 1757327 1758547 sufS b1680 CDS − 1758544 1759815 sufD b1681 CDS − 1759790 1760536 sufC b1682 CDS − 1760546 1762033 sufB b1683 CDS − 1762042 1762410 sufA b1684 CDS − 1762958 1763227 ydiH b1685 CDS − 1763246 1763656 ydiI b1686 CDS − 1763653 1766709 ydiJ b1687 CDS + 1767098 1768210 ydiK b1688 CDS + 1768639 1768995 ydiL b1689 CDS + 1769095 1770309 ydiM b1690 CDS + 1770536 1771801 ydiN b1691 CDS + 1771813 1772679 ydiB b1692 CDS + 1772710 1773468 aroD b1693 CDS + 1773611 1775206 ydiF b1694 CDS + 1775220 1776371 ydiO b1695 CDS − 1776414 1777325 ydiP b1696 CDS + 1777641 1778405 ydiQ b1697 CDS + 1778425 1779363 ydiR b1698 CDS + 1779419 1780708 ydiS b1699 CDS + 1780705 1780998 ydiT b1700 CDS + 1781001 1782701 ydiD b1701 CDS − 1782758 1785136 ppsA b1702 CDS + 1785469 1786302 ydiA b1703 CDS + 1786459 1787505 aroH b1704 CDS + 1787637 1787828 ydiE b1705 CDS − 1787832 1789268 ydiU b1706 CDS − 1789331 1790044 ydiV b1707 CDS − 1790291 1790755 nlpC b1708 CDS − 1790833 1791582 btuD b1709 CDS − 1791582 1792133 btuE b1710 CDS − 1792196 1793176 btuC b1711 CDS − 1793277 1793576 himA b1712 CDS − 1793581 1795968 pheT b1713 CDS − 1795983 1796966 pheS b1714 CDS − 1797250 1797294 pheM b1715 CDS − 1797417 1797773 rplT b1716 CDS − 1797826 1798023 rpmI b1717 CDS − 1798120 1798662 infC b1718 CDS − 1798666 1800594 thrS b1719 CDS + 1801118 1801591 arpB_1 b1720 CDS + 1801602 1803017 arpB_2 b1721 CDS − 1803349 1804107 ydiY b1722 CDS + 1804394 1805323 pfkB b1723 CDS + 1805424 1805714 ydiZ b1724 CDS + 1805820 1806680 yniA b1725 CDS − 1806721 1807257 yniB b1726 CDS + 1807404 1808072 yniC b1727 CDS + 1808235 1808825 ydjM b1728 CDS + 1808958 1810349 ydjN b1729 CDS − 1810353 1811168 ydjO b1730 CDS − 1811445 1811708 cedA b1731 CDS + 1811891 1814152 katE b1732 CDS − 1814410 1815159 ydjC b1733 CDS − 1815172 1816524 celF b1734 CDS − 1816629 1817471 celD b1735 CDS − 1817479 1817829 celC b1736 CDS − 1817880 1819238 celB b1737 CDS − 1819323 1819643 celA b1738 CDS − 1819942 1820280 osmE b1739 CDS + 1820482 1821309 nadE b1740 CDS + 1821539 1822426 ydjQ b1741 CDS − 1822386 1822961 ydjR b1742 CDS − 1823164 1823649 spy b1743 CDS − 1823979 1824947 astE b1744 CDS − 1824940 1826283 astB b1745 CDS − 1826280 1827758 astD b1746 CDS − 1827755 1828789 astA b1747 CDS − 1828786 1830006 astC b1748 CDS + 1830452 1831258 xthA b1749 CDS + 1831425 1832135 ydjX b1750 CDS + 1832140 1832817 ydjY b1751 CDS + 1832832 1833539 ydjZ b1752 CDS + 1833539 1834087 ynjA b1753 CDS + 1834097 1835263 ynjB b1754 CDS + 1835281 1836771 ynjC b1755 CDS + 1836771 1837424 ynjD b1756 CDS + 1837491 1838798 ynjE b1757 CDS − 1838807 1839427 ynjF b1758 CDS + 1839514 1839921 nudG b1759 CDS − 1839887 1840159 ynjH b1760 CDS + 1840395 1841738 gdhA b1761 CDS − 1841855 1842895 ynjI b1762 CDS − 1843023 1844984 topB b1763 CDS − 1844989 1846032 selD b1764 CDS − 1846149 1846700 ydjA b1765 CDS + 1846861 1848717 sppA b1766 CDS + 1848884 1849900 ansA b1767 CDS + 1849911 1850552 pncA b1768 CDS − 1850645 1852003 ydjE b1769 CDS − 1852120 1852878 ydjF b1770 CDS − 1853015 1853995 ydjG b1771 CDS − 1854005 1854952 ydjH b1772 CDS − 1854957 1855793 ydjI b1773 CDS − 1855814 1856857 ydjJ b1774 CDS − 1856874 1858253 ydjK b1775 CDS − 1858280 1859356 ydjL b1776 CDS − 1859726 1859998 yeaC b1777 CDS − 1860040 1860453 yeaA b1778 CDS + 1860795 1861790 gapA b1779 CDS + 1861874 1862758 yeaD b1780 CDS − 1862806 1863660 yeaE b1781 CDS − 1863750 1864496 mipA b1782 CDS + 1864932 1866866 yeaG b1783 CDS + 1866979 1868262 yeaH b1784 CDS + 1868409 1869884 yeaI b1785 CDS + 1870065 1871555 yeaJ b1786 CDS + 1871598 1872101 yeaK b1787 CDS − 1872102 1872206 b1788 CDS + 1872376 1872822 yeaL b1789 CDS − 1872779 1873600 yeaM b1790 CDS + 1873697 1874878 yeaN b1791 CDS + 1874933 1875280 yeaO b1792 CDS − 1875302 1875556 yoaF b1793 CDS + 1875739 1876764 yeaP b1794 CDS − 1877031 1877279 yeaQ b1795 CDS − 1877427 1877609 yoaG b1796 CDS − 1877613 1877972 yeaR b1797 CDS − 1878145 1878783 yeaS b1798 CDS − 1878910 1879833 yeaT b1799 CDS + 1879936 1881021 yeaU b1800 CDS + 1881212 1882657 yeaV b1801 CDS + 1882689 1883813 yeaW b1802 CDS + 1883869 1884834 yeaX b1803 CDS − 1884888 1886015 rnd b1804 CDS − 1886085 1887770 fadD b1805 CDS − 1887975 1888556 yeaY b1806 CDS − 1888596 1889291 yeaZ b1807 CDS − 1889349 1891259 yoaA b1808 CDS + 1891391 1891735 yoaB b1809 CDS + 1892097 1892456 yoaC b1810 CDS − 1892576 1892755 yoaH b1811 CDS + 1892829 1894190 pabB b1812 CDS + 1894194 1894772 yeaB b1813 CDS + 1894956 1896320 sdaA b1814 CDS + 1896451 1898049 yoaD b1815 CDS − 1898053 1899609 yoaE b1816 CDS + 1900072 1901043 manX b1817 CDS + 1901106 1901906 manY b1818 CDS + 1901910 1902770 manZ b1819 CDS + 1902825 1903283 yobD b1820 CDS + 1903712 1904278 yebN b1821 CDS − 1904275 1905084 rrmA b1822 CDS − 1905250 1905459 cspC b1823 CDS − 1905472 1905615 yobF b1824 CDS − 1906285 1906572 yebO b1825 CDS − 1906647 1906790 yobG b1826 CDS − 1907332 1908123 kdgR b1827 CDS + 1908300 1909673 yebQ b1828 CDS − 1909719 1910600 htpX b1829 CDS − 1910792 1912840 prc b1830 CDS − 1912860 1913558 proQ b1831 CDS − 1913655 1914206 yebR b1832 CDS + 1914282 1915565 yebS b1833 CDS + 1915534 1918167 yebT b1834 CDS + 1918247 1919686 yebU b1835 CDS + 1919804 1920040 yebV b1836 CDS + 1920145 1920336 yebW b1837 CDS − 1920337 1920993 pphA b1838 CDS − 1921389 1921730 yebY b1839 CDS − 1921743 1922615 yebZ b1840 CDS − 1922619 1922993 yobA b1841 CDS + 1923132 1923362 holE b1842 CDS + 1923464 1924120 yobB b1843 CDS + 1924144 1924806 exoX b1844 CDS − 1924803 1926863 ptrB b1845 CDS − 1927072 1927731 yebE b1846 CDS − 1928058 1928426 yebF b1847 CDS − 1928481 1928771 yebG b1848 CDS + 1928905 1930083 purT b1849 CDS − 1930139 1930780 eda b1850 CDS − 1930817 1932628 edd b1851 CDS − 1932863 1934338 zwf b1852 CDS + 1934676 1935545 yebK b1853 CDS + 1935673 1937115 pykA b1854 CDS − 1937246 1938217 msbB b1855 CDS − 1938337 1939659 yebA b1856 CDS − 1939675 1940607 znuA b1857 CDS + 1940686 1941441 znuC b1858 CDS + 1941438 1942223 znuB b1859 CDS − 1942370 1943380 ruvB b1860 CDS − 1943389 1944000 ruvA b1861 CDS + 1944275 1944877 yebB b1862 CDS − 1944879 1945400 ruvC b1863 CDS − 1945435 1946175 yebC b1864 CDS − 1946204 1946656 ntpA b1865 CDS − 1946774 1948546 aspS b1866 CDS + 1948856 1949422 yecD b1867 CDS + 1949419 1950237 yecE b1868 CDS + 1950290 1950685 yecN b1869 CDS + 1950726 1951469 yecO b1870 CDS + 1951466 1952437 yecP b1871 CDS − 1952602 1955031 torZ b1872 CDS − 1955056 1956156 torY b1873 CDS − 1956544 1957290 cutC b1874 CDS − 1957304 1957870 yecM b1875 CDS + 1958086 1959819 argS b1876 CDS + 1959996 1960484 yecT b1877 CDS − 1960604 1960996 flhE b1878 CDS − 1960996 1963074 flhA b1879 CDS − 1963067 1964215 flhB b1880 CDS − 1964417 1965061 cheZ b1881 CDS − 1965072 1965461 cheY b1882 CDS − 1965476 1966525 cheB b1883 CDS − 1966528 1967388 cheR b1884 CDS − 1967407 1969008 tap b1885 CDS − 1969054 1970715 tar b1886 CDS − 1970860 1971363 cheW b1887 CDS − 1971384 1973348 cheA b1888 CDS − 1973353 1974279 motB b1889 CDS − 1974276 1975163 motA b1890 CDS − 1975290 1975868 flhC b1891 CDS − 1975871 1976230 flhD b1892 repeat_region − 1976527 1977294 CDS − 1976542 1977045 insB_5 b1893 CDS − 1976964 1977239 insA_5 b1894 CDS + 1977777 1978205 yecG b1895 CDS − 1978212 1979636 otsA b1896 CDS − 1979611 1980411 otsB b1897 CDS − 1981579 1983093 araG b1900 CDS − 1983163 1984152 araF b1901 CDS + 1984949 1985452 yecI b1902 CDS + 1985468 1985806 b1903 CDS + 1986246 1986569 yecR b1904 CDS + 1986740 1987237 ftn b1905 CDS − 1987275 1987514 yecH b1906 CDS + 1987705 1988916 tyrP b1907 CDS − 1988978 1989643 yecA b1908 tRNA − 1989839 1989925 leuZ b1909 tRNA − 1989938 1990011 cysT b1910 tRNA − 1990066 1990141 glyW b1911 CDS − 1990293 1990841 pgsA b1912 CDS − 1990898 1992730 uvrC b1913 CDS − 1992727 1993383 uvrY b1914 CDS + 1993842 1994066 yecF b1915 CDS − 1994134 1994856 sdiA b1916 CDS − 1995086 1995838 yecC b1917 CDS − 1995835 1996503 yecS b1918 CDS − 1996518 1997504 yedO b1919 CDS − 1997609 1998409 fliY b1920 CDS − 1998497 1999048 fliZ b1921 CDS − 1999094 1999813 fliA b1922 CDS − 2000134 2001630 fliC b1923 CDS + 2001896 2003302 fliD b1924 CDS + 2003327 2003737 fliS b1925 CDS + 2003737 2004102 fliT b1926 CDS + 2004180 2005667 amyA b1927 CDS − 2005701 2006114 yedD b1928 CDS + 2006301 2007506 yedE b1929 CDS + 2007503 2007736 yedF b1930 CDS + 2007845 2008513 yedK b1931 CDS + 2008624 2009103 yedL b1932 CDS − 2009372 2009563 yedN_2 b1933 CDS − 2009573 2009893 yedN_1 b1934 CDS − 2010025 2010375 yedM b1935 CDS + 2010526 2010804 b1936 CDS − 2010724 2011038 fliE b1937 CDS + 2011253 2012911 fliF b1938 CDS + 2012904 2013899 fliG b1939 CDS + 2013892 2014578 fliH b1940 CDS + 2014578 2015951 fliI b1941 CDS + 2015970 2016413 fliJ b1942 CDS + 2016410 2017537 fliK b1943 CDS + 2017642 2018106 fliL b1944 CDS + 2018111 2019115 fliM b1945 CDS + 2019112 2019525 fliN b1946 CDS + 2019528 2019893 fliO b1947 CDS + 2019893 2020630 fliP b1948 CDS + 2020640 2020909 fliQ b1949 CDS + 2020917 2021702 fliR b1950 CDS + 2021992 2022615 rcsA b1951 CDS − 2022659 2022847 dsrB b1952 CDS + 2023010 2023237 yodD b1953 misc_RNA − 2023251 2023337 dsrA b1954 CDS + 2023535 2024350 yedP b1955 CDS − 2024347 2026041 yedQ b1956 CDS − 2026212 2026394 yodC b1957 CDS − 2026473 2027390 yedI b1958 CDS + 2027563 2028483 yedA b1959 CDS − 2028472 2028942 vsr b1960 CDS − 2028923 2030341 dcm b1961 CDS − 2030408 2031103 yedJ b1962 CDS − 2031143 2031508 yedR b1963 CDS + 2032075 2032560 yedS_1 b1964 CDS + 2032570 2032779 yedS_2 b1965 CDS + 2032863 2033267 yedS_3 b1966 CDS + 2033859 2034710 yedU b1967 CDS − 2034818 2036176 yedV b1968 CDS − 2036176 2036847 yedW b1969 CDS + 2036980 2037393 yedX b1970 CDS + 2037502 2038506 yedY b1971 CDS + 2038507 2039142 yedZ b1972 CDS + 2039399 2040049 yodA b1973 CDS + 2040392 2040922 yodB b1974 tRNA − 2041492 2041581 serU b1975 CDS + 2041675 2042472 yeeI b1976 tRNA + 2042573 2042648 asnT b1977 CDS + 2042935 2050038 yeeJ b1978 CDS − 2050300 2050626 yeeL_2 b1979 CDS − 2050648 2051352 yeeL_1 b1980 CDS + 2051667 2052983 shiA b1981 CDS + 2053085 2054539 amn b1982 CDS + 2054882 2055598 yeeN b1983 tRNA − 2056051 2056126 asnW b1984 CDS − 2056227 2057870 yeeO b1985 tRNA + 2057875 2057950 asnU b1986 CDS − 2057988 2058938 cbl b1987 CDS − 2059040 2059957 nac b1988 tRNA + 2060284 2060359 asnV b1989 CDS − 2060415 2061347 erfK b1990 CDS − 2061412 2062491 cobT b1991 CDS − 2062503 2063246 cobS b1992 CDS − 2063243 2063788 cobU b1993 repeat_region + 2064183 2065377 CDS − 2064329 2065345 trs5_6 b1994 CDS + 2066659 2067051 b1995 repeat_egion + 2066965 2068295 CDS − 2066976 2067881 yi22_3 b1996 CDS − 2067839 2068249 yi21_3 b1997 CDS + 2068268 2068528 b1998 CDS + 2068810 2069235 yeeP b1999 CDS + 2069563 2072682 flu b2000 CDS + 2072803 2074335 yeeR b2001 CDS + 2074332 2074778 yeeS b2002 CDS + 2074841 2075062 yeeT b2003 CDS + 2075136 2075504 yeeU b2004 CDS + 2075593 2075967 yeeV b2005 CDS + 2075964 2076158 yeeW b2006 CDS − 2077056 2077451 yeeX b2007 CDS − 2077557 2078615 yeeA b2008 CDS − 2078813 2079286 sbmC b2009 CDS − 2079405 2080571 dacD b2010 CDS + 2080780 2082207 sbcB b2011 CDS − 2082250 2082477 yeeD b2012 CDS − 2082491 2083549 yeeE b2013 CDS − 2083728 2085086 yeeF b2014 CDS − 2085353 2086282 yeeY b2015 CDS − 2086328 2087152 yeeZ b2016 CDS − 2087486 2087737 yefM b2017 CDS + 2088020 2088070 hisL b2018 CDS + 2088216 2089115 hisG b2019 CDS + 2089121 2090425 hisD b2020 CDS + 2090422 2091492 hisC b2021 CDS + 2091492 2092559 hisB b2022 CDS + 2092559 2093149 hisH b2023 CDS + 2093149 2093886 hisA b2024 CDS + 2093868 2094644 hisF b2025 CDS + 2094638 2096549 hisI b2026 CDS − 2095345 2096325 cld b2027 CDS − 2096471 2097637 ugd b2028 CDS − 2097886 2099292 gnd b2029 repeat_region + 2099773 2100967 CDS − 2099919 2100935 trs5_7 b2030 CDS − 2099420 2101413 wbbL b2031 CDS − 2101415 2102533 wbbK b2032 CDS − 2102518 2103108 wbbJ b2033 CDS − 2103089 2104081 wbbI b2034 CDS − 2104084 2105250 rfc b2035 CDS − 2105250 2106353 glf b2036 CDS − 2106361 2107608 rfbX b2037 CDS − 2107605 2108162 rfbC b2038 CDS − 2108162 2109043 rfbA b2039 CDS − 2109101 2110000 rfbD b2040 CDS − 2110000 2111085 rfbB b2041 CDS − 2111458 2112351 galF b2042 CDS − 2112526 2113920 wcaM b2043 CDS − 2113931 2115151 wcaL b2044 CDS − 2115148 2116428 wcaK b2045 CDS − 2116704 2118182 wzxC b2046 CDS − 2118184 2119578 wcaJ b2047 CDS − 2119633 2121003 cpsG b2048 CDS − 2121108 2122544 cpsB b2049 CDS − 2122547 2123770 wcaI b2050 CDS − 2123767 2124246 wcaH b2051 CDS − 2124249 2125214 wcaG b2052 CDS − 2125217 2126338 gmd b2053 CDS − 2126364 2126912 wcaF b2054 CDS − 2126928 2127674 wcaE b2055 CDS − 2127685 2128902 wcaD b2056 CDS − 2128877 2130094 wcaC b2057 CDS − 2130091 2130579 wcaB b2058 CDS − 2130582 2131421 wcaA b2059 CDS − 2131514 2133676 wzc b2060 CDS − 2133679 2134122 wzb b2061 CDS − 2134128 2135267 wza b2062 CDS + 2135926 2137509 yegH b2063 CDS − 2137783 2139636 asmA b2064 CDS − 2139658 2140239 dcd b2065 CDS − 2140331 2140972 udk b2066 CDS + 2141290 2144607 yegE b2067 CDS − 2144716 2145564 alkA b2068 CDS + 2145698 2147050 yegD b2069 CDS − 2147063 2149009 yegI b2070 CDS + 2149209 2149670 yegJ b2071 CDS − 2149735 2150496 yegK b2072 CDS − 2150493 2151152 yegL b2073 CDS + 2152040 2153287 yegM b2074 CDS + 2153287 2156409 yegN b2075 CDS + 2156410 2159487 yegO b2076 CDS + 2159488 2160903 yegB b2077 CDS + 2160900 2162303 baeS b2078 CDS + 2162300 2163022 baeR b2079 CDS + 2163213 2163545 yegP b2080 CDS + 2163692 2165053 yegQ b2081 CDS − 2165326 2165544 ogrK b2082 CDS − 2165626 2165844 yegZ b2083 CDS − 2165759 2166025 b2084 CDS − 2166013 2166330 yegR b2085 CDS + 2166736 2167635 yegS b2086 CDS − 2167717 2168163 gatR_1 b2087 repeat_region + 2168195 2169452 CDS + 2168251 2168559 b2088 CDS + 2168556 2169422 tra5_4 b2089 CDS − 2169419 2169757 gatR_2 b2090 CDS − 2169857 2170897 gatD b2091 CDS − 2170945 2172300 gatC b2092 CDS − 2172304 2172588 gatB b2093 CDS − 2172619 2173071 gatA b2094 CDS − 2173081 2174343 gatZ b2095 CDS − 2174372 2175226 gatY b2096 CDS − 2175534 2176586 fbaB b2097 CDS + 2176843 2178120 yegT b2098 CDS + 2178117 2179121 yegU b2099 CDS + 2179118 2180083 yegV b2100 CDS − 2180057 2180803 yegW b2101 CDS − 2180855 2181673 yegX b2102 CDS − 2181738 2182538 thiD b2103 CDS − 2182535 2183323 thiM b2104 CDS − 2183546 2183818 yohL b2105 CDS + 2183939 2184763 yohM b2106 CDS + 2184982 2185320 yohN b2107 CDS − 2185402 2186436 yehA b2108 CDS − 2186452 2188932 yehB b2109 CDS − 2188948 2189667 yehC b2110 CDS − 2189702 2190244 yehD b2111 CDS − 2190537 2190818 yehE b2112 CDS − 2191081 2192190 mrp b2113 CDS + 2192322 2194355 metG b2114 CDS + 2194496 2195320 molR_1 b2115 CDS + 2195432 2197369 molR_2 b2116 CDS + 2197344 2198291 molR_3 b2117 CDS + 2198301 2201933 yehI b2118 CDS + 2202618 2203706 yehL b2119 CDS + 2203717 2205996 yehM b2120 CDS + 2205989 2207125 yehP b2121 CDS + 2207122 2208966 yehQ b2122 CDS + 2209247 2209708 yehR b2123 CDS − 2209748 2210218 yehS b2124 CDS − 2210265 2210984 yehT b2125 CDS − 2210981 2212666 yehU b2126 CDS + 2212888 2213619 yehV b2127 CDS − 2213767 2214498 yehW b2128 CDS − 2214503 2215429 yehX b2129 CDS − 2215422 2216579 yehY b2130 CDS − 2216586 2217503 yehZ b2131 CDS − 2217714 2220011 bglX b2132 CDS + 2220207 2221922 dld b2133 CDS − 2221960 2222892 pbpG b2134 CDS − 2223066 2223653 yohC b2135 CDS + 2223823 2224401 yohD b2136 CDS − 2224531 2225292 yohF b2137 CDS − 2225345 2226433 yohG b2138 CDS − 2226571 2226708 yohH b2139 CDS − 2227460 2228407 yohI b2140 CDS + 2228646 2229044 yohJ b2141 CDS + 2229041 2229736 yohK b2142 CDS + 2229866 2230750 cdd b2143 CDS + 2230900 2231619 sanA b2144 CDS + 2231622 2231861 yeiS b2145 CDS + 2232055 2233293 yeiT b2146 CDS + 2233287 2234522 yeiA b2147 CDS − 2234765 2235775 mglC b2148 CDS − 2235791 2237311 mglA b2149 CDS − 2237372 2238370 mglB b2150 CDS − 2238650 2239690 galS b2151 CDS − 2239832 2240989 yeiB b2152 CDS − 2241006 2241674 folE b2153 CDS + 2241932 2242768 yeiG b2154 CDS − 2242800 2244791 cirA b2155 CDS − 2245085 2246554 lysP b2156 CDS − 2246759 2247640 yeiE b2157 CDS + 2247739 2248788 yeiH b2158 CDS + 2248862 2249719 nfo b2159 CDS + 2249722 2250810 yeiI b2160 CDS − 2250917 2252167 yeiJ b2161 CDS − 2252267 2253208 rihB b2162 CDS + 2253377 2254036 yeiL b2163 CDS − 2254107 2255357 yeiM b2164 CDS − 2255451 2256389 yeiN b2165 CDS − 2256377 2257318 yeiC b2166 CDS − 2257741 2259432 fruA b2167 CDS − 2259449 2260387 fruK b2168 CDS − 2260387 2261517 fruB b2169 CDS + 2261885 2263066 setB b2170 CDS + 2263472 2264044 yeiP b2171 CDS + 2264267 2265733 yeiQ b2172 CDS + 2265851 2266837 yeiR b2173 CDS + 2266876 2267589 yeiU b2174 CDS + 2268001 2268567 spr b2175 CDS + 2268748 2270304 rtn b2176 CDS + 2270386 2272200 yejA b2177 CDS + 2272201 2273295 yejB b2178 CDS + 2273295 2274320 yejE b2179 CDS + 2274322 2275911 yejF b2180 CDS − 2275915 2276259 yejG b2181 CDS − 2276592 2277782 bcr b2182 CDS − 2277810 2278505 rsuA b2183 CDS + 2278654 2280414 yejH b2184 CDS + 2280539 2280823 rplY b2185 CDS − 2280962 2281969 yejK b2186 CDS + 2282151 2282378 yejL b2187 CDS + 2282398 2284158 yejM b2188 tRNA + 2284233 2284309 proL b2189 CDS − 2284412 2286922 yejO b2190 CDS + 2286927 2287049 b2191 repeat_region + 2286941 2288135 CDS − 2287087 2288103 trs5_8 b2192 CDS + 2288522 2289169 narP b2193 CDS − 2289380 2290432 ccmH b2194 CDS − 2290429 2290986 ccmG b2195 CDS − 2290983 2292926 ccmF b2196 CDS − 2292923 2293402 ccmE b2197 CDS − 2293399 2293608 ccmD b2198 CDS − 2293605 2294342 ccmC b2199 CDS − 2294384 2295046 ccmB b2200 CDS − 2295043 2295666 ccmA b2201 CDS − 2295679 2296281 napC b2202 CDS − 2296291 2296740 napB b2203 CDS − 2296737 2297600 napH b2204 CDS − 2297587 2298282 napG b2205 CDS − 2298289 2300775 napA b2206 CDS − 2300772 2301035 napD b2207 CDS − 2301025 2301519 napF b2208 CDS + 2301927 2302415 eco b2209 CDS − 2303130 2304776 mqo b2210 CDS − 2304994 2306637 yojI b2211 CDS − 2306713 2307363 alkB b2212 CDS − 2307363 2308427 ada b2213 CDS − 2308501 2309556 yojL b2214 CDS − 2309668 2310771 ompC b2215 CDS + 2311510 2314182 yojN b2216 CDS + 2314199 2314849 rcsB b2217 CDS − 2315049 2317850 rcsC b2218 CDS + 2318065 2319891 atoS b2219 CDS + 2319888 2321273 atoC b2220 CDS + 2321469 2322131 atoD b2221 CDS + 2322131 2322781 atoA b2222 CDS + 2322778 2324100 atoE b2223 CDS + 2324131 2325315 atoB b2224 CDS − 2325389 2326165 yfaP b2225 CDS − 2326170 2327819 yfaQ b2226 CDS − 2327820 2328305 yfaS_2 b2227 CDS − 2328321 2332424 yfaS_1 b2228 CDS − 2332358 2332981 yfaT b2229 CDS − 2332978 2334666 yfaA b2230 CDS − 2334815 2337442 gyrA b2231 CDS + 2337589 2338311 ubiG b2232 CDS − 2338439 2342191 yfaL b2233 CDS + 2342887 2345172 nrdA b2234 CDS + 2345406 2346536 nrdB b2235 CDS + 2346536 2346790 yfaE b2236 CDS − 2346844 2347494 inaA b2237 CDS + 2347709 2347915 yfaH b2238 CDS − 2347957 2349033 glpQ b2239 CDS − 2349038 2350396 glpT b2240 CDS + 2350669 2352297 glpA b2241 CDS + 2352287 2353546 glpB b2242 CDS + 2353543 2354733 glpC b2243 CDS + 2354926 2355825 yfaD b2244 CDS − 2356064 2356867 b2245 CDS − 2356885 2358174 yfaV b2246 CDS − 2358231 2359448 yfaW b2247 CDS − 2359451 2360233 yfaX b2248 CDS − 2360453 2361655 b2249 CDS − 2361755 2362297 yfaZ b2250 CDS + 2362576 2363001 yfaO b2251 CDS − 2363040 2363642 ais b2252 CDS + 2363932 2365089 yfbE b2253 CDS + 2365093 2366061 yfbF b2254 CDS + 2366061 2368043 yfbG b2255 CDS + 2368040 2368930 b2256 CDS + 2368930 2370582 arnT b2257 CDS + 2370914 2371300 yfbJ b2258 CDS − 2371294 2371560 pmrD b2259 CDS − 2371670 2373025 menE b2260 CDS − 2373022 2373984 menC b2261 CDS − 2373984 2374841 menB b2262 CDS − 2374856 2375614 yfbB b2263 CDS − 2375611 2377281 menD b2264 CDS − 2377370 2378665 menF b2265 CDS − 2378744 2379049 elaB b2266 CDS − 2379104 2379565 elaA b2267 CDS + 2379630 2380547 elaC b2268 CDS + 2380735 2381946 elaD b2269 CDS − 2382017 2383744 yfbK b2270 CDS + 2383882 2384853 yfbL b2271 CDS + 2384956 2385459 yfbM b2272 CDS − 2385732 2386448 yfbN b2273 CDS + 2386603 2387079 b2274 CDS + 2387135 2387986 b2275 CDS − 2388070 2389347 nuoN b2276 CDS − 2389534 2391063 nuoM b2277 CDS − 2391227 2393068 nuoL b2278 CDS − 2393065 2393367 nuoK b2279 CDS − 2393364 2393918 nuoJ b2280 CDS − 2393930 2394472 nuoI b2281 CDS − 2394487 2395464 nuoH b2282 CDS − 2395461 2398193 nuoG b2283 CDS − 2398240 2399577 nuoF b2284 CDS − 2399574 2400074 nuoE b2285 CDS − 2400077 2401879 nuoC b2286 CDS − 2401973 2402635 nuoB b2287 CDS − 2402651 2403094 nuoA b2288 CDS − 2403725 2404663 lrhA b2289 CDS + 2405583 2406800 yfbQ b2290 CDS + 2406884 2407483 b2291 CDS − 2407542 2409374 yfbS b2292 CDS − 2409461 2410111 yfbT b2293 CDS − 2410122 2410616 yfbU b2294 CDS − 2410699 2411154 b2295 CDS + 2411492 2412694 ackA b2296 CDS + 2412769 2414913 pta b2297 CDS + 2415103 2416623 yfcC b2298 CDS − 2416656 2417198 b2299 CDS − 2417256 2417810 yfcE b2300 CDS − 2417863 2418507 yfcF b2301 CDS + 2418643 2419290 yfcG b2302 CDS + 2419347 2419709 folX b2303 CDS + 2419730 2420623 yfcH b2304 CDS − 2420671 2421561 yfcI b2305 CDS − 2421758 2422531 hisP b2306 CDS − 2422539 2423255 hisM b2307 CDS − 2423252 2423938 hisQ b2308 CDS − 2424028 2424810 hisJ b2309 CDS − 2425031 2425813 argT b2310 CDS − 2426079 2426648 ubiX b2311 CDS − 2426743 2428260 purF b2312 CDS − 2428297 2428785 cvpA b2313 CDS − 2429044 2429706 dedD b2314 CDS − 2429696 2430964 folC b2315 CDS − 2431034 2431948 accD b2316 CDS − 2432104 2432763 dedA b2317 CDS − 2432846 2433658 truA b2318 CDS − 2433658 2434671 usg b2319 CDS − 2434737 2435873 pdxB b2320 CDS + 2435972 2436967 flk b2321 CDS − 2436964 2438142 b2322 CDS − 2438407 2439627 fabB b2323 CDS + 2439786 2441792 b2324 CDS − 2441913 2442191 yfcL b2325 CDS − 2442225 2442773 yfcM b2326 CDS − 2442773 2443582 yfcA b2327 CDS − 2443582 2444406 mepA b2328 CDS − 2444410 2445495 aroC b2329 CDS − 2445530 2446462 yfcB b2330 CDS + 2446628 2447179 yfcN b2331 CDS − 2447250 2448071 b2332 CDS − 2448073 2448612 yfcP b2333 CDS − 2448609 2449097 b2334 CDS − 2449094 2449606 b2335 CDS − 2449606 2450358 yfcS b2336 CDS − 2450378 2451274 b2337 CDS − 2451287 2453023 yfcU b2338 CDS − 2453105 2453668 b2339 CDS − 2454349 2454834 sixA b2340 CDS − 2455037 2457181 yfcX b2341 CDS − 2457181 2458491 yfcY b2342 CDS − 2458672 2458956 yfcZ b2343 CDS + 2459322 2460668 fadL b2344 CDS + 2461034 2462092 b2345 CDS − 2462274 2463029 vacJ b2346 CDS + 2463323 2464255 yfdC b2347 tRNA + 2464331 2464405 argW b2348 CDS + 2464567 2465724 intS b2349 CDS + 2465877 2466239 yfdG b2350 CDS + 2466236 2467156 yfdH b2351 CDS + 2467153 2468484 yfdI b2352 CDS + 2468825 2469127 tfaS b2353 CDS − 2469099 2469539 yfdK b2354 CDS − 2469566 2470084 yfdL b2355 CDS − 2470134 2470409 yfdM b2356 CDS − 2470409 2470903 yfdN b2357 CDS − 2470900 2471268 yfdO b2358 CDS + 2471542 2471988 yfdP b2359 CDS + 2472054 2472878 yfdQ b2360 CDS + 2473006 2473542 yfdR b2361 CDS + 2473533 2473895 yfdS b2362 CDS + 2473895 2474200 yfdT b2363 CDS − 2474716 2475651 dsdC b2364 CDS + 2475869 2477206 dsdX b2365 CDS + 2477224 2478552 dsdA b2366 CDS − 2478660 2480198 emrY b2367 CDS − 2480198 2481361 emrK b2368 CDS + 2481777 2482391 evgA b2369 CDS + 2482396 2485989 evgS b2370 CDS − 2486045 2487190 yfdE b2371 CDS − 2487264 2488208 yfdV b2372 CDS − 2488278 2489972 yfdU b2373 CDS − 2490026 2491276 yfdW b2374 CDS − 2491789 2492424 yfdX b2375 CDS + 2492720 2492995 ypdI b2376 CDS − 2493072 2493314 yfdY b2377 CDS + 2493667 2494587 ddg b2378 CDS − 2495079 2496317 yfdZ b2379 CDS + 2496693 2498390 ypdA b2380 CDS + 2498405 2499139 ypdB b2381 CDS + 2499152 2500009 ypdC b2382 CDS − 2500012 2502507 ypdD b2383 CDS − 2502532 2503569 ypdE b2384 CDS − 2503569 2504654 ypdF b2385 CDS − 2504669 2505916 ypdG b2386 CDS − 2505938 2506264 ypdH b2387 CDS − 2506483 2507448 glk b2388 CDS + 2507652 2508908 yfeO b2389 CDS + 2509023 2509349 ypeC b2390 CDS − 2509490 2510728 mntH b2392 CDS + 2511064 2512266 nupC b2393 repeat_region + 2512296 2513638 CDS + 2512353 2513465 yi81_3 b2394 CDS − 2513665 2515854 yfeA b2395 tRNA − 2516063 2516138 alaX b2396 tRNA − 2516178 2516253 alaW b2397 CDS + 2516489 2516833 yfeC b2398 CDS + 2516835 2517227 yfeD b2399 CDS − 2517279 2518694 gltX b2400 tRNA + 2518953 2519028 valU b2401 tRNA + 2519073 2519148 valX b2402 tRNA + 2519195 2519270 valY b2403 tRNA + 2519275 2519350 lysV b2404 CDS − 2519615 2520499 xapR b2405 CDS − 2520751 2522007 xapB b2406 CDS − 2522067 2522900 xapA b2407 CDS + 2523149 2523913 yfeN b2408 CDS − 2523952 2524878 yfeR b2409 CDS + 2524968 2525966 yfeH b2410 CDS − 2526183 2528198 ligA b2411 CDS − 2528269 2529255 zipA b2412 CDS + 2529485 2530246 cysZ b2413 CDS + 2530431 2531402 cysK b2414 CDS + 2531786 2532043 ptsH b2415 CDS + 2532088 2533815 ptsI b2416 CDS + 2533856 2534365 crr b2417 CDS − 2534408 2535259 pdxK b2418 CDS + 2535364 2535738 yfeK b2419 CDS + 2535771 2536505 yfeS b2420 CDS − 2536694 2537605 cysM b2421 CDS − 2537739 2538836 cysA b2422 CDS − 2538826 2539701 cysW b2423 CDS − 2539701 2540534 cysU b2424 CDS − 2540534 2541550 cysP b2425 CDS − 2541854 2542645 ucpA b2426 CDS − 2542774 2543631 yfeT b2427 CDS + 2543795 2544691 yfeU b2428 CDS + 2544695 2546119 yfeV b2429 CDS + 2546124 2547428 yfeW b2430 CDS − 2547668 2548567 yfeX b2431 CDS − 2548663 2549238 yfeY b2432 CDS − 2549299 2549748 yfeZ b2433 CDS − 2549735 2550160 ypeA b2434 CDS + 2550374 2551243 amiA b2435 CDS + 2551247 2552146 hemF b2436 CDS − 2552152 2553204 yfeG b2437 CDS − 2553250 2553750 yffI b2438 CDS − 2553763 2554422 eutL b2439 CDS − 2554432 2555319 eutC b2440 CDS − 2555340 2556701 eutB b2441 CDS + 2556793 2558088 intZ b2442 CDS + 2558279 2558920 b2443 CDS + 2559390 2559635 b2444 CDS + 2559632 2560015 yffN b2445 CDS + 2560133 2560549 yffO b2446 CDS + 2560546 2561139 b2447 CDS + 2561599 2561991 yffQ b2448 CDS + 2562002 2562394 b2449 CDS + 2562515 2563354 yffS b2450 CDS − 2563503 2564906 eutA b2451 CDS − 2564903 2556129 eutH b2452 CDS − 2566346 2567533 eutG b2453 CDS − 2567523 2568359 eutJ b2454 CDS − 2568370 2569773 eutE b2455 CDS − 2569785 2570072 cchB b2456 CDS − 2570179 2570472 cchA b2457 CDS − 2570511 2571527 eutI b2458 CDS − 2571524 2572327 eutT b2459 CDS − 2572324 2573025 eutQ b2460 CDS − 2573000 2573479 eutP b2461 CDS − 2573492 2573827 ypfE b2462 CDS − 2574120 2576399 maeB b2463 CDS + 2576688 2577638 talA b2464 CDS + 2577658 2579661 tktB b2465 CDS − 2579756 2580799 ypfG b2466 CDS − 2580925 2581500 yffH b2467 CDS − 2581568 2583547 aegA b2468 CDS + 2583753 2585453 narQ b2469 CDS + 2585617 2588730 acrD b2470 CDS + 2589269 2589625 yffB b2471 CDS + 2589629 2590756 dapE b2472 CDS − 2591094 2591792 ypfH b2473 CDS − 2591866 2593881 ypfI b2474 CDS − 2593896 2594759 b2475 CDS − 2594927 2595640 purC b2476 CDS − 2595853 2596887 nlpB b2477 CDS − 2596904 2597782 dapA b2478 CDS + 2597928 2598500 gcvR b2479 CDS + 2598500 2598970 bcp b2480 CDS + 2599223 2599840 hyfA b2481 CDS + 2599840 2601858 hyfB b2482 CDS + 2601869 2602816 hyfC b2483 CDS + 2602833 2604272 hyfD b2484 CDS + 2604284 2604934 hyfE b2485 CDS + 2604939 2606519 hyfF b2486 CDS + 2606509 2608176 hyfG b2487 CDS + 2608186 2608731 hyfH b2488 CDS + 2608728 2609486 hyfI b2489 CDS + 2609479 2609892 hyfJ b2490 CDS + 2609922 2611934 hyfR b2491 CDS + 2611956 2612804 focB b2492 CDS − 2612842 2613903 perM b2493 CDS + 2614116 2615579 b2494 CDS + 2615600 2615959 yfgD b2495 CDS − 2616097 2616843 hda b2496 CDS − 2616893 2618182 uraA b2497 CDS − 2618268 2618894 upp b2498 CDS + 2619219 2620256 purM b2499 CDS + 2620256 2620894 purN b2500 CDS + 2621066 2623132 ppk b2501 CDS + 2623137 2624678 ppx b2502 CDS − 2624717 2626960 yfgF b2503 CDS + 2627312 2627503 yfgG b2504 CDS + 2627814 2628332 yfgH b2505 CDS + 2628348 2628887 yfgI b2506 CDS − 2628980 2630557 guaA b2507 CDS − 2630626 2632092 guaB b2508 CDS + 2632254 2633624 xseA b2509 CDS − 2633621 2633836 yfgJ b2510 CDS − 2633906 2635378 yfgK b2511 CDS − 2635496 2636674 yfgL b2512 CDS − 2636685 2637305 yfgM b2513 CDS − 2637323 2638597 hisS b2514 CDS − 2638708 2639826 ispG b2515 CDS − 2639853 2640866 yfgA b2516 CDS − 2641151 2642305 yfgB b2517 CDS − 2642455 2642886 ndk b2518 CDS − 2643035 2645347 pbpC b2519 CDS − 2645348 2650309 yfhM b2520 CDS + 2650516 2651361 sseA b2521 CDS − 2652179 2652955 sseB b2522 CDS − 2653097 2654380 pepB b2523 CDS − 2654558 2654758 yfhJ b2524 CDS − 2654770 2655105 fdx b2525 CDS − 2655107 2656957 hscA b2526 CDS − 2656974 2657489 hscB b2527 CDS − 2657585 2657908 iscA b2528 CDS − 2657925 2658311 iscU b2529 CDS − 2658339 2659553 iscS b2530 CDS − 2659665 2660153 iscR b2531 CDS − 2660605 2661345 yfhQ b2532 CDS + 2661464 2662267 suhB b2533 CDS + 2662385 2663266 yfhR b2534 CDS + 2663457 2664737 csiE b2535 CDS − 2664729 2665868 hcaT b2536 CDS − 2666028 2666918 hcaR b2537 CDS + 2667054 2668415 hcaE b2538 CDS + 2668412 2668930 hcaF b2539 CDS + 2668930 2669250 hcaC b2540 CDS + 2669247 2670059 hcaB b2541 CDS + 2670069 2671271 hcaD b2542 CDS + 2671296 2671790 yphA b2543 CDS − 2671838 2672710 yphB b2544 CDS − 2672722 2673783 yphC b2545 CDS − 2673849 2674847 yphD b2546 CDS − 2674872 2676383 yphE b2547 CDS − 2676406 2677389 yphF b2548 CDS − 2677486 2680767 yphG b2549 CDS + 2680885 2682078 yphH b2550 CDS − 2682276 2683529 glyA b2551 CDS + 2683857 2685047 hmpA b2552 CDS − 2685092 2685430 glnB b2553 CDS − 2685491 2686825 yfhA b2554 CDS − 2686815 2687528 yfhG b2555 CDS − 2687693 2689120 yfhK b2556 CDS − 2689678 2693565 purL b2557 CDS + 2693961 2695379 yfhD b2558 CDS − 2695376 2695912 tadA b2559 CDS − 2695937 2696572 yfhB b2560 CDS + 2696781 2697629 yfhH b2561 CDS + 2697685 2697945 yfhL b2562 CDS − 2698640 2699020 acpS b2563 CDS − 2699020 2699751 pdxJ b2564 CDS − 2699763 2700491 recO b2565 CDS − 2700503 2701408 era b2566 CDS − 2701405 2702085 rnc b2567 CDS − 2702357 2703331 lepB b2568 CDS − 2703347 2705146 lepA b2569 CDS − 2705344 2705823 rseC b2570 CDS − 2705820 2706776 rseB b2571 CDS − 2706776 2707426 rseA b2572 CDS − 2707459 2708034 rpoE b2573 CDS + 2708442 2710064 nadB b2574 CDS − 2710049 2710786 yfiC b2575 CDS + 2710918 2712252 srmB b2576 CDS − 2712461 2713342 yfiE b2577 CDS + 2713445 2714032 yfiK b2578 CDS − 2714088 2714471 yfiD b2579 CDS + 2714776 2715465 ung b2580 CDS − 2715513 2716550 yfiF b2581 CDS + 2716757 2717176 trxC b2582 CDS + 2717245 2717943 yfiP b2583 CDS + 2717975 2720635 yfiQ b2584 CDS + 2720749 2722104 pssA b2585 CDS + 2722201 2722473 yfiM b2586 CDS − 2722470 2723765 kgtP b2587 rRNA − 2724091 2724210 rrfG b2588 rRNA − 2724303 2727206 rrlG b2589 tRNA − 2727391 2727466 gltW b2590 rRNA − 2727638 2729179 rrsG b2591 CDS − 2729622 2732195 clpB b2592 CDS − 2732325 2733056 yfiH b2593 CDS − 2733053 2734033 rluD b2594 CDS + 2734168 2734905 yfiO b2595 CDS + 2734935 2735141 b2596 CDS + 2735176 2735517 yfiA b2597 CDS + 2735621 2735668 pheL b2598 CDS + 2735767 2736927 pheA b2599 CDS − 2736970 2738091 tyrA b2600 CDS − 2738102 2739172 aroF b2601 CDS + 2739382 2739747 yfiL b2602 CDS + 2739897 2740415 yfiR b2603 CDS + 2740405 2741631 yfiN b2604 CDS + 2741647 2742129 yfiB b2605 CDS − 2742205 2742552 rplS b2606 CDS − 2742594 2743361 trmD b2607 CDS − 2743392 2743940 rimM b2608 CDS − 2743959 2744207 rpsP b2609 CDS − 2744456 2745817 ffh b2610 CDS + 2745909 2746775 ypjD b2611 CDS − 2748137 2748730 grpE b2614 CDS + 2748853 2749731 yfjB b2615 CDS + 2749817 2751478 recN b2616 CDS + 2751627 2751968 smpA b2617 CDS − 2752030 2752320 yfjF b2618 CDS − 2752310 2752786 yfjG b2619 CDS + 2752918 2753400 smpB b2620 misc_RNA + 2753615 2753977 ssrA b2621 CDS + 2754181 2755422 intA b2622 CDS − 2755666 2756622 yfjH b2623 CDS + 2756666 2756878 alpA b2624 CDS + 2757007 2758416 yfjI b2625 CDS + 2758569 2759195 yfjJ b2626 CDS − 2759373 2761562 yfjK b2627 CDS − 2761559 2763175 yfjL b2628 CDS − 2763535 2763798 yfjM b2629 CDS + 2763940 2765013 yfjN b2630 CDS + 2765006 2765377 yfjO b2631 CDS + 2765732 2766595 yfjP b2632 CDS + 2766687 2767508 yfjQ b2633 CDS + 2767725 2768426 yfjR b2634 CDS + 2768467 2768703 ypjK b2635 CDS + 2768703 2769146 yfjS b2636 CDS + 2769170 2769637 yfjT b2637 CDS − 2769862 2770176 yfjU b2638 CDS − 2770189 2770707 b2639 CDS − 2770858 2771058 b2640 CDS − 2770998 2771180 b2641 CDS + 2771340 2773043 yfjW b2642 CDS + 2773941 2774399 yfjX b2643 CDS + 2774408 2774890 yfjY b2644 CDS + 2775137 2775454 yfjZ b2645 CDS + 2775475 2775804 ypjF b2646 CDS − 2776168 2780748 ypjA b2647 CDS − 2781087 2781230 pinH b2648 CDS − 2781660 2782451 b2649 CDS − 2782551 2783033 b2650 CDS + 2783243 2783374 b2651 tRNA − 2783784 2783859 ileY b2652 CDS − 2783822 2783995 b2653 CDS + 2784419 2784751 b2654 CDS + 2785664 2786260 b2657 CDS + 2786399 2786671 b2658 CDS + 2787007 2787984 b2659 CDS + 2788004 2789272 ygaF b2660 CDS + 2789295 2790743 gabD b2661 CDS + 2790757 2792037 gabT b2662 CDS + 2792275 2793675 gabP b2663 CDS + 2793696 2794358 ygaE b2664 CDS − 2794359 2794808 ygaU b2665 CDS − 2794892 2795050 yqaE b2666 CDS + 2795233 2795532 b2667 CDS + 2795542 2796066 ygaP b2668 CDS − 2796113 2796517 stpA b2669 CDS + 2797186 2797635 b2670 CDS − 2797672 2798016 ygaC b2671 CDS + 2798156 2798497 ygaM b2672 CDS + 2798745 2798990 nrdH b2673 CDS + 2798987 2799397 nrdI b2674 CDS + 2799370 2801514 nrdE b2675 CDS + 2801524 2802483 nrdF b2676 CDS + 2802837 2804039 proV b2677 CDS + 2804032 2805096 proW b2678 CDS + 2805154 2806146 proX b2679 CDS + 2806338 2806604 b2680 CDS + 2806634 2807515 b2681 CDS + 2807639 2808376 ygaZ b2682 CDS + 2808366 2808701 ygaH b2683 CDS + 2808792 2809322 emrR b2684 CDS + 2809449 2810621 emrA b2685 CDS + 2810638 2812176 emrB b2686 CDS − 2812240 2812755 ygaG b2687 CDS − 2812905 2814461 gshA b2688 CDS − 2814534 2814962 yqaA b2689 CDS − 2814959 2815525 yqaB b2690 tRNA − 2815806 2815882 argQ b2691 tRNA − 2816081 2816157 argZ b2692 tRNA − 2816220 2816296 argY b2693 tRNA − 2816495 2816571 argV b2694 tRNA − 2816575 2816667 serV b2695 CDS − 2816983 2817168 csrA b2696 CDS − 2817403 2820033 alaS b2697 CDS − 2820161 2820661 oraA b2698 CDS − 2820730 2821791 recA b2699 CDS − 2821871 2822368 ygaD b2700 CDS − 2822513 2823598 mlt8 b2701 CDS + 2823854 2824417 srlA b2702 CDS + 2824414 2825373 srlE b2703 CDS + 2825384 2825755 srlB b2704 CDS + 2825759 2826538 srlD b2705 CDS + 2826643 2827002 gutM b2706 CDS + 2827069 2827842 srlR b2707 CDS + 2827835 2828800 gutQ b2708 CDS − 2828797 2830311 ygaA b2709 CDS + 2830498 2831937 norV b2710 CDS + 2831934 2833067 ygbD b2711 CDS − 2833195 2835447 hypF b2712 CDS − 2835600 2836127 hydN b2713 CDS − 2836276 2837289 ascG b2714 CDS + 2837546 2839003 ascF b2715 CDS + 2839012 2840436 ascB b2716 CDS − 2840595 2841065 hycI b2717 CDS − 2841058 2841468 hycH b2718 CDS − 2841465 2842232 hycG b2719 CDS − 2842232 2842774 hycF b2720 CDS − 2842784 2844493 hycE b2721 CDS − 2844511 2845434 hycD b2722 CDS − 2845437 2847263 hycC b2723 CDS − 2847260 2847871 hycB b2724 CDS − 2847996 2848457 hycA b2725 CDS + 2848669 2849019 hypA b2726 CDS + 2849023 2849895 hypB b2727 CDS + 2849886 2850158 hypC b2728 CDS + 2850158 2851279 hypD b2729 CDS + 2851318 2852286 hypE b2730 CDS + 2852360 2854438 fhlA b2731 CDS − 2854475 2854828 ygbA b2732 CDS + 2855115 2857676 mutS b2733 CDS + 2857782 2858438 pphB b2734 CDS − 2858489 2859286 ygbI b2735 CDS + 2859452 2860360 ygbJ b2736 CDS + 2860357 2861523 ygbK b2737 CDS + 2861615 2862253 ygbL b2738 CDS + 2862258 2863034 ygbM b2739 CDS + 2863123 2864487 ygbN b2740 CDS − 2864581 2865573 rpoS b2741 CDS − 2865636 2866775 nlpD b2742 CDS − 2866915 2867541 pcm b2743 CDS − 2867535 2868296 surE b2744 CDS − 2868277 2869326 ygbO b2745 CDS − 2869323 2869802 ispF b2746 CDS − 2869802 2870512 ispD b2747 CDS − 2870531 2870842 ygbQ b2748 CDS − 2871036 2871359 ygbE b2749 CDS − 2871409 2872014 cysC b2750 CDS − 2872014 2873441 cysN b2751 CDS − 2873443 2874351 cysD b2752 CDS + 2874603 2875640 iap b2753 CDS − 2876591 2876875 ygbF b2754 CDS − 2876877 2877794 ygbT b2755 CDS − 2877810 2878409 ygcH b2756 CDS − 2878396 2879070 ygcI b2757 CDS − 2879073 2880164 ygcJ b2758 CDS − 2880177 2880659 ygcK b2759 CDS − 2880652 2882160 ygcL b2760 CDS − 2882575 2885241 ygcB b2761 CDS − 2885600 2886334 cysH b2762 CDS − 2886409 2888121 cysI b2763 CDS − 2888121 2889920 cysJ b2764 CDS + 2890236 2890601 ygcM b2765 CDS + 2890679 2891950 ygcN b2766 CDS + 2891941 2892201 ygcO b2767 CDS + 2892218 2892793 ygoP b2768 CDS − 2892941 2893801 ygcQ b2769 CDS − 2893798 2894577 ygcR b2770 CDS − 2894555 2895892 ygcS b2771 CDS − 2897510 2898295 ygcW b2774 CDS + 2898614 2899891 yqcE b2775 CDS + 2899918 2901396 ygcE b2776 CDS − 2902769 2903440 ygcF b2777 CDS + 2903733 2904605 ygcG b2778 CDS − 2904665 2905963 eno b2779 CDS − 2906051 2907688 pyrG b2780 CDS − 2907916 2908707 mazG b2781 CDS − 2908778 2909113 chpA b2782 CDS − 2909113 2909361 chpR b2783 CDS − 2909439 2911673 relA b2784 CDS − 2911721 2913022 rumA b2785 CDS + 2913079 2915835 barA b2786 CDS − 2916067 2917407 gudD b2787 CDS − 2917428 2918768 gudX b2788 CDS − 2918770 2920122 gudP b2789 CDS − 2920557 2921006 yqcA b2790 CDS − 2921024 2921806 yqcB b2791 CDS − 2921806 2922135 yqcC b2792 CDS − 2922757 2923302 syd b2793 CDS + 2923370 2924218 yqcD b2794 CDS + 2924330 2925694 ygdH b2795 CDS + 2926251 2927540 sdaC b2796 CDS + 2927598 2928965 sdaB b2797 CDS + 2928987 2929832 exo b2798 CDS − 2929887 2931038 fucO b2799 CDS − 2931063 2931710 fucA b2800 CDS + 2932257 2933573 fucP b2801 CDS + 2933606 2935381 fucI b2802 CDS + 2935460 2936908 fucK b2803 CDS + 2936910 2937332 fucU b2804 CDS + 2937390 2936121 fucR b2805 CDS − 2938165 2939265 ygdE b2806 CDS − 2939258 2939653 ygdD b2807 CDS − 2939672 2940589 gcvA b2808 CDS − 2940940 2941167 ygdI b2809 CDS + 2941359 2942564 csdA b2810 CDS + 2942564 2943007 ygdK b2811 CDS − 2943058 2943864 ygdL b2812 CDS − 2944103 2945200 mltA b2813 tRNA + 2945409 2945485 metZ b2814 tRNA + 2945519 2945595 metW b2815 tRNA + 2945629 2945705 metV b2816 CDS − 2945779 2947032 amiC b2817 CDS + 2947264 2948595 argA b2818 CDS − 2948657 2950483 recD b2819 CDS − 2950483 2954025 recB b2820 CDS − 2954018 2956906 ptr b2821 CDS − 2957082 2960450 recC b2822 CDS − 2960463 2960786 ppdC b2823 CDS − 2960771 2961178 ygdB b2824 CDS − 2961175 2961738 ppdB b2825 CDS − 2961729 2962199 ppdA b2826 CDS − 2962383 2963177 thyA b2827 CDS − 2963184 2964059 lgt b2828 CDS − 2964210 2966456 ptsP b2829 CDS − 2966469 2966999 ygdP b2830 CDS + 2967684 2968373 mutH b2831 CDS + 2968442 2969155 ygdQ b2832 CDS + 2969293 2969511 ygdR b2833 CDS + 2969619 2970659 tas b2834 CDS − 2970691 2971884 ygeD b2835 CDS − 2971877 2974036 aas b2836 CDS + 2974621 2975652 galR b2837 CDS − 2975659 2976921 lysA b2838 CDS + 2977043 2977978 lysR b2839 CDS − 2977965 2978657 ygeA b2840 CDS − 2978786 2980204 araE b2841 CDS − 2980519 2981280 kduD b2842 CDS − 2981310 2982146 kduI b2843 CDS − 2982433 2983614 yqeF b2844 CDS + 2983869 2985098 yqeG b2845 CDS + 2985558 2986190 yqeH b2846 CDS + 2986524 2987333 yqeI b2847 CDS + 2987326 2987808 yqeJ b2848 CDS − 2987957 2988382 yqeK b2849 CDS + 2988576 2989022 ygeF b2850 CDS + 2989290 2989781 ygeG b2851 CDS + 2990116 2991492 ygeH b2852 CDS + 2991660 2991878 ygeI b2853 CDS + 2992063 2992437 b2854 CDS − 2992482 2992925 ygeK b2855 CDS − 2992959 2993114 b2856 CDS − 2993336 2993767 b2857 CDS − 2993770 2994042 b2858 CDS − 2993984 2994409 b2859 repeat_region + 2994383 2995713 CDS − 2994394 2995299 yi22_4 b2860 CDS − 2995257 2995667 yi21_4 b2861 CDS − 2995711 2996010 ygeP b2862 CDS − 2996056 2996850 ygeQ b2863 tRNA − 2997006 2997079 glyU b2864 CDS − 2997158 2997913 ygeR b2865 CDS + 2998367 3000625 xdhA b2866 CDS + 3000636 3001514 xdhB b2867 CDS + 3001511 3001990 xdhC b2868 CDS − 3002030 3003808 ygeV b2869 CDS + 3004284 3005474 ygeW b2870 CDS + 3005532 3006728 ygeX b2871 CDS + 3006786 3007997 ygeY b2872 CDS + 3008050 3009435 ygeZ b2873 CDS + 3009483 3010415 yqeA b2874 CDS − 3010636 3012261 yqeB b2875 CDS − 3012309 3013079 yqeC b2876 CDS + 3013182 3013760 ygfJ b2877 CDS + 3014082 3017180 ygfK b2878 CDS + 3017183 3018511 ssnA b2879 CDS + 3018562 3019341 ygfM b2880 CDS + 3019338 3022208 xdhD b2881 CDS + 3022373 3023773 ygfO b2882 CDS + 3023788 3025107 guaD b2883 CDS − 3026546 3027034 ygfS b2886 CDS − 3027034 3028953 ygfT b2887 CDS + 3029389 3030837 ygfU b2888 CDS + 3031087 3031635 idi b2889 CDS − 3031679 3033196 lysS b2890 CDS − 3033206 3034304 prfB b2891 CDS − 3034395 3036128 recJ b2892 CDS − 3036134 3036844 dsbC b2893 CDS − 3036869 3037765 xerD b2894 CDS + 3037877 3038398 fldB b2895 CDS − 3038438 3038845 ygfX b2896 CDS − 3038826 3039092 ygfY b2897 CDS + 3039335 3040315 ygfZ b2898 CDS − 3040511 3041170 yqfA b2899 CDS − 3041334 3041645 yqfB b2900 CDS + 3041684 3043123 bglA b2901 CDS − 3043180 3043923 ygfF b2902 CDS − 3044190 3047063 gcvP b2903 CDS − 3047182 3047571 gcvH b2904 CDS − 3047595 3048689 gcvT b2905 CDS − 3049137 3050339 visC b2906 CDS − 3050362 3051540 ubiH b2907 CDS − 3051537 3052862 pepP b2908 CDS − 3052888 3053466 ygfB b2909 CDS + 3053634 3053963 ygfE b2910 misc_RNA + 3054005 3054187 ssrS b2911 CDS + 3054263 3054811 ygfA b2912 CDS − 3055200 3056432 serA b2913 CDS − 3056688 3057347 rpiA b2914 CDS − 3057403 3057633 yqfE b2915 CDS + 3057775 3058668 iciA b2916 CDS + 3058872 3061016 sbm b2917 CDS + 3061009 3062004 argK b2918 CDS + 3062015 3062800 ygfG b2919 CDS + 3062824 3064302 ygfH b2920 CDS − 3064299 3065195 ygfI b2921 CDS − 3065362 3066102 yggE b2922 CDS − 3066195 3066830 yggA b2923 CDS − 3066969 3067829 yggB b2924 CDS − 3068187 3069266 ybaA b2925 CDS − 3069481 3070644 pgk b2926 CDS − 3070694 3071713 epd b2927 CDS − 3071998 3072711 yggC b2928 CDS − 3072708 3073217 yggD b2929 CDS − 3073239 3074204 yggF b2930 CDS − 3075493 3076881 cmtA b2933 CDS − 3076909 3077352 cmtB b2934 CDS − 3077666 3079657 tktA b2935 CDS + 3079935 3080693 yggG b2936 CDS − 3080899 3081819 speB b2937 CDS − 3081957 3083933 speA b2938 CDS − 3083942 3084088 yqgB b2939 CDS + 3084209 3084424 yqgC b2940 CDS − 3084421 3084672 yqgD b2941 CDS + 3084728 3085882 metK b2942 CDS + 3086306 3087700 galP b2943 CDS + 3087777 3088274 sprT b2944 CDS + 3088369 3089076 endA b2945 CDS + 3089156 3089887 yggJ b2946 CDS + 3089900 3090850 gshB b2947 CDS + 3090959 3091522 yqgE b2948 CDS + 3091522 3091938 yqgF b2949 CDS − 3092122 3093102 yggR b2950 CDS + 3093120 3093824 yggS b2951 CDS + 3093842 3094408 yggT b2952 CDS + 3094405 3094695 yggU b2953 CDS + 3094703 3095296 yggV b2954 CDS + 3095289 3096425 yggW b2955 CDS − 3096580 3097587 yggM b2956 CDS − 3097704 3098750 ansB b2957 CDS − 3098926 3099645 yggN b2958 CDS − 3099829 3100155 yggL b2959 CDS − 3100155 3100874 yggH b2960 CDS + 3101035 3102087 mutY b2961 CDS + 3102115 3102390 yggX b2962 CDS + 3102455 3103534 mltC b2963 CDS + 3103736 3104992 nupG b2964 CDS − 3105042 3107177 speC b2965 CDS + 3107575 3108282 yqgA b2966 tRNA + 3108388 3108463 pheV b2967 CDS − 3108612 3109148 yghD b2968 CDS − 3109150 3110010 yghE b2969 CDS − 3110076 3110942 yghF b2970 CDS − 3111089 3111499 yghG b2971 CDS − 3111565 3112374 pppA b2972 CDS − 3117619 3119301 yghK b2975 CDS − 3119656 3121827 glcB b2976 CDS − 3121849 3122253 glcG b2977 CDS − 3124544 3126043 glcD b2979 CDS + 3126294 3127058 glcC b2980 CDS − 3127065 3128237 yghO b2981 repeat_region − 3128168 3129362 CDS + 3128200 3129216 trs5_9 b2982 CDS − 3129363 3130430 yghQ b2983 CDS − 3130476 3131234 yghR b2984 CDS − 3131266 3131979 yghS b2985 CDS + 3132153 3132845 yghT b2986 CDS − 3132894 3134393 pitB b2987 CDS − 3134685 3136544 gss b2988 CDS + 3136749 3137615 yghU b2989 CDS − 3137738 3137986 hybG b2990 CDS − 3137999 3138340 hybF b2991 CDS − 3138333 3138821 hybE b2992 CDS − 3138814 3139308 hybD b2993 CDS − 3139308 3141011 hybC b2994 CDS − 3141008 3142186 hybB b2995 CDS − 3142176 3143162 hybA b2996 CDS − 3143165 3144283 hybO b2997 CDS − 3144472 3144759 yghW b2998 CDS − 3144878 3145288 b2999 CDS − 3145288 3145641 b3000 CDS + 3145919 3146959 yghZ b3001 CDS − 3146999 3147493 yqhA b3002 CDS + 3147684 3148568 yghA b3003 CDS + 3148744 3148989 b3004 CDS − 3148840 3149265 exbD b3005 CDS − 3149272 3150006 exbB b3006 CDS − 3149999 3150154 b3007 CDS + 3150258 3151445 metC b3008 CDS + 3151585 3152244 yghB b3009 CDS − 3152284 3153240 yqhC b3010 CDS + 3153377 3154540 yqhD b3011 CDS + 3154645 3155472 dkgA b3012 CDS + 3155672 3156598 yqhG b3013 CDS + 3156649 3156906 yqhH b3014 CDS − 3159279 3160691 sufI b3017 CDS − 3160766 3161503 plsC b3018 CDS − 3161737 3163995 parC b3019 CDS − 3164133 3165740 ygiS b3020 CDS − 3165873 3166268 ygiT b3021 CDS − 3166270 3166566 ygiU b3022 CDS − 3166771 3167253 b3023 CDS − 3167306 3167696 ygiW b3024 CDS + 3167850 3168509 ygiX b3025 CDS + 3168506 3169855 ygiY b3026 CDS − 3169901 3170233 ygiZ b3027 CDS + 3170552 3171133 mdaB b3028 CDS + 3171164 3171478 ygiN b3029 CDS − 3171526 3173418 parE b3030 CDS − 3173447 3174028 yqiA b3031 CDS − 3174028 3174855 cpdA b3032 CDS − 3174880 3175302 yqiB b3033 CDS − 3175303 3175932 yqiE b3034 CDS + 3176137 3177618 tolC b3035 CDS + 3177618 3177878 ygiA b3036 CDS + 3177862 3178437 ygiB b3037 CDS + 3178443 3179603 ygiC b3038 CDS − 3179641 3180456 YgiD b3039 CDS + 3180572 3181345 ygiE b3040 CDS − 3181835 3182488 ribB b3041 CDS + 3182862 3183152 yqiC b3042 CDS + 3183436 3183987 ygiL b3043 repeat_region − 3184118 3185448 CDS + 3184164 3184574 yi21_5 b3044 CDS + 3184532 3185437 yi22_5 b3045 CDS + 3185422 3187887 yqiG b3046 CDS + 3187903 3188652 yqiH b3047 CDS + 3188654 3189718 yqiI b3048 CDS − 3189761 3189961 glgS b3049 CDS + 3190230 3190859 yqiJ b3050 CDS + 3190886 3192547 yqiK b3051 CDS − 3193342 3194775 rfaE b3052 CDS − 3194823 3197663 glnE b3053 CDS − 3197686 3198987 ygiF b3054 CDS + 3199229 3199849 ygiM b3055 CDS + 3199913 3201151 cca b3056 CDS − 3201332 3202153 bacA b3057 CDS − 3202243 3202611 ygiG b3058 CDS + 3202716 3203333 ygiH b3059 CDS − 3203346 3204278 ygiP b3060 CDS + 3204485 3205396 ttdA b3061 CDS + 3205393 3205998 ttdB b3062 CDS + 3206046 3207509 ygjE b3063 CDS − 3207552 3208565 ygjD b3064 CDS + 3208803 3209018 rpsU b3065 CDS + 3209129 3210874 dnaG b3066 CDS + 3211069 3212910 rpoD b3067 CDS − 3212989 3213495 ygjF b3068 tRNA + 3213620 3213695 ileX b3069 CDS − 3213749 3214513 yqjH b3070 CDS + 3214801 3215424 yqjI b3071 CDS − 3215578 3217098 aer b3072 CDS + 3217489 3218895 ygjG b3073 CDS − 3218937 3219269 ygjH b3074 CDS + 3219488 3220471 ebgR b3075 CDS + 3220655 3223747 ebgA b3076 CDS + 3223744 3224193 ebgC b3077 CDS + 3224256 3225689 ygjI b3078 CDS + 3225823 3226893 ygjJ b3079 CDS + 3226910 3229261 ygjK b3080 CDS + 3229687 3231705 fadH b3081 CDS − 3231750 3232166 ygjM b3082 CDS − 3232163 3232477 ygjN b3083 CDS − 3232761 3233897 ygjO b3084 CDS + 3233982 3234485 ygjP b3085 CDS + 3234562 3235254 ygjQ b3086 CDS + 3235315 3236319 ygjR b3087 CDS + 3236602 3237567 ygjT b3088 CDS + 3237966 3239210 ygjU b3089 CDS − 3239215 3239766 ygjV b3090 CDS − 3239849 3241336 uxaA b3091 CDS − 3241351 3242763 uxaC b3092 CDS + 3243126 3244544 exuT b3093 CDS + 3244674 3245450 exuR b3094 CDS + 3245795 3246457 yqjA b3095 CDS + 3246461 3246844 yqjB b3096 CDS + 3246991 3247359 yqjC b3097 CDS + 3247397 3247702 yqjD b3098 CDS + 3247705 3248109 yqjE b3099 CDS + 3248099 3248398 yqjK b3100 CDS + 3248584 3248976 yqjF b3101 CDS + 3249046 3250032 yqjG b3102 CDS + 3250326 3250691 yhaH b3103 CDS + 3250933 3251289 yhaI b3104 CDS − 3251340 3252236 yhaJ b3105 CDS + 3252341 3253042 yhaK b3106 CDS + 3253065 3253229 yhaL b3107 CDS − 3254701 3255969 yhaO b3110 CDS − 3257743 3258132 tdcF b3113 CDS − 3258146 3260440 tdcE b3114 CDS − 3260474 3261694 tdcD b3115 CDS − 3261708 3263039 tdcC b3116 CDS − 3263061 3264050 tdcB b3117 CDS − 3264149 3265087 tdcA b3118 CDS + 3265402 3265620 tdcR b3119 CDS + 3265876 3266415 yhaB b3120 CDS + 3266437 3267624 yhaC b3121 misc_RNA − 3268238 3268614 rnpB b3123 CDS − 3268647 3269873 garK b3124 CDS − 3269889 3270779 garR b3125 CDS − 3270809 3271579 garL b3126 CDS − 3271595 3272929 garP b3127 CDS + 3273304 3274875 garD b3128 CDS + 3275024 3275359 sohA b3129 CDS + 3275359 3275823 yhaV b3130 CDS − 3275878 3276687 agaR b3131 CDS + 3276936 3278216 agaZ b3132 CDS + 3278239 3278712 agaV b3133 CDS + 3278723 3279124 agaW b3134 CDS + 3279144 3279647 agaA b3135 CDS + 3279998 3281152 agaS b3136 CDS + 3281165 3282025 agaY b3137 CDS + 3282192 3282668 agaB b3138 CDS + 3282707 3283510 agaC b3139 CDS + 3283500 3284291 agaD b3140 CDS + 3284292 3285047 agaI b3141 CDS + 3285448 3286032 yraH b3142 CDS + 3286112 3286807 yraI b3143 CDS + 3286836 3289352 yraJ b3144 CDS + 3289363 3290454 yraK b3145 CDS − 3290497 3291357 yraL b3146 CDS + 3291422 3293458 yraM b3147 CDS + 3293416 3293811 yraN b3148 CDS + 3293831 3294421 yraO b3149 CDS + 3294431 3295006 yraP b3150 CDS − 3295120 3296160 yraQ b3151 CDS − 3296233 3296868 yraR b3152 CDS + 3296996 3297514 yhbO b3153 CDS − 3297494 3297937 yhbP b3154 CDS + 3297988 3298290 yhbQ b3155 CDS − 3298277 3298780 yhbS b3156 CDS − 3298774 3299298 yhbT b3157 CDS + 3299507 3300502 yhbU b3158 CDS + 3300511 3301389 yhbV b3159 CDS + 3301470 3302477 yhbW b3160 CDS − 3302595 3303839 mtr b3161 CDS − 3303993 3305882 deaD b3162 CDS − 3306062 3306946 nlpI b3163 CDS − 3307055 3309259 pnp b3164 CDS − 3309437 3309706 rpsO b3165 CDS − 3309855 3310799 truB b3166 CDS − 3310799 3311200 rbfA b3167 CDS − 3311364 3314036 infB b3168 CDS − 3314061 3315548 nusA b3169 CDS − 3315576 3316028 yhbC b3170 tRNA − 3316235 3316311 metY b3171 CDS + 3316659 3318002 argG b3172 CDS − 3318010 3319635 yhbX b3173 tRNA − 3320094 3320180 leuU b3174 CDS − 3320195 3320527 secG b3175 CDS − 3320755 3322092 glmM b3176 CDS − 3322085 3322933 folP b3177 CDS − 3323023 3324957 hflB b3178 CDS − 3325057 3325686 rrmJ b3179 CDS + 3325812 3326105 yhbY b3180 CDS − 3326261 3326737 greA b3181 CDS + 3326985 3328418 dacB b3182 CDS − 3328604 3329776 yhbZ b3183 CDS − 3329792 3330757 yhbE b3184 CDS − 3330884 3331141 rpmA b3185 CDS − 3331162 3331473 rplU b3186 CDS + 3331732 3332703 ispB b3187 CDS + 3332931 3333209 nlp b3188 CDS − 3333257 3334516 murA b3189 CDS − 3334571 3334825 yrbA b3190 CDS − 3334985 3335278 yrbB b3191 CDS − 3335278 3335913 yrbC b3192 CDS − 3335932 3336483 yrbD b3193 CDS − 3336488 3337270 yrbE b3194 CDS − 3337278 3338087 yrbF b3195 CDS + 3338297 3339274 yrbG b3196 CDS + 3339288 3340274 yrbH b3197 CDS + 3340295 3340861 yrbI b3198 CDS + 3340858 3341433 yrbK b3199 CDS + 3341402 3341959 yhbN b3200 CDS + 3341966 3342691 yhbG b3201 CDS + 3342739 3344172 rpoN b3202 CDS + 3344195 3344482 yhbH b3203 CDS + 3344600 3345091 ptsN b3204 CDS + 3345137 3345991 yhbJ b3205 CDS + 3345988 3346260 ptsO b3206 CDS + 3346474 3347106 yrbL b3207 CDS − 3347103 3347831 mtgA b3208 CDS − 3347828 3348481 yhbL b3209 CDS − 3348711 3351047 arcB b3210 CDS − 3351143 3352072 yhcC b3211 CDS + 3352654 3357207 gltB b3212 CDS + 3357220 3358638 gltD b3213 CDS + 3359198 3359962 gltF b3214 CDS + 3360134 3360808 yhcA b3215 CDS + 3360829 3363210 yhcD b3216 CDS + 3363207 3363686 yhcE b3217 repeat_region + 3363578 3364772 CDS − 3363724 3364740 trs5_10 b3218 CDS + 3364948 3365664 yhcF b3219 CDS + 3365849 3366976 yhcG b3220 CDS − 3367036 3367500 yhcH b3221 CDS − 3367497 3368372 nanK b3222 CDS − 3368369 3369058 nanE b3223 CDS − 3369106 3370596 nanT b3224 CDS − 3370705 3371598 nanA b3225 CDS − 3371720 3372511 nanR b3226 CDS + 3372891 3374258 dcuD b3227 CDS − 3374301 3374798 sspB b3228 CDS − 3374804 3375442 sspA b3229 CDS − 3375837 3376229 rpsI b3230 CDS − 3376245 3376673 rplM b3231 CDS − 3376892 3378019 yhcM b3232 CDS + 3378213 3378611 yhcB b3233 CDS + 3378765 3380132 degQ b3234 CDS + 3380222 3381289 degS b3235 CDS − 3381352 3382290 mdh b3236 CDS + 3382725 3383195 argR b3237 CDS + 3383560 3383823 yhcN b3238 CDS − 3383879 3384151 yhcO b3239 CDS − 3384243 3386210 yhcP b3240 CDS − 3386216 3387148 yhcQ b3241 CDS − 3387156 3387359 yhcR b3242 CDS + 3387542 3388471 yhcS b3243 CDS − 3388605 3390050 tldD b3244 CDS − 3394348 3395817 rng b3247 CDS − 3395807 3396400 yhdE b3248 CDS − 3396409 3396897 mreD b3249 CDS − 3396897 3398000 mreC b3250 CDS − 3398066 3399109 mreB b3251 CDS − 3399414 3401354 yhdA b3252 CDS + 3401506 3402480 yhdH b3253 CDS − 3402538 3402639 b3254 CDS + 3403458 3403928 accB b3255 CDS + 3403939 3405288 accC b3256 CDS + 3405397 3405639 yhdT b3257 CDS + 3405629 3407080 panF b3258 CDS + 3407092 3407973 prmA b3259 CDS + 3408302 3409267 yhdG b3260 CDS + 3409293 3409589 fis b3261 CDS + 3409675 3410559 yhdJ b3262 CDS + 3410643 3410822 yhdU b3263 CDS − 3410825 3411487 envR b3264 CDS + 3411886 3413043 acrE b3265 CDS + 3413055 3416159 acrF b3266 CDS + 3416412 3416633 yhdV b3267 CDS + 3417171 3418088 yhdW b3268 CDS + 3418156 3419337 yhdX b3269 CDS + 3419347 3420450 yhdY b3270 CDS + 3420458 3421216 yhdZ b3271 rRNA − 3421445 3421564 rrfF b3272 tRNA − 3421602 3421677 thrV b3273 rRNA − 3421690 3421809 rrfD b3274 rRNA − 3421902 3424805 rrlD b3275 tRNA − 3424980 3425055 alaU b3276 tRNA − 3425098 3425174 ileU b3277 rRNA − 3425243 3426784 rrsD b3278 CDS + 3427258 3427812 yrdA b3279 CDS − 3427788 3428045 yrdB b3280 CDS − 3428042 3428860 aroE b3281 CDS − 3428865 3429437 yrdC b3282 CDS − 3429442 3429984 yrdD b3283 CDS − 3430013 3430486 smg b3284 CDS + 3431712 3432221 def b3287 CDS + 3432236 3433183 fmt b3288 CDS + 3433229 3434518 rrmB b3289 CDS + 3434540 3435916 trkA b3290 CDS + 3436046 3436456 mscL b3291 CDS − 3436727 3437152 zntR b3292 CDS − 3437163 3437531 yhdN b3293 CDS − 3437638 3438021 rplQ b3294 CDS − 3438062 3439051 rpoA b3295 CDS − 3439077 3439697 rpsD b3296 CDS − 3439731 3440120 rpsK b3297 CDS − 3440137 3440493 rpsM b3298 CDS − 3440640 3440756 rpmJ b3299 CDS − 3440788 3442119 prlA b3300 CDS − 3442127 3442561 rplO b3301 CDS − 3442565 3442744 rpmD b3302 CDS − 3442748 3443251 rpsE b3303 CDS − 3443266 3443619 rplR b3304 CDS − 3443629 3444162 rplF b3305 CDS − 3444175 3444567 rpsH b3306 CDS − 3444601 3444906 rpsN b3307 CDS − 3444921 3445460 rplE b3308 CDS − 3445475 3445789 rplX b3309 CDS − 3445800 3446171 rplN b3310 CDS − 3446336 3446590 rpsQ b3311 CDS − 3446590 3446781 rpmC b3312 CDS − 3446781 3447191 rplP b3313 CDS − 3447204 3447905 rpsC b3314 CDS − 3447923 3448255 rplV b3315 CDS − 3448270 3448548 rpsS b3316 CDS − 3448565 3449386 rplB b3317 CDS − 3449404 3449706 rplW b3318 CDS − 3449703 3450308 rplD b3319 CDS − 3450319 3450948 rplC b3320 CDS − 3450981 3451292 rpsJ b3321 CDS − 3451530 3451949 pioO b3322 CDS − 3451951 3453420 gspA b3323 CDS + 3453600 3454415 gspC b3324 CDS + 3454399 3456351 gspD b3325 CDS + 3456361 3457842 gspE b3326 CDS + 3457839 3459035 gspF b3327 CDS + 3459045 3459482 gspG b3328 CDS + 3459490 3459999 gspH b3329 CDS + 3459996 3460373 gspI b3330 CDS + 3460366 3460953 gspJ b3331 CDS + 3460946 3461929 gspK b3332 CDS + 3461944 3463107 gspL b3333 CDS + 3463104 3463565 gspM b3334 CDS + 3463565 3464242 gspO b3335 CDS − 3464271 3464747 bfr b3336 CDS − 3464819 3465013 bfd b3337 CDS − 3465182 3467875 chiA b3338 CDS − 3468167 3469351 tufA b3339 CDS − 3469422 3471536 fusA b3340 CDS − 3471564 3472103 rpsG b3341 CDS − 3472200 3472574 rpsL b3342 CDS − 3472700 3472987 yheL b3343 CDS − 3472995 3473354 yheM b3344 CDS − 3473354 3473740 yheN b3345 CDS − 3473740 3474462 yheO b3346 CDS − 3474629 3475441 fkpA b3347 CDS + 3475662 3475880 slyX b3348 CDS − 3475929 3476519 slyD b3349 CDS − 3476824 3478629 kefB b3350 CDS − 3478629 3479183 yheR b3351 CDS + 3479311 3481224 yheS b3352 CDS + 3481224 3482246 yheT b3353 CDS + 3482240 3482458 yheU b3354 CDS + 3482512 3483381 prkB b3355 CDS − 3483436 3483840 yhfA b3356 CDS + 3484142 3484774 crp b3357 CDS + 3484813 3486915 yhfK b3358 CDS − 3486982 3488202 argD b3359 CDS − 3488288 3488851 pabA b3360 CDS − 3488883 3489485 fic b3361 CDS − 3489475 3489642 yhfG b3362 CDS − 3489747 3490319 pplA b3363 CDS + 3490590 3491771 yhfC b3364 CDS + 3492033 3494576 nirB b3365 CDS + 3494573 3494899 nirD b3366 CDS + 3495025 3495831 nirC b3367 CDS + 3495850 3497223 cysG b3368 CDS + 3497470 3497637 yhfL b3369 CDS + 3497932 3499269 yhfM b3370 CDS + 3499290 3500312 yhfN b3371 CDS + 3501189 3501974 yhfQ b3374 CDS + 3502074 3502805 yhfR b3375 CDS − 3502957 3504042 yhfS b3376 CDS − 3504054 3505358 yhfT b3377 CDS − 3505370 3505723 yhfU b3378 CDS − 3505734 3506612 yhfV b3379 CDS − 3506609 3507835 yhfW b3380 CDS − 3507835 3508998 yhfX b3381 CDS − 3509082 3509444 yhfY b3382 CDS − 3509461 3510366 yhfZ b3383 CDS − 3510656 3511660 trpS b3384 CDS − 3511653 3512411 gph b3385 CDS − 3512404 3513081 rpe b3386 CDS − 3513099 3513935 dam b3387 CDS − 3514042 3515328 damX b3388 CDS − 3515420 3516508 aroB b3389 CDS − 3516565 3517086 aroK b3390 CDS − 3517487 3518725 hofQ b3391 CDS − 3518637 3519041 yrfA b3392 CDS − 3519031 3519471 yrfB b3393 CDS − 3519455 3519994 yrfC b3394 CDS − 3519994 3520773 yrfD b3395 CDS + 3520893 3523445 mrcA b3396 CDS − 3523611 3524171 yrfE b3397 CDS + 3524491 3526626 yrfF b3398 CDS + 3526691 3527359 yrfG b3399 CDS + 3527370 3527771 hslR b3400 CDS + 3527796 3528674 hslO b3401 CDS − 3528737 3530461 yhgE b3402 CDS + 3530840 3532462 pckA b3403 CDS − 3532538 3533890 envZ b3404 CDS − 3533887 3534606 ompR b3405 CDS + 3534834 3535310 greB b3406 CDS + 3535407 3537728 yhgF b3407 CDS + 3538185 3538412 feoA b3408 CDS + 3538429 3540750 feoB b3409 CDS + 3540750 3540986 yhgG b3410 CDS + 3541189 3542067 yhgA b3411 CDS − 3542096 3542866 bioH b3412 CDS + 3542904 3543587 yhgH b3413 CDS + 3543646 3544221 yhgI b3414 CDS + 3544581 3545897 gntT b3415 CDS − 3546008 3548092 malQ b3416 CDS − 3548102 3550495 malP b3417 CDS + 3551107 3553812 malT b3418 CDS − 3554875 3556101 rtcB b3421 CDS + 3556290 3557888 rtcR b3422 CDS − 3557870 3558628 glpR b3423 CDS − 3558645 3559475 glpG b3424 CDS − 3559520 3559846 glpE b3425 CDS + 3560036 3561541 glpD b3426 CDS − 3561747 3562028 yzgL b3427 CDS − 3562157 3564604 glgP b3428 CDS − 3564623 3566056 glgA b3429 CDS − 3566056 3567351 glgC b3430 CDS − 3567369 3569342 glgX b3431 CDS − 3569339 3571525 glgB b3432 CDS − 3571798 3572901 asd b3433 CDS + 3573094 3573687 yhgN b3434 CDS − 3575088 3575615 gntK b3437 CDS − 3575754 3576749 gntR b3438 CDS − 3576973 3577668 yhhW b3439 CDS − 3577791 3578828 yhhX b3440 CDS + 3579161 3579649 yhhY b3441 CDS + 3579886 3581064 yhhZ b3442 CDS + 3581064 3581477 yrhA b3443 repeat_region + 3581451 3582218 CDS + 3581506 3581781 insA_6 b3444 CDS + 3581700 3582203 insB_6 b3445 CDS + 3582782 3583066 yrhB b3446 CDS − 3583104 3584846 ggt b3447 CDS + 3584966 3585406 yhhA b3448 CDS − 3585393 3586136 ugpQ b3449 CDS − 3586133 3587203 ugpC b3450 CDS − 3587205 3588050 ugpE b3451 CDS − 3588047 3588934 ugpA b3452 CDS − 3589032 3590348 ugpB b3453 CDS − 3590747 3591460 livF b3454 CDS − 3591462 3592229 livG b3455 CDS − 3592226 3593503 livM b3456 CDS − 3593500 3594426 livH b3457 CDS − 3594474 3595583 livK b3458 CDS + 3596007 3596390 yhhK b3459 CDS − 3596578 3597681 livJ b3460 CDS − 3597952 3598806 rpoH b3461 CDS − 3599051 3600109 ftsX b3462 CDS − 3600102 3600770 ftsE b3463 CDS − 3600773 3602266 ftsY b3464 CDS + 3602416 3603012 yhhF b3465 CDS + 3603002 3603271 yhhL b3466 CDS − 3603274 3603633 yhhM b3467 CDS + 3603774 3604400 yhhN b3468 CDS + 3604474 3606672 zntA b3469 CDS − 3606774 3607019 sirA b3470 CDS + 3607240 3607905 yhhQ b3471 CDS + 3607978 3608535 dcrB b3472 CDS − 3608539 3609756 yhhS b3473 CDS + 3609888 3610937 yhhT b3474 CDS + 3610992 3611579 acpT b3475 CDS + 3611690 3613264 nikA b3476 CDS + 3613264 3614208 nikB b3477 CDS + 3614205 3615038 nikC b3478 CDS + 3615038 3615802 nikD b3479 CDS + 3615799 3616605 nikE b3480 CDS + 3616611 3617012 nikR b3481 CDS + 3617215 3621450 rhsB b3482 CDS + 3621422 3621805 yhhH b3483 CDS + 3622401 3623537 yhhI b3484 CDS − 3623702 3624826 yhhJ b3485 CDS − 3624826 3627561 yhiH b3486 CDS − 3627558 3628625 yhiI b3487 CDS − 3628991 3630613 yhiJ b3488 CDS − 3630875 3631267 yhiK b3489 CDS − 3631243 3632481 yhiL b3490 CDS + 3632864 3633916 yhiM b3491 CDS − 3634231 3635433 yhiN b3492 CDS + 3635665 3637164 pitA b3493 CDS − 3637408 3637743 yhiO b3494 CDS + 3638134 3638568 uspA b3495 CDS + 3638885 3640354 yhiP b3496 CDS − 3640403 3641156 yhiQ b3497 CDS − 3641163 3643205 prlC b3498 CDS + 3643408 3644250 yhiR b3499 CDS + 3644322 3645674 gor b3500 CDS + 3646551 3646904 arsR b3501 CDS + 3646958 3648247 arsB b3502 CDS + 3648260 3648685 arsC b3503 CDS + 3649314 3650096 yhiS b3504 repeat_region + 3650059 3651253 CDS − 3650205 3651221 trs5_11 b3505 CDS + 3651984 3652550 slp b3506 CDS + 3652706 3653236 yhiF b3507 CDS − 3653278 3653925 yhiD b3508 CDS − 3653989 3654315 hdeB b3509 CDS − 3654431 3654763 hdeA b3510 CDS + 3655018 3655590 hdeD b3511 CDS + 3656389 3656916 yhiE b3512 CDS + 3657255 3658412 yhiU b3513 CDS + 3658437 3661550 yhiV b3514 CDS − 3661913 3662641 yhiW b3515 CDS − 3663009 3663833 gadX b3516 CDS − 3664203 3665603 gadA b3517 CDS − 3665814 3667211 yhjA b3518 CDS + 3667615 3669264 treF b3519 CDS − 3669315 3669917 yhjB b3520 CDS + 3670365 3671336 yhjC b3521 CDS + 3671385 3672398 yhjD b3522 CDS + 3672809 3674131 yhjE b3523 CDS − 3674313 3676388 yhjG b3524 CDS − 3676443 3677210 yhjH b3525 CDS + 3677442 3678371 kdgK b3526 CDS − 3678467 3679963 yhjJ b3527 CDS − 3680184 3681470 dctA b3528 CDS − 3681653 3683602 yhjk b3529 CDS − 3683723 3687196 yhiL b3530 CDS − 3687178 3688284 bcsC b3531 CDS − 3688291 3690630 yhjN b3532 CDS − 3690641 3693259 yhjO b3533 CDS − 3693256 3693984 yhjQ b3534 CDS − 3694020 3694208 yhjR b3535 CDS + 3694481 3696052 yhiS b3536 CDS + 3696049 3696240 yhiT b3537 CDS + 3696237 3697916 yhjU b3538 CDS + 3698586 3699857 yhjV b3539 CDS − 3699887 3700891 dppF b3540 CDS − 3700888 3701871 dppD b3541 CDS − 3701882 3702784 dppC b3542 CDS − 3702794 3703813 dppB b3543 CDS − 3704121 3705728 dppA b3544 tRNA − 3706639 3706715 proK b3545 CDS − 3706807 3708498 yhjW b3546 CDS − 3708822 3710030 yhjX b3547 CDS − 3710259 3710957 yhjY b3548 CDS + 3711115 3711678 tag b3549 CDS + 3711675 3712115 yiaC b3550 CDS − 3712084 3714363 bisC b3551 CDS + 3714570 3715229 yiaD b3552 CDS + 3715333 3716307 tkrA b3553 CDS − 3716357 3717067 yiaF b3554 CDS + 3717501 3717791 yiaG b3555 CDS + 3718072 3718284 cspA b3556 repeat_region + 3718656 3720098 CDS + 3718703 3719224 yi5A b3557 CDS + 3719221 3720072 t150 b3558 CDS − 3720351 3722420 glyS b3559 CDS − 3722430 3723341 glyQ b3560 CDS + 3723910 3724905 yiaH b3561 CDS − 3724947 3725384 yiaA b3562 CDS − 3725430 3725771 yiaB b3563 CDS − 3725940 3727394 xylB b3564 CDS − 3727466 3728788 xylA b3565 CDS + 3729154 3730146 xylF b3566 CDS + 3730224 3731765 xylG b3567 CDS + 3731743 3732924 xylH b3568 CDS + 3733002 3734180 xylR b3569 CDS − 3734376 3735200 bax b3570 CDS + 3735520 3737550 malS b3571 CDS + 3737728 3738981 avtA b3572 CDS − 3739132 3739605 yiaI b3573 CDS − 3739707 3740555 yiaJ b3574 CDS + 3740756 3741754 yiaK b3575 CDS + 3741766 3742233 yiaL b3576 CDS + 3742351 3742824 yiaM b3577 CDS + 3742827 3744104 yiaN b3578 CDS + 3744117 3745103 yiaO b3579 CDS + 3745107 3746603 lyxK b3580 CDS + 3746600 3747262 sgbH b3581 CDS + 3747255 3748115 sgbU b3582 CDS + 3748109 3748804 sgbE b3583 CDS − 3749151 3749891 yiaT b3584 CDS + 3750015 3750989 yiaU b3585 CDS − 3750986 3752122 yiaV b3586 CDS − 3752128 3752451 yiaW b3587 CDS − 3752996 3754534 aldB b3588 CDS − 3754699 3755850 yiaY b3589 CDS − 3756040 3757884 selB b3590 CDS − 3757881 3759272 selA b3591 CDS − 3759370 3759978 yibF b3592 CDS + 3760206 3764339 rhsA b3593 CDS + 3764360 3765202 yibA b3594 CDS + 3765244 3765945 yibJ b3595 CDS + 3766200 3766661 yibG b3596 CDS − 3768266 3769402 yibH b3597 CDS − 3769405 3769767 yibI b3598 CDS + 3770304 3772217 mtlA b3599 CDS + 3772447 3773595 mtlD b3600 CDS + 3773595 3774182 mtlR b3601 CDS + 3774688 3775050 yibL b3602 CDS + 3775422 3777077 lldP b3603 CDS + 3777077 3777853 lldR b3604 CDS + 3777850 3779040 lldD b3605 CDS + 3779238 3779711 yibK b3606 CDS − 3779764 3780585 cysE b3607 CDS − 3780665 3781684 gpsA b3608 CDS − 3781684 3782151 secB b3609 CDS − 3782214 3782465 grxC b3610 CDS − 3782607 3783038 yibN b3611 CDS + 3783283 3784827 yibO b3612 CDS + 3784861 3786120 yibP b3613 CDS + 3786124 3787083 yibQ b3614 CDS − 3787070 3788104 yibD b3615 CDS − 3788343 3789368 tdh b3616 CDS − 3789378 3790574 kbl b3617 CDS − 3790849 3791706 htrL b3618 CDS + 3792010 3792942 rfaD b3619 CDS + 3792952 3793998 rfaF b3620 CDS + 3794002 3794961 rfaC b3621 CDS + 3794971 3796230 rfaL b3622 CDS − 3796262 3797335 rfaK b3623 CDS − 3797368 3798219 rfaZ b3624 CDS − 3798290 3798988 rfaY b3625 CDS − 3799006 3800022 rfaJ b3626 CDS − 3800062 3801081 rfaI b3627 CDS − 3801081 3802190 rfaB b3628 CDS − 3802204 3803139 rfaS b3629 CDS − 3803176 3803973 rfaP b3630 CDS − 3803966 3805090 rfaG b3631 CDS − 3805087 3806121 rfaQ b3632 CDS + 3806563 3807840 kdtA b3633 CDS + 3807848 3808327 coaD b3634 CDS − 3808366 3809175 mutM b3635 CDS − 3809273 3809440 rpmG b3636 CDS − 3809461 3809697 rpmB b3637 CDS − 3809914 3810582 radC b3638 CDS + 3810754 3811974 dfp b3639 CDS + 3811955 3812410 dut b3640 CDS + 3812517 3813113 ttk b3641 CDS − 3813150 3813791 pyrE b3642 CDS − 3813886 3814572 rph b3643 CDS + 3814699 3815562 yicC b3644 CDS + 3815783 3816607 dinD b3645 CDS + 3816897 3817514 yicG b3646 CDS − 3817511 3819193 yicF b3647 CDS + 3819451 3820074 gmk b3648 CDS + 3820129 3820404 rpoZ b3649 CDS + 3820423 3822531 spoT b3650 CDS + 3822538 3823227 trmH b3651 CDS + 3823233 3825314 recG b3652 CDS − 3825483 3826688 gltS b3653 CDS + 3826968 3828359 yicE b3654 CDS + 3828480 3830189 yicH b3655 CDS − 3830242 3832560 yicI b3656 CDS − 3832570 3833952 yicJ b3657 tRNA + 3834245 3834339 selC b3658 CDS + 3834976 3836160 yicK b3659 CDS + 3836271 3837194 yicL b3660 CDS − 3837198 3838016 nlpA b3661 CDS − 3838572 3839927 yicM b3662 CDS − 3839973 3840425 yicN b3663 CDS − 3840478 3841812 yicO b3664 CDS + 3841987 3843753 ade b3665 CDS − 3843799 3845190 uhpT b3666 CDS − 3845328 3846647 uhpC b3667 CDS − 3846657 3848159 uhpB b3668 CDS − 3848159 3848749 uhpA b3669 CDS − 3848825 3849115 ilvN b3670 CDS − 3849119 3850807 ilvB b3671 CDS − 3850913 3851011 ivbL b3672 CDS + 3851945 3853129 emrD b3673 CDS − 3853137 3853634 yidE b3674 CDS − 3853631 3853993 yidG b3675 CDS − 3853983 3854330 yidH b3676 CDS + 3854438 3854887 yidI b3677 CDS − 3854934 3856427 yidJ b3678 CDS − 3856424 3858139 yidK b3679 CDS + 3858276 3859199 yidL b3680 CDS − 3859372 3860010 glvG b3681 CDS − 3860010 3860495 glvB b3682 CDS − 3860520 3861626 glvC b3683 CDS + 3861922 3862638 yidP b3684 CDS − 3862635 3864296 yidE b3685 CDS − 3864492 3864920 ibpB b3686 CDS − 3865032 3865445 ibpA b3687 CDS + 3865751 3866083 yidQ b3688 CDS − 3866085 3867299 yidR b3689 CDS + 3867400 3868464 yidS b3690 CDS − 3868461 3869753 dgoT b3691 CDS − 3871619 3872497 dgoK b3693 CDS + 3873461 3874117 yidX b3696 CDS − 3874163 3874975 yidA b3697 CDS − 3875090 3875488 yidB b3698 CDS − 3875728 3878142 gyrB b3699 CDS − 3878171 3879244 recF b3700 CDS − 3879244 3880344 dnaN b3701 CDS − 3880349 3881752 dnaA b3702 CDS + 3882359 3882499 rpmH b3703 CDS + 3882516 3882875 rnpA b3704 CDS + 3883099 3884745 yidC b3705 CDS + 3884851 3886215 trmE b3706 CDS + 3886458 3886532 tnaL b3707 CDS + 3886753 3888168 tnaA b3708 CDS + 3888259 3889506 tnaB b3709 CDS + 3889638 3890813 yidY b3710 CDS + 3890788 3891747 yidZ b3711 CDS + 3891892 3892653 yieE b3712 CDS + 3892675 3893241 yieF b3713 CDS − 3893295 3894632 yieG b3714 CDS + 3894797 3895462 yieH b3715 CDS + 3895529 3895996 yieI b3716 CDS + 3896045 3896632 yieJ b3717 CDS − 3896694 3897416 yieK b3718 CDS − 3897431 3898627 yieL b3719 CDS − 3898627 3900243 yieC b3720 CDS − 3900312 3901724 bglB b3721 CDS − 3901743 3903620 bglF b3722 CDS − 3903754 3904590 bglG b3723 CDS − 3904876 3905601 phoU b3724 CDS − 3905616 3906389 pstB b3725 CDS − 3906572 3907462 pstA b3726 CDS − 3907462 3908421 pstC b3727 CDS − 3908508 3909548 pstS b3728 CDS − 3909862 3911691 glmS b3729 CDS − 3911853 3913223 glmU b3730 CDS − 3913576 3913995 atpC b3731 CDS − 3914016 3915398 atpD b3732 CDS − 3915425 3916288 atpG b3733 CDS − 3916339 3917880 atpA b3734 CDS − 3917893 3918426 atpH b3735 CDS − 3918441 3918911 atpF b3736 CDS − 3918973 3919212 atpE b3737 CDS − 3919259 3920074 atpB b3738 CDS − 3920083 3920463 atpI b3739 CDS − 3921080 3921703 gidB b3740 CDS − 3921767 3923656 gidA b3741 rep_origin + 3923767 3923998 b4489 CDS − 3924035 3924478 mioC b3742 CDS − 3924568 3925026 asnC b3743 CDS + 3925178 3926170 asnA b3744 CDS − 3926175 3927626 yieM b3745 CDS − 3927620 3929116 yieN b3746 CDS + 3929339 3931207 kup b3747 CDS + 3931374 3931793 rbsD b3748 CDS + 3931801 3933306 rbsA b3749 CDS + 3933311 3934276 rbsC b3750 CDS + 3934301 3935191 rbsB b3751 CDS + 3935317 3936246 rbsK b3752 CDS + 3936250 3937242 rbsR b3753 CDS − 3937208 3938635 yieO b3754 CDS − 3938658 3939350 yieP b3755 rRNA + 3939831 3941372 rrsC b3756 tRNA + 3941458 3941533 gltU b3757 rRNA + 3941727 3944630 rrlC b3758 rRNA + 3944723 3944842 rrfC b3759 tRNA + 3944895 3944971 aspT b3760 tRNA + 3944980 3945055 trpT b3761 CDS + 3946109 3946447 yifE b3764 CDS − 3946472 3948022 yifB b3765 CDS + 3948345 3948443 ilvL b3766 CDS + 3948583 3949566 ilvG_1 b3767 CDS + 3949646 3950227 ilvG_2 b3768 CDS + 3950224 3950487 ilvM b3769 CDS + 3950507 3951436 ilvE b3770 CDS + 3951501 3953351 ilvD b3771 CDS + 3953354 3954898 ilvA b3772 CDS − 3954950 3955843 ilvY b3773 CDS + 3955993 3957468 ilvC b3774 CDS − 3957555 3957836 ppiC b3775 CDS − 3958035 3958310 b3776 CDS − 3958265 3958483 yifN b3777 CDS + 3958700 3960721 rep b3778 CDS − 3960768 3962252 gppA b3779 CDS − 3962388 3963653 rhlB b3780 CDS + 3963784 3964113 trxA b3781 CDS + 3964254 3964355 rhoL b3782 CDS + 3964440 3965699 rho b3783 CDS + 3965939 3967042 rfe b3784 CDS + 3967054 3968100 wzzE b3785 CDS + 3968156 3969286 wecB b3786 CDS + 3969283 3970545 wecC b3787 CDS + 3970545 3971612 rffG b3788 CDS + 3971631 3972512 rffH b3789 CDS + 3972619 3973164 wecD b3790 CDS + 3973169 3974299 wecE b3791 CDS + 3974301 3975551 wzxE b3792 CDS + 3976624 3977976 wzyE b3793 CDS + 3977979 3978719 wecG b3794 CDS + 3978910 3980295 yifK b3795 tRNA + 3980398 3980474 argX b3796 tRNA + 3980532 3980608 hisR b3797 tRNA + 3980629 3980715 leuT b3798 tRNA + 3980758 3980834 proM b3799 CDS + 3980981 3982216 aslB b3800 CDS − 3982375 3984030 aslA b3801 CDS − 3984709 3985905 hemY b3802 CDS − 3985908 3987089 hemX b3803 CDS − 3987111 3987851 hemD b3804 CDS − 3987848 3988789 hemC b3805 CDS + 3989176 3991722 cyaA b3806 CDS − 3991762 3992082 cyaY b3807 CDS + 3991873 3992358 yzcX b3808 CDS + 3992785 3993609 dapF b3809 CDS + 3993606 3994313 yigA b3810 CDS + 3994310 3995206 xerC b3811 CDS + 3995206 3995922 yigB b3812 CDS + 3996006 3998168 uvrD b3813 CDS + 3999449 4000399 corA b3816 CDS − 4000442 4000822 yigF b3817 CDS − 4000836 4001216 yigG b3818 CDS − 4001311 4002201 rarD b3819 CDS − 4002253 4002720 yigI b3820 CDS + 4002885 4003754 pldA b3821 CDS + 4003887 4005716 recQ b3822 CDS + 4005780 4006400 rhtC b3823 CDS − 4006462 4007082 rhtB b3824 CDS + 4007193 4008215 pldB b3825 CDS + 4008223 4009023 yigL b3826 CDS + 4009099 4009998 yigM b3827 CDS − 4009886 4010839 metR b3828 CDS + 4011076 4013337 metE b3829 CDS − 4013377 4014192 ysgA b3830 CDS + 4014454 4015215 udp b3831 CDS + 4015356 4016783 yigN b3832 CDS + 4016878 4017633 ubiE b3833 CDS + 4017647 4018252 yigP b3834 CDS + 4018249 4019889 ubiB b3835 CDS + 4019968 4020237 tatA b3836 CDS + 4020241 4020756 tatB b3838 CDS + 4020759 4021535 tatC b3839 CDS − 4022356 4022844 rfaH b3842 CDS + 4023011 4024504 ubiD b3843 CDS + 4024550 4025251 fre b3844 CDS − 4025632 4026795 fadA b3845 CDS − 4026805 4028994 fadB b3846 CDS + 4029184 4030515 pepQ b3847 CDS + 4030515 4031129 yigZ b3848 CDS + 4031168 4032619 trkH b3849 CDS + 4032631 4033176 hemG b3850 rRNA + 4033554 4035095 rrsA b3851 tRNA + 4035164 4035240 ileT b3852 tRNA + 4035283 4035358 alaT b3853 rRNA + 4035542 4038446 rrlA b3854 rRNA + 4038540 4038659 rrfA b3855 CDS − 4038929 4039441 mobB b3856 CDS − 4039438 4040022 mobA b3857 CDS + 4040092 4040361 yihD b3858 CDS + 4040438 4041424 yihE b3859 CDS + 4041441 4042067 dsbA b3860 CDS + 4042222 4043652 yihF b3861 CDS − 4043693 4044625 yihG b3862 CDS + 4044989 4047775 polA b3863 misc_RNA + 4047922 4048030 spf b3864 CDS − 4048156 4048752 yihA b3865 CDS + 4049370 4049879 yihI b3866 CDS + 4050068 4051441 hemN b3867 CDS − 4051892 4053301 glnG b3868 CDS − 4053313 4054362 glnL b3869 CDS − 4054648 4056057 glnA b3870 CDS + 4056430 4058253 bipA b3871 CDS + 4058470 4059180 yihL b3872 CDS + 4059188 4060168 yihM b3873 CDS + 4060270 4061535 yihN b3874 CDS − 4061626 4062318 yshA b3875 CDS − 4062386 4063789 yihO b3876 CDS − 4063832 4065238 yihP b3877 CDS − 4065263 4067299 yihQ b3878 CDS − 4067498 4068424 yihR b3879 CDS − 4068538 4069779 yihS b3880 CDS − 4069796 4070674 yihT b3881 CDS − 4070698 4071594 yihU b3882 CDS + 4071762 4072658 yihV b3883 CDS + 4072692 4073477 yihW b3884 CDS + 4073576 4074175 yihX b3885 CDS + 4074169 4075041 rbn b3886 CDS + 4075038 4075475 dtd b3887 CDS + 4075472 4076461 yiiD b3888 CDS + 4077314 4077532 yiiE b3889 CDS + 4077774 4077992 yiiF b3890 CDS − 4078322 4079251 fdhE b3891 CDS − 4079248 4079883 fdoI b3892 CDS − 4079880 4080782 fdoH b3893 CDS − 4080795 4083845 fdoG b3894 CDS + 4084039 4084872 fdhD b3895 CDS + 4085025 4086080 yiiG b3896 CDS − 4086130 4087878 frvR b3897 CDS − 4087878 4088948 frvX b3898 CDS − 4088938 4090389 frvB b3899 CDS − 4090400 4090846 frvA b3900 CDS − 4091147 4091461 yiiL b3901 CDS − 4091471 4092295 rhaD b3902 CDS − 4092746 4094005 rhaA b3903 CDS − 4094002 4095471 rhaB b3904 CDS + 4095759 4096595 rhaS b3905 CDS + 4096579 4097517 rhaR b3906 CDS − 4097514 4098548 rhaT b3907 CDS + 4098833 4099453 sodA b3908 CDS + 4099713 4100696 kdgT b3909 CDS + 4100845 4101519 yiiM b3910 CDS − 4101625 4102998 cpxA b3911 CDS − 4102995 4103693 cpxR b3912 CDS + 4104492 4105394 yiiP b3915 CDS + 4105575 4106537 pfkA b3916 CDS + 4106857 4107846 sbp b3917 CDS + 4107953 4108708 cdh b3918 CDS − 4108763 4109530 tpiA b3919 CDS − 4109638 4110237 yiiQ b3920 CDS + 4110338 4110778 yiiR b3921 CDS + 4110990 4111289 yiiS b3922 CDS + 4111316 4111744 yiiT b3923 CDS − 4111749 4112495 fpr b3924 CDS − 4112592 4113602 glpX b3925 CDS − 4113737 4115245 glpK b3926 CDS − 4115268 4116113 glpE b3927 CDS + 4116538 4116783 yiiU b3928 CDS − 4116868 4117353 menG b3929 CDS − 4117446 4118372 menA b3930 CDS − 4118439 4119770 hslU b3931 CDS − 4119780 4120310 hslV b3932 CDS − 4120403 4121362 ftsN b3933 CDS − 4121454 4122479 cytR b3934 CDS − 4122635 4124833 priA b3935 CDS + 4125036 4125248 rpmE b3936 CDS − 4125309 4125917 yiiX b3937 CDS − 4126101 4126418 metJ b3938 CDS + 4126695 4127855 metB b3939 CDS + 4127858 4130290 metL b3940 CDS + 4130639 4131529 metF b3941 CDS + 4131858 4134038 katG b3942 CDS + 4134131 4135036 yijE b3943 CDS − 4135063 4135680 yijF b3944 CDS − 4135955 4137058 gldA b3945 CDS − 4137069 4137731 talC b3946 CDS − 4137743 4140244 ptsA b3947 CDS + 4140553 4141632 frwC b3949 CDS + 4141647 4141967 frwB b3950 CDS + 4142018 4144315 pflD b3951 CDS + 4144281 4145159 pflC b3952 CDS + 4145161 4145502 frwD b3953 CDS − 4145489 4146340 yijO b3954 CDS − 4146555 4148288 yijP b3955 CDS − 4148470 4151121 ppc b3956 CDS − 4151719 4152870 argE b3957 CDS + 4153024 4154028 argC b3958 CDS + 4154039 4154812 argB b3959 CDS + 4154873 4156246 argH b3960 CDS + 4156513 4157430 oxyR b3961 CDS − 4157413 4158813 udhA b3962 CDS + 4159090 4159794 yijC b3963 CDS + 4159794 4160153 yijD b3964 CDS − 4160193 4161293 trmA b3965 CDS + 4161662 4163506 btuB b3966 CDS + 4163451 4164308 murI b3967 rRNA + 4164682 4166223 rrsB b3968 tRNA + 4166395 4166470 gltT b3969 rRNA + 4166664 4169567 rrlB b3970 rRNA + 4169660 4169779 rrfB b3971 CDS + 4170080 4171108 murB b3972 CDS + 4171105 4172070 birA b3973 CDS − 4172099 4173049 coaA b3974 CDS − 4173236 4173391 b3975 tRNA + 4173411 4173486 thrU b3976 tRNA + 4173495 4173579 tyrU b3977 tRNA + 4173698 4173770 glyT b3978 tRNA + 4173777 4173852 thrT b3979 CDS + 4173967 4175151 tufB b3980 CDS + 4175381 4175764 secE b3981 CDS + 4175766 4176311 nusG b3982 CDS + 4176470 4176898 rplK b3983 CDS + 4176902 4177606 rplA b3984 CDS + 4178019 4178516 rplJ b3985 CDS + 4178583 4178948 rplL b3986 CDS + 4179268 4183296 rpoB b3987 CDS + 4183373 4187596 rpoC b3988 CDS + 4187809 4188348 htrC b3989 CDS − 4188758 4189891 thiH b3990 CDS − 4189888 4190658 thiG b3991 CDS − 4190844 4191599 thiF b3992 CDS − 4191592 4192227 thiE b3993 CDS − 4192227 4194122 thiC b3994 CDS − 4194355 4194831 rsd b3995 CDS + 4194926 4195699 nudC b3996 CDS + 4195739 4196803 hemE b3997 CDS + 4196813 4197484 nfi b3998 CDS + 4197527 4198117 yjaG b3999 CDS + 4198304 4198576 hupA b4000 CDS + 4198589 4199284 yjaH b4001 CDS − 4199286 4199711 zraP b4002 CDS + 4199949 4201346 zraS b4003 CDS + 4201343 4202668 zraR b4004 CDS − 4202665 4203954 purD b4005 CDS − 4203966 4205555 purH b4006 rRNA + 4206170 4207711 rrsE b4007 tRNA + 4207797 4207872 gltV b4008 rRNA + 4208066 4210969 rrlE b4009 rRNA + 4211063 4211182 rrfE b4010 CDS + 4211257 4211640 yjaA b4011 CDS − 4211703 4212146 yjaB b4012 CDS + 4212303 4213232 metA b4013 CDS + 4213501 4215102 aceB b4014 CDS + 4215132 4216436 aceA b4015 CDS + 4216619 4218355 aceK b4016 CDS − 4218324 4220510 arpA b4017 CDS − 4220827 4221651 iclR b4018 CDS + 4221851 4225534 metH b4019 CDS + 4225754 4227385 yjbB b4020 CDS − 4227476 4228165 pepE b4021 CDS + 4228377 4229249 yjbC b4022 CDS − 4229382 4229654 yjbD b4023 CDS − 4229907 4231256 lysC b4024 CDS + 4231781 4233430 pgi b4025 CDS + 4233929 4234171 yjbE b4026 CDS + 4234285 4234923 yjbF b4027 CDS + 4234920 4235657 yjbG b4028 CDS + 4235657 4237753 yjbH b4029 CDS + 4238348 4238758 yjbA b4030 CDS − 4238802 4240277 xylE b4031 CDS − 4240649 4241539 malG b4032 CDS − 4241554 4243098 malF b4033 CDS − 4243252 4244442 malE b4034 CDS + 4244807 4245922 malK b4035 CDS + 4245994 4247334 lamB b4036 CDS + 4247577 4248497 malM b4037 CDS + 4248978 4250306 yjbI b4038 CDS + 4250529 4251026 ubiC b4039 CDS + 4251039 4251911 ubiA b4040 CDS − 4252066 4254489 plsB b4041 CDS + 4254660 4255028 dgkA b4042 CDS + 4255138 4255746 lexA b4043 CDS + 4255765 4257144 dinF b4044 CDS + 4257260 4257469 yjbJ b4045 CDS − 4257511 4258026 zur b4046 CDS + 4258344 4258598 yjbL b4047 CDS + 4258622 4259329 yjbM b4048 CDS + 4259692 4260729 yjbN b4049 CDS + 4260863 4261105 yjbO b4050 CDS − 4261271 4262254 qor b4051 CDS + 4262337 4263752 dnaB b4052 CDS + 4263805 4264884 alr b4053 CDS + 4265137 4266330 tyrB b4054 CDS + 4267437 4268150 aphA b4055 CDS + 4268261 4268677 yjbQ b4056 CDS + 4268681 4269037 yjbR b4057 CDS − 4269072 4271894 uvrA b4058 CDS + 4272148 4272684 ssb b4059 CDS − 4272783 4273064 yjcB b4060 CDS + 4273494 4275080 yjcC b4061 CDS − 4275083 4275406 soxS b4062 CDS + 4275492 4275956 soxR b4063 CDS + 4276502 4277851 yjcD b4064 CDS + 4278003 4279652 yjcE b4065 CDS − 4279806 4281098 yjcF b4066 CDS − 4281276 4282925 actP b4067 CDS − 4282922 4283236 yjcH b4068 CDS − 4283436 4285394 acs b4069 CDS + 4285787 4287223 nrfA b4070 CDS + 4287268 4287834 nrf8 b4071 CDS + 4287831 4288502 nrfC b4072 CDS + 4288499 4289455 nrfD b4073 CDS + 4289535 4291193 nrfE b4074 CDS + 4291186 4291569 nrfF b4075 CDS + 4291566 4292162 nrfG b4076 CDS + 4292504 4293817 gltP b4077 CDS − 4294459 4295148 yjcO b4078 CDS − 4295242 4297389 fdhF b4079 CDS − 4297587 4299053 yjcP b4080 CDS − 4299050 4301101 yjcQ b4081 CDS − 4301101 4302132 yjcR b4082 CDS − 4302635 4304620 yjcS b4083 CDS − 4304893 4305822 alsK b4084 CDS − 4305806 4306501 alsE b4085 CDS − 4306512 4307492 alsC b4086 CDS − 4307471 4309003 alsA b4087 CDS − 4309130 4310065 alsB b4088 CDS − 4310124 4311014 rpiR b4089 CDS + 4311373 4311822 rpiB b4090 CDS − 4312367 4313125 phnP b4092 CDS − 4313127 4313561 phnO b4093 CDS − 4313548 4314105 phnN b4094 CDS − 4314105 4315241 phnM b4095 CDS − 4315238 4315918 phnL b4096 CDS − 4316029 4316787 phnK b4097 CDS − 4316784 4317629 phnJ b4098 CDS − 4317622 4318686 phnI b4099 CDS − 4318686 4319270 phnH b4100 CDS − 4319267 4319719 phnG b4101 CDS − 4319720 4320445 phnF b4102 CDS − 4320466 4320834 b4103 CDS − 4320684 4321304 phnE b4104 CDS − 4321359 4322375 phnD b4105 CDS − 4322400 4323188 phnC b4106 CDS − 4323321 4323764 phnB b4107 CDS − 4324422 4324757 phnA b4108 CDS + 4325158 4327386 yjdA b4109 CDS + 4327383 4328261 yjcZ b4110 CDS + 4328525 4330027 proP b4111 CDS − 4330204 4331295 basS b4112 CDS − 4331305 4331973 basR b4113 CDS − 4331970 4333613 yjdB b4114 CDS − 4333717 4335054 yjdE b4115 CDS − 4335191 4335952 adiY b4116 CDS − 4336277 4338547 adiA b4117 CDS − 4338743 4339651 melR b4118 CDS + 4339934 4341289 melA b4119 CDS + 4341404 4342813 melB b4120 CDS − 4342952 4343581 yjdF b4121 CDS − 4343703 4345349 fumB b4122 CDS − 4345427 4346767 dcuB b4123 CDS − 4347338 4348057 dcuR b4124 CDS − 4348054 4349685 dcuS b4125 CDS + 4349866 4350096 yjdI b4126 CDS + 4350108 4350380 yjdJ b4127 CDS + 4350607 4350903 yjdK b4128 CDS − 4351223 4352740 lysU b4129 CDS − 4352977 4354434 yjdL b4130 CDS − 4354493 4356640 cadA b4131 CDS − 4356720 4358054 cadB b4132 CDS − 4358419 4359957 cadC b4133 tRNA − 4360574 4360649 pheU b4134 CDS − 4360756 4361331 yjdC b4135 CDS − 4361368 4363065 dsbD b4136 CDS − 4363041 4363379 cutA b4137 CDS − 4363495 4364796 dcuA b4138 CDS − 4364914 4366350 aspA b4139 CDS + 4366687 4367163 fxsA b4140 CDS − 4367179 4368435 yjeH b4141 CDS + 4368711 4369004 groS b4142 CDS + 4369048 4370694 groL b4143 CDS + 4370832 4371185 yjeI b4144 CDS − 4371388 4372257 yjeJ b4145 CDS − 4372652 4373680 yjeK b4146 CDS + 4373722 4374288 efp b4147 CDS + 4374898 4375215 sugE b4148 CDS − 4375212 4375745 blc b4149 CDS − 4375834 4376967 ampC b4150 CDS − 4377030 4377389 frdD b4151 CDS − 4377400 4377795 frdC b4152 CDS − 4377806 4378540 frdB b4153 CDS − 4378533 4380341 frdA b4154 CDS + 4380666 4381643 yjeA b4155 CDS + 4381862 4383364 yjeM b4156 CDS + 4383416 4383730 yjeN b4157 CDS + 4383727 4384041 yjeo b4158 CDS − 4384070 4387393 yjeP b4159 CDS − 4387415 4388383 psd b4160 CDS − 4388480 4389532 yjeQ b4161 CDS + 4389627 4390172 orn b4162 tRNA + 4390383 4390458 glyV b4163 tRNA + 4390495 4390570 glyX b4164 tRNA + 4390606 4390681 glyY b4165 CDS − 4390951 4392090 yjeS b4166 CDS + 4392089 4393636 yjeF b4167 CDS + 4393608 4394069 yjeE b4168 CDS + 4394088 4395425 amiB b4169 CDS + 4395435 4397282 mutL b4170 CDS + 4397275 4398225 miaA b4171 CDS + 4398311 4398619 hfq b4172 CDS + 4398695 4399975 hflX b4173 CDS + 4400061 4401320 hflK b4174 CDS + 4401323 4402327 hflC b4175 CDS + 4402409 4402606 yjeT b4176 CDS + 4402710 4404008 purA b4177 CDS + 4404213 4404638 yjeB b4178 CDS + 4404677 4407118 rnr b4179 CDS + 4407298 4408029 yjfH b4180 CDS + 4408156 4408557 yjfI b4181 CDS + 4408576 4409274 yjfJ b4182 CDS + 4409325 4409984 yjfK b4183 CDS + 4410002 4410400 yjfL b4184 CDS + 4410410 4411048 yjfM b4185 CDS + 4411051 4412214 yjfC b4186 CDS + 4412298 4413923 aidB b4187 CDS − 4414040 4414315 yjfN b4188 CDS − 4414464 4414793 yjfO b4189 CDS + 4414975 4415724 yjfP b4190 CDS − 4415721 4416476 yjfQ b4191 CDS − 4416584 4417648 yjfR b4192 CDS + 4418003 4419400 sgaT b4193 CDS + 4419416 4419721 sgaB b4194 CDS + 4419731 4420195 ptxA b4195 CDS + 4420209 4420859 sgaH b4196 CDS + 4420869 4421723 sgaU b4197 CDS + 4421723 4422409 sgaE b4198 CDS − 4422539 4422814 yjfY b4199 CDS + 4423141 4423536 rpsF b4200 CDS + 4423543 4423857 priB b4201 CDS + 4423862 4424089 rpsR b4202 CDS + 4424131 4424580 rplI b4203 CDS − 4424651 4425445 yjfZ b4204 CDS + 4425792 4426118 ytfA b4205 CDS − 4426102 4426740 ytfB b4206 CDS + 4426958 4427578 fklB b4207 CDS + 4427887 4429299 cycA b4208 CDS − 4429344 4430006 ytfE b4209 CDS − 4430114 4431088 ytfF b4210 CDS − 4431187 4432047 ytfG b4211 CDS + 4432136 4432516 ytfH b4212 CDS − 4432645 4434588 cpdB b4213 CDS + 4434778 4435518 cysQ b4214 CDS + 4435730 4436668 ytfI b4215 CDS − 4436731 4437285 ytfJ b4216 CDS + 4437610 4437816 ytfK b4217 CDS − 4437895 4439238 ytfL b4218 CDS − 4439561 4440199 msrA b4219 CDS + 4440405 4442138 ytfM b4220 CDS + 4442135 4445914 ytfN b4221 CDS + 4445917 4446258 ytfP b4222 CDS − 4445999 4446268 yzfA b4223 CDS + 4446470 4446721 chpS b4224 CDS + 4446715 4447065 chpB b4225 CDS − 4447145 4447675 ppa b4226 CDS + 4447985 4448941 ytfQ b4227 CDS + 4450597 4451619 ytfT b4230 CDS + 4451606 4452601 yjfF b4231 CDS − 4452634 4453632 fbp b4232 CDS + 4453808 4455181 mpl b4233 CDS − 4455337 4455888 yjgA b4234 CDS + 4455982 4457334 pmbA b4235 CDS + 4457576 4457878 cybC b4236 CDS − 4457923 4458387 nrdG b4237 CDS − 4458545 4460683 nrdD b4238 CDS − 4461077 4462732 treC b4239 CDS − 4462782 4464203 treB b4240 CDS − 4464322 4465269 treR b4241 CDS + 4465648 4468344 mgtA b4242 CDS − 4468550 4468936 yjgF b4243 CDS − 4469009 4469470 pyrI b4244 CDS − 4469483 4470418 pyrB b4245 CDS − 4470422 4470556 pyrL b4246 CDS + 4470536 4470868 yjgG b4247 CDS − 4470837 4471232 yjgH b4248 CDS − 4471363 4472076 yjgI b4249 CDS + 4472250 4472414 b4250 CDS + 4472147 4472740 yjgJ b4251 CDS + 4472885 4473337 yjgK b4252 CDS + 4473460 4475274 yjgL b4253 CDS − 4475330 4476334 argI b4254 CDS + 4476496 4476912 yjgD b4255 CDS − 4477057 4477560 yjgM b4256 CDS + 4477753 4478949 yjgN b4257 CDS − 4479005 4481860 valS b4258 CDS − 4481860 4482303 holC b4259 CDS − 4482463 4483974 pepA b4260 CDS + 4484241 4485341 yjgP b4261 CDS + 4485341 4486423 yjgQ b4262 CDS − 4486584 4488086 yjgR b4263 CDS − 4488164 4489162 idnR b4264 CDS − 4489229 4490548 idnT b4265 CDS − 4490610 4491374 idnO b4266 CDS − 4491398 4492429 idnD b4267 CDS + 4492646 4493209 idnK b4268 CDS − 4493213 4494232 yjgB b4269 tRNA + 4494428 4494512 leuX b4270 CDS + 4494773 4495963 intB b4271 repeat_region − 4496204 4497534 CDS + 4496250 4496660 yi21_6 b4272 CDS + 4496618 4497523 yi22_6 b4273 CDS + 4497622 4497957 yjgW b4274 CDS − 4498066 4498512 yjgX b4275 CDS − 4498455 4498814 yjgY b4276 CDS + 4499283 4499612 yjgZ b4277 repeat_region − 4500113 4501538 CDS − 4500126 4501454 yi41 b4278 CDS + 4502081 4503298 yjhB b4279 CDS + 4503310 4504428 yjhC b4280 CDS − 4504649 4504879 yjhD b4281 CDS + 4504884 4505132 yjhE b4282 repeat_region + 4505138 4505475 CDS + 4505220 4505486 yi91b b4283 repeat_region − 4505482 4506702 CDS − 4505489 4506640 tra8_3 b4284 repeat_region − 4506703 4507029 CDS + 4506981 4507577 b4285 repeat_region + 4507030 4507824 CDS + 4507743 4508156 b4286 CDS − 4508713 4509480 fecE b4287 CDS − 4509481 4510437 fecD b4288 CDS − 4510434 4511432 fecC b4289 CDS − 4511429 4512331 fecB b4290 CDS − 4512376 4514700 fecA b4291 CDS − 4514787 4515740 fecR b4292 CDS − 4515737 4516258 fecl b4293 repeat_region + 4516495 4517262 CDS + 4516550 4516825 insA_7 b4294 CDS − 4517361 4518347 yjhU b4295 CDS − 4518694 4520043 yjhF b4296 CDS − 4520150 4522117 yjhG b4297 CDS − 4522128 4523033 yjhH b4298 CDS − 4523038 4523828 yjhI b4299 CDS − 4524129 4524911 sgcR b4300 CDS − 4524928 4525560 sgcE b4301 CDS − 4525572 4526003 sgcA b4302 CDS − 4526134 4526940 sgcQ b4303 CDS − 4526953 4528266 sgcC b4304 CDS − 4528553 4529674 sgcX b4305 CDS − 4530460 4531206 yjhP b4306 CDS − 4531262 4531807 yjhQ b4307 CDS + 4533038 4534054 yjhR b4308 CDS − 4534637 4535617 yjhS b4309 CDS − 4535682 4536788 yjhT b4310 CDS − 4536808 4537524 yjhA b4311 CDS + 4538980 4539582 fimB b4312 CDS + 4540060 4540656 fimE b4313 CDS + 4541138 4541686 fimA b4314 CDS + 4541751 4542290 fimI b4315 CDS + 4542327 4543052 fimC b4316 CDS + 4543119 4545755 fimD b4317 CDS + 4545765 4546295 fimF b4318 CDS + 4546308 4546811 fimG b4319 CDS + 4546831 4547733 fimH b4320 CDS − 4547976 4549319 gntP b4321 CDS + 4549659 4550843 uxuA b4322 CDS + 4550924 4552384 uxuB b4323 CDS + 4552599 4553372 uxuR b4324 CDS − 4553513 4554343 yjiC b4325 CDS + 4555016 4555408 yjiD b4326 CDS − 4555401 4556312 yjiE b4327 CDS − 4556377 4557549 iadA b4328 CDS − 4557562 4558023 yjiG b4329 CDS − 4558020 4558703 yjiH b4330 CDS + 4558953 4559507 kptA b4331 CDS − 4559520 4560698 yjiJ b4332 CDS − 4560766 4561734 yjiK b4333 CDS − 4561945 4562712 yjiL b4334 CDS − 4562722 4563873 yjiM b4335 CDS − 4563989 4565269 yjiN b4336 CDS − 4565310 4566542 yjiO b4337 CDS + 4567021 4567332 yjiP b4338 CDS + 4567381 4567941 yjiQ b4339 CDS − 4568185 4569597 yjiR b4340 CDS + 4569774 4569938 yjiS b4341 CDS + 4570437 4571954 yjiT b4342 CDS − 4574935 4575981 mcrC b4345 CDS − 4575981 4577360 mcrB b4346 CDS − 4577522 4577920 yjiW b4347 CDS − 4578091 4579485 hsdS b4348 CDS − 4579482 4581071 hsdM b4349 CDS − 4581272 4584838 hsdR b4350 CDS + 4584972 4585886 mrr b4351 CDS − 4585932 4586888 yjiA b4352 CDS − 4586899 4587102 yjiX b4353 CDS − 4587152 4589302 yjiY b4354 CDS + 4589680 4591335 tsr b4355 CDS − 4591384 4592745 yjiZ b4356 CDS − 4592960 4593874 yjjM b4357 CDS + 4594013 4595035 yjjN b4358 CDS − 4595173 4597464 mdoB b4359 CDS − 4597718 4598212 yjjA b4360 CDS − 4598261 4598998 dnaC b4361 CDS − 4599001 4599540 dnaT b4362 CDS − 4599647 4599973 yjjB b4363 CDS − 4600111 4600881 yjjP b4364 CDS + 4601500 4602225 yjjQ b4365 CDS + 4602237 4602860 bglJ b4366 CDS − 4602898 4603686 fhuF b4367 tRNA − 4604102 4604188 leuV b4368 tRNA − 4604223 4604309 leuP b4369 tRNA − 4604338 4604424 leuQ b4370 CDS − 4604692 4605723 rsmC b4371 CDS + 4605826 4606239 holD b4372 CDS + 4606208 4606654 rimI b4373 CDS + 4606669 4607346 yjjG b4374 CDS + 4607437 4609026 prfC b4375 CDS + 4609419 4610024 osmY b4376 CDS + 4610434 4611507 yjjU b4377 CDS + 4611504 4612283 yjjV b4378 CDS − 4612703 4613566 yjjW b4379 CDS − 4613538 4615088 yjjI b4380 CDS + 4615346 4616125 deoC b4381 CDS + 4616252 4617574 deoA b4382 CDS + 4617626 4618849 deoB b4383 CDS + 4618906 4619625 deoD b4384 CDS + 4619792 4621123 yjjJ b4385 CDS − 4621124 4622140 IplA b4386 CDS − 4622168 4622812 smp b4387 CDS + 4622918 4623886 serB b4388 CDS + 4623935 4625317 sms b4389 CDS + 4625338 4626570 nadR b4390 CDS − 4626878 4628545 yjjK b4391 CDS + 4628756 4630693 slt b4392 CDS + 4630783 4631109 trpR b4393 CDS − 4631256 4631768 yjjX b4394 CDS + 4631820 4632467 gpmB b4395 CDS − 4632464 4633333 rob b4396 CDS + 4633544 4634017 creA b4397 CDS + 4634030 4634719 creB b4398 CDS + 4634719 4636143 creC b4399 CDS + 4636201 4637553 creD b4400 CDS − 4637613 4638329 arcA b4401 CDS + 4638425 4638565 yjjY b4402 CDS + 4638965 4639651 lasT b4403 CDS − 4190660 4190860 thiS b4407 CDS + 4374340 4374465 ecnA b4410 CDS + 4374576 4374722 ecnB b4411 CDS + 1702575 1702700 blr b4409 misc_RNA − 2922178 2922537 csrB b4408 CDS − 213925 214125 yaeP b4406 misc_RNA + 16952 17006 sokC b4413 misc_RNA + 189712 189847 t44 b4414 misc_RNA − 852175 852263 rybA b4416 misc_RNA − 887199 887277 rybB b4417 misc_RNA + 1145812 1145980 sraB b4418 misc_RNA + 1268546 1268612 rdlA b4420 misc_RNA + 1269081 1269146 rdlB b4422 misc_RNA + 1269616 1269683 rdlC b4424 misc_RNA − 1286289 1286459 rtT b4425 misc_RNA − 1403676 1403833 IS061 b4426 misc_RNA + 1435145 1435253 micC b4427 misc_RNA + 1490143 1490195 sokB b4429 misc_RNA − 1762737 1762804 rydB b4430 misc_RNA + 1768396 1768500 rprA b4431 misc_RNA + 1921090 1921338 ryeA b4432 misc_RNA − 1921188 1921308 ryeB b4433 misc_RNA − 1985863 1986022 IS092 b4434 misc_RNA + 2069339 2069542 IS102 b4435 misc_RNA + 2151299 2151447 ryeC b4436 misc_RNA + 2151634 2151776 ryeD b4437 misc_RNA + 2165136 2165221 ryeE b4438 misc_RNA + 2311106 2311198 micF b4439 misc_RNA + 2651877 2652180 ryfA b4440 misc_RNA − 2689214 2689362 tke1 b4441 misc_RNA + 2812823 2812897 sraD b4442 misc_RNA + 2940718 2940922 gcvB b4443 misc_RNA − 2974124 2974211 rygA b4444 misc_RNA − 2974332 2974407 rygB b4445 misc_RNA + 3054837 3054987 rygC b4446 misc_RNA − 3192773 3192922 rygD b4447 misc_RNA + 3236396 3236583 sraF b4448 misc_RNA + 3309247 3309420 sraG b4449 misc_RNA + 3348599 3348706 ryhA b4450 misc_RNA − 3578946 3579039 ryhB b4451 misc_RNA + 3662887 3662991 IS183 b4452 misc_RNA + 3698159 3698222 rdlD b4454 misc_RNA + 3984455 3984626 ryiA b4456 misc_RNA + 4049059 4049303 csrC b4457 misc_RNA − 4156308 4156417 oxyS b4458 misc_RNA − 4275950 4276089 ryjA b4459 CDS − 16751 16903 hokC b4412 CDS + 607059 607211 hokE b4415 CDS − 1268391 1268498 ldrA b4419 CDS − 1268926 1269033 ldrB b4421 CDS − 1269461 1269568 ldrC b4423 CDS − 1489946 1490095 hokB b4428 CDS − 3698003 3698110 ldrD b4453 CDS − 3718471 3718623 hokA b4455 misc_feature − 1588774 1588801 CDS − 1980578 1981567 araH b4460 CDS − 2895986 2897440 b4463 CDS − 3074201 3075478 yggP b4465 CDS − 3112572 3117134 yghJ b4466 CDS − 3122258 3123481 glcF b4467 CDS − 3123492 3124544 glcE b4468 CDS − 3156949 3159168 ygiQ b4469 CDS − 3253363 3254673 yhaM b4470 CDS − 3256307 3257671 tdcG b4471 CDS − 3390480 3394280 yhdP b4472 CDS − 3430458 3431582 smf b4473 CDS − 3553855 3554871 rtcA b4475 CDS − 3573744 3575084 gntU b4476 CDS − 3869873 3871021 dgoD b4478 CDS − 3871018 3871635 dgoA b4477 CDS − 3872494 3873183 dgoR b4479 CDS − 3945151 3945990 hdfR b4480 CDS − 3998315 3999079 yigE b4482 CDS + 2746886 2748082 yfjD b4461 CDS + 2784770 2785456 ygaR b4462 CDS + 3025143 3026510 ygfQ b4464 CDS + 3500362 3501192 frlC b4474 CDS + 3975548 3976627 rffT b4481 CDS + 4021577 4022359 tatD b4483 CDS + 4103843 4104343 cpxP b4484 CDS + 4449081 4450583 ytfR b4485 CDS + 4572158 4574878 yjiV b4486 CDS + 4311891 4312220 yjdP b4487 CDS + 3948583 3950227 ilvG b4488 prophage + 262182 296489 prophage + 564025 585326 prophage + 1195443 1210646 prophage + 1409949 1433008 prophage + 1630310 1650778 prophage + 2064183 2077055 prophage + 2464407 2474621 prophage + 2556721 2563510 prophage + 2753971 2776001 prophage + 4494563 4534632 CDS + 2474332 2474532 torI b4501 CDS − 2263063 2263317 yeiW b4502

TABLE 2 Strain Type Coordinates Name B Function MDS01 CDS complement(262552 . . . 262893) b0245 orf, hypothetical protein MDS01 CDS complement(262914 . . . 263231) yafW b0246 orf, hypothetical protein MDS01 CDS complement(263480 . . . 263956) ykfG b0247 putative DNA repair protein MDS01 CDS complement(263972 . . . 264430) yafX b0248 orf, hypothetical protein MDS01 CDS complement(264528 . . . 264767) ykfF b0249 orf, hypothetical protein MDS01 CDS complement(264844 . . . 265311) ykfB b0250 orf, hypothetical protein MDS01 CDS complement(265334 . . . 266191) yafY b0251 hypothetical transcriptional regulator MDS01 CDS complement(266408 . . . 267244) yafZ b0252 orf, hypothetical protein MDS01 CDS complement(267321 . . . 268187) ykfA b0253 putative GTP-binding protein MDS01 CDS complement(268513 . . . 269406) perR b0254 peroxide resistance protein MDS01 CDS 269466 . . . 269870 yi91a b0255 MDS01 CDS 269827 . . . 270978 tra8_1 b0256 IS30 transposase MDS01 CDS 271054 . . . 271479 b0257 putative transposase MDS01 CDS 272086 . . . 273216 ykfC b0258 orf, hypothetical protein MDS01 CDS complement(273325 . . . 274341) trs5_1 b0259 IS5 transposase MDS01 CDS 274525 . . . 275952 ykfB b0260 orf, hypothetical protein MDS01 CDS 275939 . . . 276871 yafY b0261 hypothetical transcriptional regulator MDS01 CDS complement(276980 . . . 278038) afuC b0262 putative ATP-binding component of a transport system MDS01 CDS complement(278038 . . . 278400) b0263 MDS01 CDS complement(278402 . . . 278905) insB_2 b0264 IS1 protein InsB MDS01 CDS complement(278824 . . . 279099) insA_2 b0265 IS1 protein InsA MDS01 CDS complement(279609 . . . 279986) yagB b0266 orf, hypothetical protein MDS01 CDS complement(280053 . . . 281207) yagA b0267 orf, hypothetical protein MDS01 CDS 281481 . . . 282410 yagE b0268 putative lyase/synthase MDS01 CDS 282425 . . . 284392 yagF b0269 putative dehydratase MDS01 CDS 284619 . . . 286001 yagG b0270 putative permease MDS01 CDS 286013 . . . 287623 yagH b0271 putative beta-xylosidase (EC 3.2.1.37 MDS01 CDS complement(287628 . . . 288386) yagI b0272 putative regulator MDS01 CDS complement(288525 . . . 289529) argF b0273 ornithine carbamoyltransferase 2, chain F MDS01 CDS complement(289873 . . . 290376) insB_3 b0274 IS1 protein InsB MDS01 CDS complement(290295 . . . 290570) insA_3 b0275 IS1 protein InsA MDS01 CDS 290724 . . . 291455 yagJ b0276 orf, hypothetical protein MDS01 CDS complement(291546 . . . 292172) yagK b0277 orf, hypothetical protein MDS01 CDS complement(292444 . . . 293142) yagL b0278 DNA-binding protein MDS01 CDS complement(293169 . . . 294023) yagM b0279 orf, hypothetical protein MDS01 CDS complement(294363 . . . 294803) yagN b0280 orf, hypothetical protein MDS01 CDS complement(294920 . . . 296320) intF b0281 putative phage integrase MDS01 CDS complement(296605 . . . 297015) yagP b0282 putative transcriptional regulator LYSR-type MDS01 CDS complement(296994 . . . 297950) yagQ b0283 orf, hypothetical protein MDS01 CDS complement(297960 . . . 300158) yagR b0284 orf, hypothetical protein MDS01 CDS complement(300155 . . . 301111) yagS b0285 orf, hypothetical protein MDS01 CDS complement(301108 . . . 301797) yagT b0286 putative xanthine dehydrogenase (EC 1.1.1.20 MDS01 CDS 302215 . . . 302829 yagU b0287 orf, hypothetical protein MDS01 CDS complement(303077 . . . 303406) ykgJ b0288 putative ferredoxin MDS01 CDS complement(303719 . . . 304474) yagV b0289 orf, hypothetical protein MDS01 CDS complement(304398 . . . 306041) yagW b0290 putative receptor MDS01 CDS complement(306031 . . . 308556) yagX b0291 putative enzyme MDS01 CDS complement(308582 . . . 309250) yagY b0292 orf, hypothetical protein MDS01 CDS complement(309308 . . . 309895) yagZ b0293 orf, hypothetical protein MDS01 CDS complement(309970 . . . 310560) ykgK b0294 putative regulator MDS01 CDS 311336 . . . 311563 ykgL b0295 orf, hypothetical protein MDS01 CDS complement(311738 . . . 312001) ykgM b0296 putative ribosomal protein MDS01 CDS 313581 . . . 314468 eaeH b0297 attaching and effacing protein, pathogenesis factor MDS01 CDS 314506 . . . 314814 b0298 MDS01 CDS 314811 . . . 315677 tra5_5 b0299 MDS01 CDS complement(315674 . . . 316393) ykgA b0300 putative ARAC-type regulatory protein MDS01 CDS complement(316950 . . . 317552) ykgB b0301 orf, hypothetical protein MDS01 CDS complement(317555 . . . 317806) ykgI b0303 orf, hypothetical protein MDS01 CDS complement(317900 . . . 319252) ykgC b0304 putative oxidoreductase MDS01 CDS 319451 . . . 320305 ykgD b0305 putative ARAC-type regulatory protein MDS01 CDS 320832 . . . 321551 ykgE b0306 putative dehydrogenase subunit MDS01 CDS 321562 . . . 322989 ykgF b0307 orf, hypothetical protein MDS01 CDS 322829 . . . 323677 ykgG b0308 putative transporter MDS01 CDS complement(323632 . . . 323844) b0309 orf, hypothetical protein MDS01 CDS complement(323920 . . . 324588) ykgH b0310 orf, hypothetical protein MDS02 CDS complement(1398271 . . . 1399803) ydaH b1336 MDS02 CDS complement(1399834 . . . 1401279) b1337 MDS02 CDS complement(1401279 . . . 1402604) ydaJ b1338 MDS02 CDS 1402765 . . . 1403673 ydaK b1339 putative transcriptional regulator LYSR-type MDS02 CDS 1404003 . . . 1404566 ydaL b1340 orf, hypothetical protein MDS02 CDS complement(1404587 . . . 1405879) b1341 orf, hypothetical protein MDS02 CDS 1406074 . . . 1407057 b1342 orf, hypothetical protein MDS02 CDS 1407535 . . . 1408908 dbpA b1343 ATP-dependent RNA helicase MDS02 CDS complement(1409037 . . . 1409972) ydaO b1344 orf, hypothetical protein MDS02 CDS complement(1410024 . . . 1411259) b1345 MDS02 CDS complement(1411261 . . . 1411500) ydaQ b1346 putative lambdoid prophage Rac excisionase MDS02 CDS complement(1411555 . . . 1411764) ydaC b1347 orf, hypothetical protein MDS02 CDS complement(1411757 . . . 1411951) lar b1348 restriction alleviation and modification enhancement MDS02 CDS complement(1412008 . . . 1412817) recT b1349 recombinase, DNA renaturation MDS02 CDS complement(1412810 . . . 1415410) recE b1350 exonuclease VIII, ds DNA exonuclease 5′ --> 3′ specific MDS02 CDS complement(1415512 . . . 1415787) racC b1351 RacC protein MDS02 CDS complement(1416032 . . . 1416265) kil b1352 Kil protein (killing function) of lambdoid prophage Rac MDS02 CDS 1416572 . . . 1417183 sieB b1353 phage superinfection exclusion protein MDS02 CDS 1417192 . . . 1417368 b1354 orf, hypothetical protein MDS02 CDS complement(1417346 . . . 1417525) b1355 orf, hypothetical protein MDS02 CDS complement(1417789 . . . 1418265) ydaR b1356 MDS02 CDS 1418389 . . . 1418685 ydaS b1357 orf, hypothetical protein MDS02 CDS 1418708 . . . 1419130 ydaT b1358 orf, hypothetical protein MDS02 CDS 1419143 . . . 1420000 ydaU b1359 orf, hypothetical protein MDS02 CDS 1420007 . . . 1420753 b1360 putative DNA replication factor MDS02 CDS 1420725 . . . 1421336 ydaW b1361 orf, hypothetical protein MDS02 CDS 1421363 . . . 1421668 b1362 putative Rac prophage endopeptidase MDS02 CDS 1421806 . . . 1423263 trkG b1363 MDS02 CDS 1423202 . . . 1423483 b1364 orf, hypothetical protein MDS02 CDS 1423401 . . . 1423664 b1365 orf, hypothetical protein MDS02 CDS 1423645 . . . 1424004 ydaY b1366 orf, hypothetical protein MDS02 CDS 1424079 . . . 1424312 b1367 orf, hypothetical protein MDS02 CDS 1424478 . . . 1425506 b1368 putative alpha helix protein MDS02 CDS 1425482 . . . 1425637 b1369 orf, hypothetical protein MDS02 CDS complement(1425770 . . . 1426750) trs5_5 b1370 MDS02 CDS 1426547 . . . 1427008 b1371 orf, hypothetical protein MDS02 CDS 1427067 . . . 1430435 b1372 MDS02 CDS 1430435 . . . 1431010 b1373 tail fiber assembly protein homolog from lambdoid prophage Rac MDS02 CDS complement(1431108 . . . 1431698) b1374 MDS02 CDS complement(1432015 . . . 1432281) ynaE b1375 orf, hypothetical protein MDS02 CDS complement(1433209 . . . 1433715) b1376 ynaF putative filament protein MDS02 CDS complement(1433784 . . . 1434917) b1377 MDS02 CDS complement(1435284 . . . 1438808) ydbK b1378 putative oxidoreductase, Fe—S subunit MDS02 CDS complement(1439345 . . . 1439767) hslJ b1379 heat shock protein HslJ MDS02 CDS complement(1439878 . . . 1440867) ldhA b1380 fermentative D-lactate dehydrogenase NAD-dependent MDS02 CDS 1441075 . . . 1443714 ydbH b1381 orf, hypothetical protein MDS02 CDS 1443711 . . . 1443896 ynbE b1382 orf, hypothetical protein MDS02 CDS 1443898 . . . 1444230 ydbL b1383 orf, hypothetical protein MDS02 CDS complement(1444402 . . . 1445307) feaR b1384 regulatory protein for 2- phenylethylamine catabolism MDS02 CDS 1445540 . . . 1447042 feaB b1385 phenylacetaldehyde dehydrogenase MDS02 CDS complement(1447100 . . . 1449373) tynA b1386 copper amine oxidase (tyramine oxidase) MDS02 CDS complement(1449621 . . . 1451666) maoC b1387 MDS02 CDS 1451951 . . . 1452880 ydbO b1388 MDS02 CDS 1452892 . . . 1453179 ynbF b1389 MDS02 CDS 1453188 . . . 1453934 ydbP b1390 MDS02 CDS 1453943 . . . 1454446 b1391 MDS02 CDS 1454454 . . . 1455524 b1392 MDS02 CDS 1455521 . . . 1456288 ydbS b1393 MDS02 CDS 1456288 . . . 1457076 b1394 MDS02 CDS 1457078 . . . 1458505 ydbU b1395 MDS02 CDS 1458495 . . . 1458917 b1396 MDS02 CDS 1458917 . . . 1460122 b1397 MDS02 CDS 1460149 . . . 1461462 b1398 MDS02 CDS 1461563 . . . 1462513 b1399 MDS02 CDS 1462495 . . . 1463085 b1400 MDS02 CDS 1463416 . . . 1465974 ydbA_1 b1401 MDS02 CDS complement(1465945 . . . 1466850) yi22_2 b1402 IS2 hypothetical protein MDS02 CDS complement(1466808 . . . 1467218) yi21_2 b1403 IS2 hypothetical protein MDS02 CDS 1467382 . . . 1468533 tra8_2 b1404 IS30 transposase MDS02 CDS 1468714 . . . 1472037 ydbA_2 b1405 MDS02 CDS 1472245 . . . 1473105 ydbC b1406 putative dehydrogenase MDS02 CDS 1473162 . . . 1475474 ydbD b1407 orf, hypothetical protein MDS02 CDS 1475639 . . . 1476250 b1408 MDS02 CDS 1476250 . . . 1477146 b1409 putative phosphatidate cytidiltransferase MDS02 CDS 1477162 . . . 1478919 b1410 orf, hypothetical protein MDS02 CDS 1478933 . . . 1480225 ynbD b1411 putative enzymes MDS02 misc_RNA complement(1403676 . . . 1403833) IS061 b4426 MDS02 misc_RNA 1435145 . . . 1435252 tke8 b4427 MDS03 CDS complement(2555340 . . . 2556743) eutB b2441 ethanolamine ammonia-lyase, heavy chain MDS03 CDS 2556793 . . . 2558088 b2442 putative prophage integrase MDS03 CDS 2558279 . . . 2558920 b2443 orf, hypothetical protein MDS03 CDS 2559390 . . . 2559635 b2444 orf, hypothetical protein MDS03 CDS 2559632 . . . 2560015 b2445 orf, hypothetical protein MDS03 CDS 2560133 . . . 2560549 b2446 orf, hypothetical protein MDS03 CDS 2560546 . . . 2561139 b2447 orf, hypothetical protein MDS03 CDS 2561599 . . . 2561991 b2448 orf, hypothetical protein MDS03 CDS 2562002 . . . 2562394 b2449 orf, hypothetical protein MDS03 CDS 2562515 . . . 2563354 b2450 orf, hypothetical protein MDS04 CDS 2754181 . . . 2755422 intA b2622 prophage CP4-57 integrase MDS04 CDS complement(2755666 . . . 2756622) yfjH b2623 putative histone MDS04 CDS 2756666 . . . 2756878 alpA b2624 prophage CP4-57 regulatory protein alpA MDS04 CDS 2757007 . . . 2758416 yfjI b2625 orf, hypothetical protein MDS04 CDS 2758569 . . . 2759195 yfjJ b2626 orf, hypothetical protein MDS04 CDS complement(2759373 . . . 2761562) yfjK b2627 orf, hypothetical protein MDS04 CDS complement(2761559 . . . 2763175) yfjL b2628 orf, hypothetical protein MDS04 CDS complement(2763535 . . . 2763798) yfjM b2629 orf, hypothetical protein MDS04 CDS 2763940 . . . 2765013 yfjN b2630 putative cell division protein MDS04 CDS 2765057 . . . 2765377 yfjO b2631 orf, hypothetical protein MDS04 CDS 2765732 . . . 2766595 yfjP b2632 putative GTP-binding protein MDS04 CDS 2766687 . . . 2767508 yfjQ b2633 orf, hypothetical protein MDS04 CDS 2767725 . . . 2768426 yfjR b2634 orf, hypothetical protein MDS04 CDS 2768311 . . . 2768703 b2635 orf, hypothetical protein MDS04 CDS 2768454 . . . 2769146 b2636 orf, hypothetical protein MDS04 CDS 2769170 . . . 2769637 yfjT b2637 orf, hypothetical protein MDS04 CDS complement(2769862 . . . 2770176) b2638 orf, hypothetical protein MDS04 CDS complement(2770189 . . . 2770707) b2639 putative pump protein MDS04 CDS complement(2770858 . . . 2771058) b2640 orf, hypothetical protein MDS04 CDS complement(2770998 . . . 2771114) b2641 orf, hypothetical protein MDS04 CDS 2771340 . . . 2773043 yfjW b2642 orf, hypothetical protein MDS04 CDS 2773941 . . . 2774399 yfjX b2643 orf, hypothetical protein MDS04 CDS 2774408 . . . 2774890 yfjY b2644 putative DNA repair protein MDS04 CDS 2775137 . . . 2775454 yfjZ b2645 orf, hypothetical protein MDS04 CDS 2775475 . . . 2775804 ypjF b2646 orf, hypothetical protein MDS04 CDS complement(2776168 . . . 2780877) ypjA b2647 putative ATP-binding component of a transport system MDS04 CDS complement(2781087 . . . 2781230) b2648 orf, hypothetical protein MDS04 CDS complement(2781660 . . . 2782451) b2649 orf, hypothetical protein MDS04 CDS complement(2782551 . . . 2783033) b2650 orf, hypothetical protein MDS04 CDS 2783243 . . . 2783374 b2651 orf, hypothetical protein MDS04 tRNA complement(2783784 . . . 2783859) ileY b2652 tRNA-Ile MDS04 CDS complement(2783822 . . . 2783995) b2653 orf, hypothetical protein MDS04 CDS 2784419 . . . 2784751 b2654 orf, hypothetical protein MDS04 CDS 2785628 . . . 2786260 b2657 putative enzyme MDS04 CDS 2786399 . . . 2786671 b2658 orf, hypothetical protein MDS04 CDS 2786902 . . . 2787984 b2659 orf, hypothetical protein MDS04 CDS 2787938 . . . 2789272 ygaF b2660 orf, hypothetical protein MDS04 CDS 2784770 . . . 2785456 ygaR b4462 orf, hypothetical protein MDS05 CDS complement(2064329 . . . 2065345) trs5_6 b1994 IS5 transposase MDS05 CDS 2066632 . . . 2067051 b1995 orf, hypothetical protein MDS05 CDS complement(2066976 . . . 2067881) yi22_3 b1996 IS2 hypothetical protein MDS05 CDS complement(2067839 . . . 2068249) yi21_3 b1997 IS2 hypothetical protein MDS05 CDS 2068268 . . . 2068528 b1998 orf, hypothetical protein MDS05 CDS 2068525 . . . 2069235 yeeP b1999 putative histone MDS05 CDS 2069563 . . . 2072682 flu b2000 antigen 43, phase-variable bipartite outer membrane fluffing protein MDS05 CDS 2072797 . . . 2074335 b2001 orf, hypothetical protein MDS05 CDS 2074332 . . . 2074778 yeeS b2002 putative DNA repair protein, RADC family MDS05 CDS 2074841 . . . 2075062 yeeT b2003 orf, hypothetical protein MDS05 CDS 2075136 . . . 2075504 yeeU b2004 putative structural protein MDS05 CDS 2075593 . . . 2075967 yeeV b2005 orf, hypothetical protein MDS05 CDS 2075964 . . . 2076158 yeeW b2006 orf, hypothetical protein MDS05 CDS complement(2077056 . . . 2077451) yeeX b2007 putative alpha helix protein MDS05 CDS complement(2077557 . . . 2078615) yeeA b2008 orf, hypothetical protein MDS05 misc_RNA 2069339 . . . 2069542 b4435 IS102 MDS06 CDS complement(3451530 . . . 3451949) b3322 calcium-binding protein required for initiation of chromosome replication MDS06 CDS complement(3451951 . . . 3453420) yheD b3323 putative export protein A for general secretion pathway (GSP) MDS06 CDS 3453600 . . . 3454415 yheE b3324 MDS06 CDS 3454387 . . . 3456351 yheF b3325 MDS06 CDS 3456361 . . . 3457842 yheG b3326 MDS06 CDS 3457839 . . . 3459035 hofF b3327 MDS06 CDS 3459045 . . . 3459482 hofG b3328 MDS06 CDS 3459490 . . . 3459999 hofH b3329 MDS06 CDS 3459957 . . . 3460373 yheH b3330 MDS06 CDS 3460366 . . . 3460953 yheI b3331 MDS06 CDS 3460946 . . . 3461929 yheJ b3332 MDS06 CDS 3461941 . . . 3463107 yheK b3333 MDS06 CDS 3463080 . . . 3463565 pshM b3334 MDS06 CDS 3463565 . . . 3464242 hofD b3335 MDS06 CDS complement(3464271 . . . 3464747) bfr b3336 bacterioferrin, an iron storage homoprotein MDS06 CDS complement(3464819 . . . 3465013) yheA b3337 MDS06 CDS complement(3465182 . . . 3467875) yheB b3338 MDS07 CDS 2464567 . . . 2465724 intC b2349 MDS07 CDS 2465877 . . . 2466239 b2350 MDS07 CDS 2471542 . . . 2471988 b2350 orf, hypothetical protein MDS07 CDS 2466236 . . . 2467156 b2351 MDS07 CDS 2467153 . . . 2468484 b2352 putative ligase MDS07 CDS 2468783 . . . 2469127 b2353 MDS07 CDS complement(2469099 . . . 2469539) b2354 orf, hypothetical protein MDS07 CDS complement(2469566 . . . 2470084) yfdL b2355 putative RNA polymerase beta MDS07 CDS complement(2470134 . . . 2470442) yfdM b2356 orf, hypothetical protein MDS07 CDS complement(2470409 . . . 2470903) yfdN b2357 orf, hypothetical protein MDS07 CDS complement(2470900 . . . 2471268) yfdO b2358 orf, hypothetical protein MDS07 CDS 2472054 . . . 2472878 b2360 orf, hypothetical protein MDS07 CDS 2472979 . . . 2473542 b2361 orf, hypothetical protein MDS07 CDS 2473533 . . . 2473895 b2362 orf, hypothetical protein MDS07 CDS 2473895 . . . 2474200 b2363 orf, hypothetical protein MDS08 CDS 1625541 . . . 1626287 ydfG b1539 putative oxidoreductase MDS08 CDS 1626376 . . . 1627062 ydfH b1540 orf, hypothetical protein MDS08 CDS 1627239 . . . 1627442 b1541 orf, hypothetical protein MDS08 CDS complement(1627477 . . . 1628937) ydfI b1542 putative oxidoreductase MDS08 CDS complement(1629026 . . . 1630309) b1543 putative transport protein MDS08 CDS 1631063 . . . 1631329 ydfK b1544 orf, hypothetical protein MDS08 CDS 1631646 . . . 1632236 b1545 MDS08 CDS complement(1632334 . . . 1632909) ydfM b1546 tail fiber assembly protein homolog from lambdoid prophage Qin MDS08 CDS complement(1632909 . . . 1633871) b1547 MDS08 CDS complement(1633822 . . . 1634391) nohA b1548 DNA packaging protein NU1 homolog from lambdoid prophages MDS08 CDS 1635056 . . . 1635481 ydfO b1549 orf, hypothetical protein MDS08 CDS complement(1635633 . . . 1635809) b1550 MDS08 CDS complement(1635978 . . . 1636169) b1551 orf, hypothetical protein MDS08 CDS complement(1636479 . . . 1636691) cspI b1552 cold shock-like protein MDS08 CDS complement(1637054 . . . 1637551) b1553 orf, hypothetical protein MDS08 CDS complement(1637548 . . . 1638081) b1554 MDS08 CDS complement(1638078 . . . 1638389) b1555 orf, hypothetical protein MDS08 CDS complement(1638394 . . . 1638684) b1556 MDS08 CDS complement(1639363 . . . 1639578) cspB b1557 MDS08 CDS 1639879 . . . 1640091 cspF b1558 MDS08 CDS complement(1640513 . . . 1641295) b1559 MDS08 CDS complement(1641279 . . . 1642367) b1560 orf, hypothetical protein MDS08 CDS complement(1642675 . . . 1642926) rem b1561 orf, hypothetical protein MDS08 CDS complement(1643143 . . . 1643298) hokD b1562 polypeptide destructive to membrane potential MDS08 CDS complement(1643370 . . . 1643657) relE b1563 orf, hypothetical protein MDS08 CDS complement(1643657 . . . 1643896) relB b1564 negative regulator of translation MDS08 CDS 1643921 . . . 1644226 b1565 orf, hypothetical protein MDS08 CDS 1644429 . . . 1644761 flxA b1566 orf, hypothetical protein MDS08 CDS complement(1645198 . . . 1645347) b1567 orf, hypothetical protein MDS08 CDS complement(1645370 . . . 1645660) b1568 orf, hypothetical protein MDS08 CDS complement(1645644 . . . 1645874) dicC b1569 regulator of dicB MDS08 CDS 1645958 . . . 1646365 dicA b1570 regulator of dicB MDS08 CDS 1646532 . . . 1646687 ydfA b1571 orf, hypothetical protein MDS08 CDS 1646647 . . . 1646817 ydfB b1572 orf, hypothetical protein MDS08 CDS 1646847 . . . 1647065 ydfC b1573 orf, hypothetical protein MDS08 misc_RNA 1647406 . . . 1647458 dicF b1574 DicF antisense RNA, inhibits ftsZ translation MDS08 CDS 1647633 . . . 1647821 dicB b1575 inhibition of cell division MDS08 CDS 1647818 . . . 1648009 ydfD b1576 orf, hypothetical protein MDS08 CDS 1648102 . . . 1649022 ydfE b1577 orf, hypothetical protein MDS08 CDS 1648905 . . . 1649561 b1578 orf, hypothetical protein MDS08 CDS 1649536 . . . 1650732 b1579 MDS09 CDS complement(4493213 . . . 4494274) yjgB b4269 putative oxidoreductase MDS09 tRNA 4494428 . . . 4494512 leuX b4270 tRNA-Leu MDS09 CDS 4494773 . . . 4495963 intB b4271 prophage P4 integrase MDS09 CDS 4496250 . . . 4496660 yi21_6 b4272 IS2 hypothetical protein MDS09 CDS 4496618 . . . 4497523 yi22_6 b4273 IS2 hypothetical protein MDS09 CDS 4497622 . . . 4497957 yjgW b4274 orf, hypothetical protein MDS09 CDS complement(4498066 . . . 4498512) yjgX b4275 orf, hypothetical protein MDS09 CDS complement(4498455 . . . 4498904) yjgY b4276 orf, hypothetical protein MDS09 CDS 4499283 . . . 4499612 yjgZ b4277 orf, hypothetical protein MDS09 CDS complement(4500126 . . . 4501454) yi41 b4278 IS4 hypothetical protein MDS09 CDS 4502021 . . . 4503298 yjhB b4279 putative transport protein MDS09 CDS 4503295 . . . 4504428 yjhC b4280 putative dehydrogenase MDS09 CDS complement(4504649 . . . 4505023) yjhD b4281 orf, hypothetical protein MDS09 CDS 4504929 . . . 4505132 yjhE b4282 orf, hypothetical protein MDS09 CDS 4505184 . . . 4505486 yi91b b4283 MDS09 CDS complement(4505489 . . . 4506640) tra8_3 b4284 IS30 transposase MDS09 CDS 4506981 . . . 4507577 b4285 putative transposase MDS09 CDS 4507743 . . . 4508156 b4286 orf, hypothetical protein MDS09 CDS complement(4508713 . . . 4509480) fecE b4287 ATP-binding component of citrate- dependent MDS09 CDS complement(4509481 . . . 4510437) fecD b4288 citrate-dependent iron transport, membrane-bound protein MDS09 CDS complement(4510434 . . . 4511432) fecC b4289 citrate-dependent iron(III) transport protein, cytosolic MDS09 CDS complement(4511429 . . . 4512337) fecB b4290 citrate-dependent iron transport, periplasmic protein MDS09 CDS complement(4512376 . . . 4514700) fecA b4291 outer membrane receptor; citrate- dependent iron MDS09 CDS complement(4514787 . . . 4515740) fecR b4292 outer membrane receptor; citrate- dependent iron transport, outer membrane receptor MDS09 CDS complement(4515737 . . . 4516258) fecI b4293 probable RNA polymerase sigma factor MDS09 CDS 4516550 . . . 4516825 insA_7 b4294 IS1 protein InsA MDS09 CDS complement(4517361 . . . 4518161) yjhU b4295 orf, hypothetical protein MDS09 CDS complement(4518694 . . . 4520043) yjhF b4296 putative transport system permease MDS09 CDS complement(4520150 . . . 4522117) yjhG b4297 putative dehydratase MDS09 CDS complement(4522128 . . . 4523087) yjhH b4298 putative lyase/synthase MDS09 CDS complement(4523038 . . . 4523826) yjhI b4299 putative regulator MDS09 CDS complement(4524129 . . . 4524911) sgcR b4300 putative DEOR-type transcriptional regulator MDS09 CDS complement(4524928 . . . 4525560) sgcE b4301 putative epimerase MDS09 CDS complement(4525572 . . . 4526003) sgcA b4302 putative PTS system enzyme II A component MDS09 CDS complement(4526134 . . . 4526940) sgcQ b4303 putative nucleoside triphosphatase MDS09 CDS complement(4526953 . . . 4528266) sgcC b4304 putative PTS system enzyme IIC component MDS09 CDS complement(4528553 . . . 4529704) sgcX b4305 putative lyase/synthase MDS09 CDS complement(4530460 . . . 4531206) yjhP b4306 putative methyltransferase MDS09 CDS complement(4531262 . . . 4531807) yjhQ b4307 orf, hypothetical protein MDS09 CDS 4533038 . . . 4534054 yjhR b4308 putative frameshift suppressor″ MDS09 CDS complement(4534637 . . . 4535617) yjhS b4309 orf, hypothetical protein MDS09 CDS complement(4535682 . . . 4536896) yjhT b4310 orf, hypothetical protein MDS09 CDS complement(4536808 . . . 4537533) yjhA b4311 orf, hypothetical protein MDS09 CDS 4538980 . . . 4539582 fimB b4312 recombinase involved in phase variation; regulator for fimA″ MDS09 CDS 4540060 . . . 4540656 fimE b4313 recombinase involved in phase variation; regulator for fimA″ MDS09 CDS 4541138 . . . 4541686 fimA b4314 major type 1 subunit fimbrin (pilin) MDS09 CDS 4541643 . . . 4542290 fimI b4315 fimbrial protein MDS09 CDS 4542327 . . . 4543052 fimC b4316 periplasmic chaperone, required for type 1 fimbriae MDS09 CDS 4543119 . . . 4545755 fimD b4317 outer membrane protein; export and assembly of type 1 fimbriae, interrupted MDS09 CDS 4545765 . . . 4546295 fimF b4318 fimbrial morphology MDS09 CDS 4546308 . . . 4546811 fimG b4319 fimbrial morphology MDS09 CDS 4546831 . . . 4547733 fimH b4320 minor fimbrial subunit, D-mannose specific adhesin MDS10 CDS complement(3108612 . . . 3109148) yghD b2968 putative secretion pathway protein MDS10 CDS complement(3109150 . . . 3110010) yghE b2969 putative general secretion pathway for protein export (GSP) MDS10 CDS complement(3110076 . . . 3110942) b2970 putative general secretion pathway for protein export (GSP) MDS10 CDS complement(3111089 . . . 3111499) b2971 orf, hypothetical protein MDS10 CDS complement(3111565 . . . 3112497) b2972 MDS10 CDS complement(3117619 . . . 3119301) yghK b2975 putative permease MDS10 CDS complement(3119656 . . . 3121827) glcB b2976 malate synthase G MDS10 CDS complement(3121849 . . . 3122253) glcG b2977 orf, hypothetical protein MDS10 CDS complement(3124544 . . . 3126043) glcD b2979 glycolate oxidase subunit D MDS10 CDS 3126294 . . . 3127058 glcC b2980 transcriptional activator for glc operon MDS10 CDS complement(3127065 . . . 3128237) b2981 orf, hypothetical protein MDS10 CDS 3128200 . . . 3129216 trs5_9 b2982 IS5 transposase MDS10 CDS complement(3129363 . . . 3130430) yghQ b2983 orf, hypothetical protein MDS10 CDS complement(3130476 . . . 3131234) yghR b2984 orf, hypothetical protein MDS10 CDS complement(3131266 . . . 3131979) yghS b2985 orf, hypothetical protein MDS10 CDS 3132153 . . . 3132845 yghT b2986 orf, hypothetical protein MDS10 CDS complement(3132894 . . . 3134393) pitB b2987 low-affinity phosphate transport MDS10 CDS complement(3112572 . . . 3117134) yghJ b4466 putative lipoprotein MDS10 CDS complement(3122258 . . . 3123481) glcF b4467 glycolate oxidase iron-sulfur subunit MDS10 CDS complement(3123492 . . . 3124544) glcE b4468 glycolate oxidase iron-sulfur subunit MDS11 CDS complement(1196090 . . . 1196755) ymfD b1137 orf, hypothetical protein MDS11 CDS complement(1196756 . . . 1197460) ymfE b1138 orf, hypothetical protein MDS11 CDS 1197918 . . . 1198811 lit b1139 phage T4 late gene expression; at locus of e14 element MDS11 CDS complement(1198902 . . . 1200029) intE b1140 prophage e14 integrase MDS11 CDS complement(1200010 . . . 1200255) b1141 MDS11 CDS complement(1200292 . . . 1200603) ymfH b1142 MDS11 CDS 1200675 . . . 1201061 ymfI b1143 MDS11 CDS complement(1200999 . . . 1201283) ymfJ b1144 MDS11 CDS complement(1201482 . . . 1202156) b1145 MDS11 CDS 1201944 . . . 1202447 b1146 MDS11 CDS 1202479 . . . 1203048 ymfL b1147 MDS11 CDS 1203045 . . . 1203383 ymfM b1148 MDS11 CDS 1203393 . . . 1204760 ymfN b1149 MDS11 CDS 1204772 . . . 1204954 ymfR b1150 MDS11 CDS 1204954 . . . 1205427 ymfO b1151 MDS11 CDS 1205354 . . . 1206145 b1152 MDS11 CDS 1206136 . . . 1206720 b1153 MDS11 CDS 1206724 . . . 1207353 ycfK b1154 MDS11 CDS 1207355 . . . 1207768 b1155 MDS11 CDS complement(1207740 . . . 1208342) ycfA b1156 MDS11 CDS complement(1208342 . . . 1208881) b1157 MDS11 CDS 1208908 . . . 1209462 pin b1158 inversion of adjacent DNA; at locus of e14 element MDS11 CDS 1209569 . . . 1210402 mcrA b1159 restriction of DNA at 5-methylcytosine residues; at locus of e14 element MDS11 CDS complement(1210903 . . . 1211226) ycgW b1160 orf, hypothetical protein MDS11 CDS complement(1211926 . . . 1212330) ycgX b1161 orf, hypothetical protein MDS11 CDS complement(1212551 . . . 1213282) ycgE b1162 putative transcriptional regulator MDS11 CDS complement(1213487 . . . 1214698) b1163 orf, hypothetical protein MDS11 CDS 1215012 . . . 1215248 ycgZ b1164 orf, hypothetical protein MDS11 CDS 1215291 . . . 1215563 ymgA b1165 orf, hypothetical protein MDS11 CDS 1215592 . . . 1215858 ymgB b1166 orf, hypothetical protein MDS11 CDS 1215971 . . . 1216219 ymgC b1167 orf, hypothetical protein MDS11 CDS 1216509 . . . 1218074 b1168 putative proteases MDS11 CDS 1218824 . . . 1220344 b1169 putative ATP-binding component of a transport system MDS11 CDS 1220429 . . . 1221445 b1170 MDS11 CDS complement(1221528 . . . 1221863) b1171 orf, hypothetical protein MDS11 CDS complement(1221867 . . . 1222151) b1172 orf, hypothetical protein MDS12 CDS complement(564038 . . . 565201) intD b0537 prophage DLP12 integrase MDS12 CDS 565195 . . . 565755 b0538 putative sensory transduction regulator MDS12 CDS complement(565321 . . . 565584) b0539 MDS12 CDS 566056 . . . 566364 b0540 MDS12 CDS 566361 . . . 567227 tra5_2 b0541 MDS12 CDS 567333 . . . 567470 b0542 orf, hypothetical protein MDS12 CDS 567538 . . . 567870 emrE b0543 methylviologen resistance MDS12 CDS 568125 . . . 569651 ybcK b0544 orf, hypothetical protein MDS12 CDS 570116 . . . 570667 ybcL b0545 orf, hypothetical protein MDS12 CDS 570677 . . . 571474 ybcM b0546 putative ARAC-type regulatory protein MDS12 CDS 571689 . . . 572144 ybcN b0547 orf, hypothetical protein MDS12 CDS 572144 . . . 572314 ninE b0548 similar to phage 82 and lambda proteins MDS12 CDS 572307 . . . 572597 ybcO b0549 orf, hypothetical protein MDS12 CDS 572594 . . . 572956 rus b0550 endodeoxyribonuclease RUS (Holliday junction resolvase) MDS12 CDS 573179 . . . 573562 ybcQ b0551 orf, hypothetical protein MDS12 CDS complement(573960 . . . 574976) trs5_2 b0552 IS5 transposase MDS12 CDS complement(574981 . . . 576108) nmpC b0553 outer membrane porin protein; locus of qsr prophage MDS12 CDS 576621 . . . 576836 ybcR b0554 orf, hypothetical protein MDS12 CDS 576836 . . . 577333 ybcS b0555 bacteriophage lambda lysozyme homolog MDS12 CDS 577330 . . . 577791 ybcT b0556 bacteriophage lambda endopeptidase homolog MDS12 CDS complement(577823 . . . 578116) ybcU b0557 bacteriophage lambda Bor protein homolog MDS12 CDS complement(578407 . . . 578859) ybcV b0558 putative an envelop protein MDS12 CDS 579103 . . . 579309 ybcW b0559 orf, hypothetical protein MDS12 CDS 580057 . . . 580602 nohB b0560 bacteriophage DNA packaging protein MDS12 CDS 580577 . . . 581320 ybcX b0561 orf, hypothetical protein MDS12 CDS complement(581375 . . . 581959) ybcY b0562 orf, hypothetical protein MDS12 CDS 582098 . . . 582283 ylcE b0563 orf, hypothetical protein MDS12 CDS 582904 . . . 583653 appY b0564 regulatory protein affecting appA and other genes MDS12 CDS complement(583903 . . . 584856) ompT b0565 outer membrane protein 3b (a), protease VII MDS13 CDS 15445 . . . 16557 yi81_(—) b0016 IS186 hypothetical protein 1 MDS13 CDS complement(15869 . . . 16177) yi82_1 b0017 MDS13 CDS complement(16751 . . . 16960) mokC b0018 regulatory peptide whose translation enables hokC (gef) expression MDS13 CDS 17489 . . . 18655 nhaA b0019 Na+/H antiporter, pH dependent MDS13 CDS 18715 . . . 19620 nhaR b0020 transcriptional activator of nhaA MDS13 CDS complement(19811 . . . 20314) insB_1 b0021 IS1 protein InsB MDS13 CDS complement(20233 . . . 20508) insA b0022 IS1 protein InsA_1 MDS13 CDS complement(16751 . . . 16903) hokC b4412 small toxic membrane polypeptide MDS13 misc_RNA 16952 . . . 17006 sokC b4413 antisense RNA blocking mokC (orf69) and hokC (gef) translation MDS14 CDS complement(602639 . . . 603886) ybdG b0577 putative transport MDS14 CDS complement(603994 . . . 604647) nfnB b0578 oxygen-insensitive NAD(P)H nitroreductase MDS14 CDS complement(604741 . . . 605109) ybdF b0579 orf, hypothetical protein MDS14 CDS complement(605174 . . . 605422) ybdJ b0580 orf, hypothetical protein MDS14 CDS complement(605488 . . . 606606) ybdK b0581 orf, hypothetical protein MDS14 CDS 607288 . . . 608400 yi81_2 b0582 IS186 hypothetical protein MDS14 CDS 607059 . . . 607211 hokE b4415 small toxic membrane polypeptide MDS15 CDS 2507652 . . . 2508908 b2389 orf, hypothetical protein MDS15 CDS 2509023 . . . 2509349 ypeC b2390 orf, hypothetical protein MDS15 CDS complement(2509490 . . . 2510728) b2392 MDS15 CDS 2511064 . . . 2512266 nupC b2393 permease of transport system for 3 nucleosides MDS15 CDS 2512347 . . . 2513465 yi81_3 b2394 MDS15 CDS complement(2513665 . . . 2515971) yfeA b2395 orf, hypothetical protein MDS16 CDS complement(379293 . . . 380066) yaiO b0358 orf, hypothetical protein MDS16 CDS complement(380068 . . . 380511) b0359 putative transferase MDS16 CDS 380530 . . . 380940 yi21_1 b0360 IS2 hypothetical protein MDS16 CDS 380898 . . . 381803 yi22_1 b0361 IS2 hypothetical protein MDS16 CDS complement(381728 . . . 382114) b0362 orf, hypothetical protein MDS16 CDS complement(381963 . . . 383159) yaiP b0363 polysaccharide metabolism MDS16 CDS complement(383283 . . . 383693) yaiS b0364 orf, hypothetical protein MDS16 CDS 384399 . . . 385418 tauA b0365 taurine transport system periplasmic protein MDS16 CDS 385431 . . . 386198 tauB b0366 taurine ATP-binding component of a transport system MDS16 CDS 386195 . . . 387022 tauC b0367 taurine transport system permease protein MDS16 CDS 387019 . . . 387870 tauD b0368 taurine dioxygenase, 2-oxoglutarate- dependent MDS17 CDS complement(389121 . . . 389390) b0370 orf, hypothetical protein MDS17 CDS 389475 . . . 390935 yaiT b0371 orf, hypothetical protein MDS17 CDS complement(390963 . . . 391829) tra5_1 b0372 MDS17 CDS complement(391826 . . . 392134) b0373 putative flagellin structural protein MDS17 CDS 392239 . . . 393642 yaiU b0374 putative flagellin structural protein MDS17 CDS 393685 . . . 394353 yaiV b0375 orf, hypothetical protein MDS17 CDS complement(394354 . . . 395511) yaiH b0376 MDS17 CDS 395863 . . . 397083 sbmA b0377 sensitivity to microcin B17, possibly envelope protein MDS17 CDS 397096 . . . 398190 yaiW b0378 orf, hypothetical protein MDS17 CDS complement(398249 . . . 398557) yaiY b0379 orf, hypothetical protein MDS17 CDS 398685 . . . 399029 b0380 orf, hypothetical protein MDS18 CDS complement(2992959 . . . 2993114) b2856 orf, hypothetical protein MDS18 CDS complement(2993336 . . . 2993767) b2857 orf, hypothetical protein MDS18 CDS complement(2993770 . . . 2993991) b2858 orf, hypothetical protein MDS18 CDS complement(2993984 . . . 2994409) b2859 orf, hypothetical protein MDS18 CDS complement(2994394 . . . 2995299) yi22_4 b2860 IS2 hypothetical protein MDS18 CDS complement(2995257 . . . 2995622) yi21_4 b2861 IS2 hypothetical protein MDS18 CDS complement(2995711 . . . 2996010) b2862 orf, hypothetical protein MDS18 CDS complement(2996056 . . . 2996892) b2863 orf, hypothetical protein MDS19 CDS 3182802 . . . 3183152 b3042 orf, hypothetical protein MDS19 CDS 3183436 . . . 3183987 ygiL b3043 putative fimbrial-like protein MDS19 CDS 3184209 . . . 3184574 yi21_5 b3044 IS2 hypothetical protein MDS19 CDS 3184532 . . . 3185437 yi22_5 b3045 IS2 hypothetical protein MDS19 CDS 3185422 . . . 3187887 yqiG b3046 putative membrane protein MDS19 CDS 3187894 . . . 3188652 yqiH b3047 putative membrane protein MDS19 CDS 3188654 . . . 3189718 yqiI b3048 orf, hypothetical protein MDS20 CDS complement(687220 . . . 688236) trs5_3 b0656 IS5 transposase MDS21 CDS 1386912 . . . 1387919 ycjG b1325 putative muconate cycloisomerase I (EC 5.5 MDS21 CDS complement(1387894 . . . 1388682) ycjI b1326 putative carboxypeptidase MDS21 CDS complement(1388957 . . . 1389889) b1327 orf, hypothetical protein MDS21 CDS 1390015 . . . 1390914 ycjZ b1328 putative transcriptional regulator LYSR-type MDS21 CDS 1391230 . . . 1392864 b1329 MDS21 CDS complement(1392915 . . . 1393946) b1330 orf, hypothetical protein MDS21 CDS 1394100 . . . 1395116 trs5_4 b1331 IS5 transposase MDS21 CDS 1395389 . . . 1395646 ynaJ b1332 orf, hypothetical protein MDS21 CDS complement(1395696 . . . 1396646) ydaA b1333 orf, hypothetical protein MDS22 CDS complement(2099919 . . . 2100935) trs5_7 b2030 MDS22 CDS complement(2100940 . . . 2101413) yefJ b2031 MDS22 CDS complement(2101415 . . . 2102533) wbbK b2032 putative glucose transferase MDS22 CDS complement(2102518 . . . 2103108) wbbJ b2033 putative O-acetyl transferase MDS22 CDS complement(2103089 . . . 2104081) wbbI b2034 putative Galf transferase MDS22 CDS complement(2104084 . . . 2105250) wbbH b2035 O-antigen polymerase MDS22 CDS complement(2105250 . . . 2106353) glf b2036 UDP-galactopyranose mutase MDS22 CDS complement(2106361 . . . 2107608) rfbX b2037 putative O-antigen transporter MDS22 CDS complement(2107605 . . . 2108162) rfbC b2038 dTDP-6-deoxy-D-glucose-3,5 epimerase MDS22 CDS complement(2108162 . . . 2109043) rfbA b2039 glucose-1-phosphate thymidylyltransferase MDS22 CDS complement(2109101 . . . 2110000) rfbD b2040 dTDP-6-deoxy-L-mannose- dehydrogenase MDS22 CDS complement(2110000 . . . 2111085) rfbB b2041 dTDP-glucose 4,6 dehydratase MDS22 CDS complement(2111458 . . . 2112351) galF b2042 homolog of Salmonella UTP--glucose- 1-P uridyltransferase, probably a UDP- gal transferase MDS22 CDS complement(2112526 . . . 2113920) wcaM b2043 orf, hypothetical protein MDS22 CDS complement(2113931 . . . 2115151) wcaL b2044 putative colanic acid biosynthesis glycosyl transferase MDS22 CDS complement(2115148 . . . 2116428) wcaK b2045 putative galactokinase (EC 2.7.1.6 MDS22 CDS complement(2116704 . . . 2118182) wzxC b2046 probable export protein MDS22 CDS complement(2118184 . . . 2119578) wcaJ b2047 putative colanic acid biosynthsis UDP- glucose lipid carrier transferase MDS22 CDS complement(2119633 . . . 2121003) cpsG b2048 phosphomannomutase MDS22 CDS complement(2121108 . . . 2122544) cpsB b2049 mannose-1-phosphate guanyltransferase MDS22 CDS complement(2122547 . . . 2123770) wcaI b2050 putative colanic biosynthesis glycosyl transferase MDS22 CDS complement(2123767 . . . 2124249) wcaH b2051 GDP-mannose mannosyl hydrolase MDS22 CDS complement(2124249 . . . 2125214) wcaG b2052 putative nucleotide di-P-sugar epimerase or dehydratase MDS22 CDS complement(2125217 . . . 2126338) gmd b2053 GDP-D-mannose dehydratase MDS22 CDS complement(2126364 . . . 2126912) wcaF b2054 putative transferase MDS22 CDS complement(2126928 . . . 2127674) wcaE b2055 putative colanic acid biosynthesis glycosyl transferase MDS22 CDS complement(2127685 . . . 2128902) wcaD b2056 putative colanic acid polymerase MDS22 CDS complement(2128877 . . . 2130094) wcaC b2057 putative glycosyl transferase MDS22 CDS complement(2130091 . . . 2130579) wcaB b2058 putative transferase MDS22 CDS complement(2130582 . . . 2131421) wcaA b2059 putative regulator MDS22 CDS complement(2131514 . . . 2133712) b2060 MDS22 CDS complement(2133679 . . . 2134122) wzb b2061 low molecular weight protein-tyrosine- phosphatase MDS22 CDS complement(2134128 . . . 2135267) wza b2062 putative polysaccharide export protein MDS23 CDS complement(2284412 . . . 2286922) yejO b2190 putative ATP-binding component of a transport system MDS23 CDS 2286927 . . . 2287049 b2191 orf, hypothetical protein MDS23 CDS complement(2287087 . . . 2288103) trs5_8 b2192 IS5 transposase MDS24 CDS 3360134 . . . 3360808 yhcA b3215 putative chaperone MDS24 CDS 3360829 . . . 3363210 yhcD b3216 putative outer membrane protein MDS24 CDS 3363207 . . . 3363686 yhcE b3217 orf, hypothetical protein MDS24 CDS complement(3363724 . . . 3364740) trs5_10 b3218 IS5 transposase MDS24 CDS 3364948 . . . 3365664 yhcF b3219 putative transcriptional regulator MDS25 CDS 3649314 . . . 3650096 yhiS b3504 orf, hypothetical protein MDS25 CDS complement(3650205 . . . 3651221) trs5_11 b3505 IS5 transposase MDS26 CDS complement(1128637 . . . 1129053) flgN b1070 protein of flagellar biosynthesis MDS26 CDS complement(1129058 . . . 1129351) flgM b1071 anti-FliA (anti-sigma) factor; also known as RflB protein MDS26 CDS complement(1129427 . . . 1130086) flgA b1072 flagellar biosynthesis; assembly of basal-body periplasmic P ring MDS26 CDS 1130241 . . . 1130657 flgB b1073 flagellar biosynthesis, cell-proximal portion of basal-body rod MDS26 CDS 1130661 . . . 1131065 flgC b1074 flagellar biosynthesis, cell-proximal portion of basal-body rod MDS26 CDS 1131077 . . . 1131772 flgD b1075 flagellar biosynthesis, initiation of hook assembly MDS26 CDS 1131797 . . . 1133005 flgE b1076 flagellar biosynthesis, hook protein MDS26 CDS 1133025 . . . 1133780 flgF b1077 flagellar biosynthesis, cell-proximal portion of basal-body rod MDS26 CDS 1133952 . . . 1134734 flgG b1078 flagellar biosynthesis, cell-distal portion of basal-body rod MDS26 CDS 1134787 . . . 1135485 flgH b1079 flagellar biosynthesis, basal-body outer-membrane L (lipopolysaccharide layer) ring protein MDS26 CDS 1135497 . . . 1136594 flgI b1080 homolog of Salmonella P-ring of flagella basal body MDS26 CDS 1136594 . . . 1137535 flgJ b1081 flagellar biosynthesis MDS26 CDS 1137601 . . . 1139244 flgK b1082 flagellar biosynthesis, hook-filament junction protein 1 MDS26 CDS 1139256 . . . 1140209 flgL b1083 flagellar biosynthesis; hook-filament junction protein MDS27 CDS complement(1960604 . . . 1960996) flhE b1878 flagellar protein MDS27 CDS complement(1960996 . . . 1963074) flhA b1879 flagellar biosynthesis; possible export of flagellar proteins MDS27 CDS complement(1963067 . . . 1964215) flhB b1880 putative part of export apparatus for flagellar proteins MDS27 CDS complement(1964417 . . . 1965061) cheZ b1881 chemotactic response; CheY protein phophatase; antagonist of CheY as switch regulator MDS27 CDS complement(1965072 . . . 1965461) cheY b1882 chemotaxis regulator transmits chemoreceptor signals to flagelllar motor components MDS27 CDS complement(1965476 . . . 1966525) cheB b1883 response regulator for chemotaxis (cheA sensor); protein methylesterase MDS27 CDS complement(1966528 . . . 1967388) cheR b1884 response regulator for chemotaxis protein glutamate methyltransferase MDS27 CDS complement(1967407 . . . 1969008) tap b1885 methyl-accepting chemotaxis protein IV peptide sensor receptor MDS27 CDS complement(1969054 . . . 1970715) tar b1886 methyl-accepting chemotaxis protein II aspartate sensor receptor MDS27 CDS complement(1970860 . . . 1971363) cheW b1887 positive regulator of CheA protein activity MDS27 CDS complement(1971384 . . . 1973348) cheA b1888 sensory transducer kinase between chemo-signal receptors and CheB and CheY MDS27 CDS complement(1973353 . . . 1974279) motB b1889 enables flagellar motor rotation linking torque machinery to cell wall MDS27 CDS complement(1974276 . . . 1975163) motA b1890 proton conductor component of motor; no effect on switching MDS27 CDS complement(1975290 . . . 1975868) flhC b1891 regulator of flagellar biosynthesis acting on class 2 operons; transcription initiation factor MDS27 CDS complement(1975871 . . . 1976230) flhD b1892 regulator of flagellar biosynthesis acting on class 2 operons; transcription initiation factor MDS27 CDS complement(1976542 . . . 1977045) insB_5 b1893 IS1 protein InsB MDS27 CDS complement(1976964 . . . 1977239) insA_5 b1894 IS1 protein InsA MDS28 CDS complement(1995086 . . . 1995838) yecC b1917 putative ATP-binding component of a transport system MDS28 CDS complement(1995835 . . . 1996503) yecS b1918 putative transport system permease protein (former yecC) MDS28 CDS complement(1996518 . . . 1997600) yedO b1919 putative 1-aminocyclopropane-1- carboxylate deaminase MDS28 CDS complement(1997609 . . . 1998409) fliY b1920 putative periplasmic binding transport protein MDS28 CDS complement(1998497 . . . 1999084) fliZ b1921 orf, hypothetical protein MDS28 CDS complement(1999094 . . . 1999813) fliA b1922 flagellar biosynthesis; alternative sigma factor 28; regulation of flagellar operons MDS28 CDS complement(2000134 . . . 2001630) fliC b1923 flagellar biosynthesis; flagellin filament structural protein MDS28 CDS 2001896 . . . 2003302 fliD b1924 flagellar biosynthesis; filament capping protein; enables filament assembly MDS28 CDS 2003327 . . . 2003737 fliS b1925 flagellar biosynthesis; repressor of class 3a and 3b operons (RflA activity) MDS28 CDS 2003737 . . . 2004102 fliT b1926 flagellar biosynthesis; repressor of class 3a and 3b operons (RflA activity) MDS28 CDS 2004180 . . . 2005667 amyA b1927 cytoplasmic alpha-amylase MDS28 CDS complement(2005701 . . . 2006114) yedD b1928 orf, hypothetical protein MDS28 CDS 2006301 . . . 2007506 yedE b1929 putative transport system permease protein MDS28 CDS 2007503 . . . 2007736 yedF b1930 orf, hypothetical protein MDS28 CDS 2007845 . . . 2008513 yedK b1931 orf, hypothetical protein MDS28 CDS 2008624 . . . 2009103 yedL b1932 orf, hypothetical protein MDS28 CDS complement(2009372 . . . 2009563) b1933 orf, hypothetical protein MDS28 CDS complement(2009573 . . . 2009893) yedN b1934 orf, hypothetical protein MDS28 CDS complement(2010025 . . . 2010375) yedM b1935 orf, hypothetical protein MDS28 CDS 2010526 . . . 2010804 b1936 orf, hypothetical protein MDS28 CDS complement(2010724 . . . 2011038) fliE b1937 flagellar biosynthesis; basal-body component, possibly at (MS-ring)-rod junction MDS28 CDS 2011253 . . . 2012911 fliF b1938 flagellar biosynthesis; basal-body MS(membrane and supramembrane)- ring and collar protein MDS28 CDS 2012904 . . . 2013899 fliG b1939 flagellar biosynthesis, component of motor switching and energizing, enabling rotation and determinin its direction MDS28 CDS 2013871 . . . 2014578 fliH b1940 flagellar biosynthesis; export of flagellar proteins MDS28 CDS 2014578 . . . 2015951 fliI b1941 flagellum-specific ATP synthase MDS28 CDS 2015970 . . . 2016413 fliJ b1942 flagellar fliJ protein MDS28 CDS 2016410 . . . 2017537 fliK b1943 flagellar hook-length control protein MDS28 CDS 2017642 . . . 2018106 fliL b1944 flagellar biosynthesis MDS28 CDS 2018111 . . . 2019115 fliM b1945 flagellar biosynthesis, component of motor switch and energizing, enabling rotation and determining its direction MDS28 CDS 2019112 . . . 2019525 fliN b1946 flagellar biosynthesis, component of motor switch and energizing, enabling rotation and determining its direction MDS28 CDS 2019588 . . . 2019893 fliO b1947 flagellar biosynthesis MDS28 CDS 2019893 . . . 2020630 fliP b1948 flagellar biosynthesis MDS28 CDS 2020640 . . . 2020909 fliQ b1949 flagellar biosynthesis MDS28 CDS 2020917 . . . 2021702 fliR b1950 flagellar biosynthesis MDS29 CDS 4552599 . . . 4553372 uxuR b4324 regulator for uxu operon MDS29 CDS complement(4553513 . . . 4554343) yjiC b4325 orf, hypothetical protein MDS29 CDS 4555007 . . . 4555408 yjiD b4326 orf, hypothetical protein MDS29 CDS complement(4555401 . . . 4556312) yjiE b4327 putative transcriptional regulator LYSR-type MDS29 CDS complement(4556377 . . . 4557549) iadA b4328 isoaspartyl dipeptidase MDS29 CDS complement(4557562 . . . 4558023) yjiG b4329 orf, hypothetical protein MDS29 CDS complement(4558020 . . . 4558715) yjiH b4330 orf, hypothetical protein MDS29 CDS 4558851 . . . 4559507 yjiI b4331 orf, hypothetical protein MDS29 CDS complement(4559520 . . . 4560698) yjiJ b4332 putative transport protein MDS29 CDS complement(4560766 . . . 4561737) yjiK b4333 orf, hypothetical protein MDS29 CDS complement(4561945 . . . 4562718) yjiL b4334 putative enzyme MDS29 CDS complement(4562722 . . . 4563894) yjiM b4335 orf, hypothetical protein MDS29 CDS complement(4563989 . . . 4565269) yjiN b4336 orf, hypothetical protein MDS29 CDS complement(4565310 . . . 4566542) yjiO b4337 putative transport protein MDS29 CDS 4567021 . . . 4567332 yjiP b4338 orf, hypothetical protein MDS29 CDS 4567381 . . . 4567941 yjiQ b4339 orf, hypothetical protein MDS29 CDS complement(4568185 . . . 4569597) yjiR b4340 putative regulator MDS29 CDS 4569774 . . . 4569938 yjiS b4341 orf, hypothetical protein MDS29 CDS 4570389 . . . 4571954 yjiT b4342 orf, hypothetical protein MDS29 CDS complement(4574935 . . . 4575981) mcrC b4345 component of McrBC 5-methylcytosine restriction system MDS29 CDS complement(4575981 . . . 4577378) mcrB b4346 component of McrBC 5-methylcytosine restriction system MDS29 CDS complement(4577522 . . . 4577920) yjiW b4347 orf, hypothetical protein MDS29 CDS complement(4578091 . . . 4579485) hsdS b4348 specificity determinant for hsdM and hsdR MDS29 CDS complement(4579482 . . . 4581071) hsdM b4349 host modification; DNA methylase M MDS29 CDS complement(4581272 . . . 4584838) hsdR b4350 host restriction; endonuclease R MDS29 CDS 4584972 . . . 4585886 mrr b4351 restriction of methylated adenine MDS29 CDS complement(4585932 . . . 4586786) yjiA b4352 orf, hypothetical protein MDS29 CDS complement(4586899 . . . 4587102) yjiX b4353 orf, hypothetical protein MDS29 CDS complement(4587152 . . . 4589317) yjiY b4354 putative carbon starvation protein MDS29 CDS 4589680 . . . 4591335 tsr b4355 methyl-accepting chemotaxis protein I serine sensor receptor MDS29 CDS complement(4591384 . . . 4592745) yjiZ b4356 putative transport protein, cryptic orf, joins former yjiZ and yjjL MDS29 CDS complement(4592960 . . . 4593874) yjjM b4357 orf, hypothetical protein MDS29 CDS 4593998 . . . 4595035 yjjN b4358 putative oxidoreductase MDS29 CDS 4572158 . . . 4574878 yjiV b4486 conserved hypothetical protein MDS30 CDS 522485 . . . 526765 rhsD b0497 rhsD protein in rhs element MDS30 CDS 526805 . . . 527173 b0498 orf, hypothetical protein MDS30 CDS 527173 . . . 527883 b0499 orf, hypothetical protein MDS30 CDS 527864 . . . 528124 ybbD b0500 orf, hypothetical protein MDS30 CDS 528163 . . . 528354 b0501 orf, hypothetical protein MDS30 CDS complement(528869 . . . 529276) b0502 orf, hypothetical protein MDS31 CDS 728806 . . . 732999 rhsC b0700 MDS31 CDS 732593 . . . 732814 b0701 MDS31 CDS 732999 . . . 733325 ybfB b0702 orf, hypothetical protein MDS31 CDS 733443 . . . 734876 b0703 orf, hypothetical protein MDS31 CDS 734873 . . . 735442 ybfC b0704 orf, hypothetical protein MDS31 CDS 736327 . . . 737184 ybfL b0705 putative receptor protein MDS31 CDS 737315 . . . 738076 ybfD b0706 putative DNA ligase MDS32 CDS 1525914 . . . 1527962 rhsE b1456 MDS32 CDS 1527946 . . . 1528428 ydcD b1457 orf, hypothetical protein MDS32 CDS 1528610 . . . 1529356 b1458 orf, hypothetical protein MDS32 CDS 1529400 . . . 1529600 b1459 orf, hypothetical protein MDS32 CDS 1529840 . . . 1530976 ydcC b1460 H repeat-associated protein (ORF-H) MDS32 CDS 1531076 . . . 1531309 ydcE b1461 orf, hypothetical protein MDS32 CDS complement(1531306 . . . 1531923) b1462 orf, hypothetical protein MDS33 CDS 3616611 . . . 3617012 yhhG b3481 MDS33 CDS 3617215 . . . 3621450 rhsB b3482 rhsB protein in rhs element MDS33 CDS 3621437 . . . 3621805 yhhH b3483 orf, hypothetical protein MDS33 CDS 3622401 . . . 3623537 yhhI b3484 putative receptor MDS34 CDS complement(3759370 . . . 3759978) yibF b3592 putative S-transferase MDS34 CDS 3760206 . . . 3764339 rhsA b3593 rhsA protein in rhs element MDS34 CDS 3764360 . . . 3765202 yibA b3594 orf, hypothetical protein MDS34 CDS 3765244 . . . 3765945 yibJ b3595 orf, hypothetical protein MDS34 CDS 3766200 . . . 3766661 yibG b3596 orf, hypothetical protein MDS35 CDS complement(1041253 . . . 1043433) yccC b0981 orf, hypothetical protein MDS35 CDS complement(1043453 . . . 1043911) yccY b0982 yccY putative phosphatase MDS35 CDS complement(1043887 . . . 1045026) yccZ b0983 putative function in exopolysaccharide production MDS35 CDS complement(1045072 . . . 1047168) ymcA b0984 orf, hypothetical protein MDS35 CDS complement(1047168 . . . 1047914) ymcB b0985 orf, hypothetical protein MDS35 CDS complement(1047911 . . . 1048555) ymcC b0986 putative regulator MDS35 CDS complement(1048662 . . . 1048985) ymcD b0987 orf, hypothetical protein MDS35 CDS 1049250 . . . 1049753 insB_4 b0988 IS1 protein InsB MDS36 CDS complement(1085329 . . . 1085742) ycdP b1021 orf, hypothetical protein MDS36 CDS complement(1085744 . . . 1087069) ycdQ b1022 orf, hypothetical protein MDS36 CDS complement(1087062 . . . 1089080) ycdR b1023 orf, hypothetical protein MDS36 CDS complement(1089089 . . . 1091512) ycdS b1024 putative outer membrane protein MDS36 CDS 1092099 . . . 1093457 ycdT b1025 orf, hypothetical protein MDS36 CDS complement(1093498 . . . 1094364) tra5_3 b1026 MDS36 CDS complement(1094361 . . . 1094669) b1027 MDS36 CDS 1094746 . . . 1095069 b1028 orf, hypothetical protein MDS36 CDS 1095066 . . . 1096052 ycdU b1029 orf, hypothetical protein MDS37 CDS 2163174 . . . 2163545 b2080 orf, hypothetical protein MDS37 CDS 2163692 . . . 2165053 yegQ b2081 orf, hypothetical protein MDS37 CDS complement(2165326 . . . 2165544) ogrK b2082 prophage P2 ogr protein MDS37 CDS complement(2165626 . . . 2165772) b2083 orf, hypothetical protein MDS37 CDS complement(2165759 . . . 2166025) b2084 orf, hypothetical protein MDS37 CDS complement(2166013 . . . 2166390) yegR b2085 orf, hypothetical protein MDS37 CDS 2166736 . . . 2167635 b2086 orf, hypothetical protein MDS37 CDS complement(2167717 . . . 2168163) gatR_1 b2087 split galactitol utilization operon repressor MDS37 CDS 2168251 . . . 2168559 b2088 MDS37 CDS 2168556 . . . 2169422 tra5_4 b2089 MDS37 CDS complement(2169419 . . . 2169757) gatR_2 b2090 MDS37 CDS complement(2169857 . . . 2170897) gatD b2091 galactitol-1-phosphate dehydrogenase MDS37 CDS complement(2170945 . . . 2172300) gatC b2092 PTS system galactitol-specific enzyme IIC MDS37 CDS complement(2172304 . . . 2172588) gatB b2093 galactitol-specific enzyme IIB of phosphotransferase system MDS37 CDS complement(2172619 . . . 2173071) gatA b2094 galactitol-specific enzyme IIA of phosphotransferase system MDS37 CDS complement(2173081 . . . 2174343) gatZ b2095 putative tagatose 6-phosphate kinase 1 MDS37 CDS complement(2174372 . . . 2175232) gatY b2096 tagatose-bisphosphate aldolase 1 MDS37 misc_RNA 2165136 . . . 2165221 ryeE b4438 MDS38 CDS complement(3577791 . . . 3578828) yhhX b3440 putative regulator MDS38 CDS 3579161 . . . 3579649 yhhY b3441 orf, hypothetical protein MDS38 CDS 3579886 . . . 3581064 yhhZ b3442 orf, hypothetical protein MDS38 CDS 3581061 . . . 3581477 yrhA b3443 orf, hypothetical protein MDS38 CDS 3581506 . . . 3581781 insA_6 b3444 IS1 protein InsA MDS38 CDS 3581700 . . . 3582203 insB_6 b3445 IS1 protein InsB MDS38 misc_RNA complement(3578946 . . . 3579039) ryhB b4451 regulatory RNA mediating Fur regulon MDS39 CDS 3718072 . . . 3718284 cspA b3556 cold shock protein 7.4, transcriptional activator of hns MDS39 CDS 3718703 . . . 3719224 yi5A b3557 IS150 hypothetical protein MDS39 CDS 3719221 . . . 3720072 t150 b3558 IS150 putative transposase MDS39 CDS complement(3718471 . . . 3718623) hokA b4455 small toxic membrane polypeptide MDS40 CDS 1869885 . . . 1871555 yeaJ b1786 orf, hypothetical protein MDS41 CDS 167484 . . . 169727 fhuA b0150 outer membrane protein receptor for ferrichrome, colicin M, and phages T1, T5, and phi80 MDS41 CDS 169778 . . . 170575 fhuC b0151 ATP-binding component of hydroxymate-dependent iron transport MDS41 CDS 170575 . . . 171465 fhuD b0152 hydroxamate-dependent iron uptake cytoplasmic membrane component MDS41 CDS 171462 . . . 173444 fhuB b0153 hydroxamate-dependent iron uptake cytoplasmic membrane component

TABLE 3 Strain Type Coordinates Name B Function MDS43 CDS 331595 . . . 332683 yahA b0315 orf, hypothetical protein MDS43 CDS complement(332725 . . . 333657) yahB b0316 putative transcriptional regulator LYSR-type MDS43 CDS complement(333749 . . . 334246) yahC b0317 orf, hypothetical protein MDS43 CDS 334504 . . . 335109 yahD b0318 putative transcription factor MDS43 CDS 335149 . . . 336012 yahE b0319 orf, hypothetical protein MDS43 CDS 336002 . . . 337549 yahF b0320 putative oxidoreductase subunit MDS43 CDS 337549 . . . 338967 yahG b0321 orf, hypothetical protein MDS43 CDS 339389 . . . 340339 yahI b0323 putative kinase (EC 2.7.2.2 MDS43 CDS 340349 . . . 341731 yahJ b0324 putative deaminase MDS43 CDS 342108 . . . 343157 yahK b0325 putative oxidoreductase MDS43 CDS 343400 . . . 344215 yahL b0326 orf, hypothetical protein MDS43 CDS 344598 . . . 344873 yahM b0327 orf, hypothetical protein MDS43 CDS complement(344890 . . . 345561) yahN b0328 putative cytochrome subunit of dehydrogenase MDS43 CDS 345708 . . . 345983 yahO b0329 orf, hypothetical protein MDS43 CDS complement(346081 . . . 347667) prpR b0330 regulator for prp operon MDS43 CDS 347906 . . . 348796 prpB b0331 putative phosphonomutase 2 MDS43 CDS 349236 . . . 350405 prpC b0333 putative citrate synthase; propionate metabolism MDS43 CDS 350439 . . . 351890 prpD b0334 orf, hypothetical protein MDS43 CDS 351930 . . . 353816 prpE b0335 putative propionyl-CoA synthetase MDS43 CDS 354146 . . . 355405 codB b0336 cytosine permease/transport MDS43 CDS 355395 . . . 356678 codA b0337 cytosine deaminase MDS43 CDS complement(357015 . . . 357914) cynR b0338 cyn operon positive regulator MDS43 CDS 358023 . . . 358682 cynT b0339 carbonic anhydrase MDS43 CDS 358713 . . . 359183 cynS b0340 cyanate aminohydrolase, cyanase MDS43 CDS 359216 . . . 360370 cynX b0341 cyanate transport MDS43 CDS complement(360473 . . . 361084) lacA b0342 thiogalactoside acetyltransferase MDS43 CDS complement(361150 . . . 362403) lacY b0343 galactoside permease (lactose permease M protein) (MFS family MDS43 CDS complement(362455 . . . 365529) lacZ b0344 beta-D-galactosidase MDS43 CDS complement(365652 . . . 366734) lacI b0345 transcriptional repressor of the lac operon MDS43 CDS complement(366811 . . . 367758) mhpR b0346 transcriptional regulator for mhp operon MDS43 CDS 367835 . . . 369499 mhpA b0347 3-(3-hydroxyphenyl)propionate hydroxylase MDS43 CDS 369501 . . . 370445 mhpB b0348 2,3-dihydroxyphenylpropionate 1,2- dioxygenase MDS43 CDS 370400 . . . 371329 mhpC b0349 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase MDS43 CDS 371333 . . . 372148 mhpD b0350 2-keto-4-pentenoate hydratase MDS43 CDS 372145 . . . 373095 mhpF b0351 acetaldehyde dehydrogenase MDS43 CDS 373092 . . . 374105 mhpE b0352 4-hydroxy-2-ketovalerate aldolase MDS43 CDS 374638 . . . 375894 mhpT b0353 2,3-dihydroxyphenylpropionate 1,2- dioxygenase MDS43 CDS 375879 . . . 376535 yaiL b0354 nucleoprotein/polynucleotide- associated enzyme

TABLE 4 Strain Type Coordinates Name B Function MDS44 CDS 2599223 . . . 2599840 hyfA b2481 hydrogenase 4 Fe—S subunit MDS44 CDS 2599840 . . . 2601858 hyfB b2482 hydrogenase 4 membrane subunit MDS44 CDS 2601869 . . . 2602816 hyfC b2483 hydrogenase 4 membrane subunit MDS44 CDS 2602833 . . . 2604272 hyfD b2484 hydrogenase 4 membrane subunit MDS44 CDS 2604284 . . . 2604934 hyfE b2485 hydrogenase 4 membrane subunit MDS44 CDS 2604939 . . . 2606519 hyfF b2486 hydrogenase 4 membrane subunit MDS44 CDS 2606509 . . . 2608176 hyfG b2487 hydrogenase 4 subunit MDS44 CDS 2608186 . . . 2608731 hyfH b2488 hydrogenase 4 Fe—S subunit MDS44 CDS 2608728 . . . 2609486 hyfI b2489 hydrogenase 4 Fe—S subunit MDS44 CDS 2609479 . . . 2609892 hyfJ b2490 hydrogenase 4 component J MDS44 CDS 2609922 . . . 2611934 hyfR b2491 putative 2-component regulator interaction with sigma 54 MDS44 CDS 2611956 . . . 2612804 focB b2492 probable formate transporter (formate channel 2)

TABLE 5 Strain Type Coordinates Name B Function MDS45 CDS 2318065 . . . 2319891 atoS b2219 sensor protein AtoS for response regulator AtoC MDS45 CDS 2319888 . . . 2321273 atoC b2220 response regulator of ato, ornithine decarboxylase antizyme (sensor ATOS) MDS45 CDS 2321469 . . . 2322131 atoD b2221 acetyl-CoA:acetoacetyl-CoA transferase alpha subunit MDS45 CDS 2322131 . . . 2322781 atoA b2222 acetyl-CoA:acetoacetyl-CoA transferase beta subunit MDS45 CDS 2322778 . . . 2324100 atoE b2223 short chain fatty acid transporter MDS45 CDS 2324131 . . . 2325315 atoB b2224 acetyl-CoA acetyltransferase MDS45 CDS complement(2325389 . . . 2326165) yfaP b2225 orf, hypothetical protein MDS45 CDS complement(2326170 . . . 2327819) yfaQ b2226 orf, hypothetical protein MDS45 CDS complement(2327820 . . . 2328305) yfaS_2 b2227 orf, hypothetical protein MDS45 pseud complement(2327820 . . . 2332424) yfaS b4500 pseudogene ogene MDS45 CDS complement(2328321 . . . 2332424) yfaS_1 b2228 putative membrane protein MDS45 CDS complement(2332358 . . . 2332981) yfaT b2229 orf, hypothetical protein MDS45 CDS complement(2332978 . . . 2334666) yfaA b2230 orf, hypothetical protein

TABLE 6 Strain Type Coordinates Name B Function MDS46 CDS 3886458 . . . 3886532 tnaL b3707 tryptophanase leader peptide MDS46 CDS 3886753 . . . 3888168 tnaA b3708 tryptophanase MDS46 CDS 3888259 . . . 3889506 tnaB b3709 low affinity tryptophan permease MDS46 CDS 3889638 . . . 3890813 yidY b3710 putative transport protein MDS46 CDS 3890788 . . . 3891747 yidZ b3711 putative transcriptional regulator LYSR-type MDS46 CDS 3891892 . . . 3892653 yieE b3712 orf, hypothetical protein MDS46 CDS 3892675 . . . 3893241 yieF b3713 orf, hypothetical protein MDS46 CDS complement(3893295 . . . 3894632) yieG b3714 putative membrane/transport protein MDS46 CDS 3894797 . . . 3895462 yieH b3715 putative phosphatase MDS46 CDS 3895529 . . . 3895996 yieI b3716 orf, hypothetical protein MDS46 CDS 3896045 . . . 3896632 yieJ b3717 orf, hypothetical protein MDS46 CDS complement(3896694 . . . 3897416) yieK b3718 pyrimidine-specific nucleoside hydrolase MDS46 CDS complement(3897431 . . . 3898627) yieL b3719 putative xylanase MDS46 CDS complement(3898627 . . . 3900243) yieC b3720 putative receptor protein MDS46 CDS complement(3900312 . . . 3901724) bglB b3721 phospho-beta-glucosidase B; cryptic MDS46 CDS complement(3901743 . . . 3903620) bglF b3722 PTS system beta-glucosides, enzyme II, cryptic MDS46 CDS complement(3903754 . . . 3904590) bglG b3723 positive regulation of bgl operon

TABLE 7 Strain Type Coordinates Name B Function MDS47 CDS complement(674793 . . . 675770) ybeQ b0644 orf, hypothetical protein MDS47 CDS 675934 . . . 676641 ybeR b0645 orf, hypothetical protein MDS47 CDS 676638 . . . 678065 ybeS b0646 putative enzyme of polynucleotide modification MDS47 CDS complement(678075 . . . 678629) ybeT b0647 orf, hypothetical protein MDS47 CDS 678731 . . . 679438 ybeU b0648 putative tRNA ligase MDS47 CDS 679435 . . . 680886 ybeV b0649 orf, hypothetical protein MDS47 CDS complement(680946 . . . 682616) hscC b0650 putative heatshock protein (Hsp70 family)

TABLE 8 Strain Type Coordinates Name B Function MDS48 CDS complement(4295242 . . . 4297389) fdhF b4079 selenopolypeptide subunit of formate dehydrogenase H MDS48 CDS complement(4297587 . . . 4299053) yjcP b4080 putative enzyme MDS48 CDS complement(4299050 . . . 4301101) yjcQ b4081 putative enzyme MDS48 CDS complement(4301101 . . . 4302132) yjcR b4082 putative membrane protein MDS48 CDS complement(4302635 . . . 4304620) yjcS b4083 orf, hypothetical protein MDS48 CDS complement(4304893 . . . 4305822) alsK b4084 putative D-allose kinase MDS48 CDS complement(4305806 . . . 4306501) alsE b4085 allulose-6-phosphate 3-epimerase MDS48 CDS complement(4306512 . . . 4307492) alsE b4086 D-allose ABC transporter MDS48 CDS complement(4307471 . . . 4309003) alsA b4087 D-allose ABC transporter MDS48 CDS complement(4309130 . . . 4310065) alsB b4088 D-allose ABC transporter MDS48 CDS complement(4310124 . . . 4311014) rpiR b4089 transcriptional repressor of rpiB expression MDS48 CDS 4311373 . . . 4311822 rpiB b4090 ribose 5-phosphate isomerase B MDS48 CDS 4311891 . . . 4312220 yjdP b4487 conserved hypothetical protein MDS48 CDS complement(4312367 . . . 4313125) phnP b4092 phosphonate metabolism MDS48 CDS complement(4313127 . . . 4313561) phnO b4093 putative regulator, phn operon MDS48 CDS complement(4313548 . . . 4314105) phnN b4094 ATP-binding component of phosphonate transport MDS48 CDS complement(4314105 . . . 4315241) phnM b4095 phosphonate metabolism MDS48 CDS complement(4315238 . . . 4315918) phnL b4096 ATP-binding component of phosphonate transport MDS48 CDS complement(4316029 . . . 4316787) phnK b4097 ATP-binding component of phosphonate transport MDS48 CDS complement(4316784 . . . 4317629) phnJ b4098 phosphonate metabolism MDS48 CDS complement(4317622 . . . 4318686) phnI b4099 phosphonate metabolism MDS48 CDS complement(4318686 . . . 4319270) phnH b4100 phosphonate metabolism MDS48 CDS complement(4319267 . . . 4319719) phnG b4101 phosphonate metabolism MDS48 CDS complement(4319720 . . . 4320445) phnF b4102 putative transcriptional regulator MDS48 CDS complement(4320466 . . . 4320834) phnE_2 b4103 orf, hypothetical protein MDS48 CDS complement(4320684 . . . 4321304) phnE b4104 membrane channel protein component of Pn transporter MDS48 CDS complement(4321359 . . . 4322375) phnD b4105 periplasmic binding protein component of Pn transporter MDS48 CDS complement(4322400 . . . 4323188) phnC b4106 ATP-binding component of phosphonate transport

TABLE 9 Strain Type Coordinates Name B Function MDS49 CDS complement(764376 . . . 765098) farR b0730 transcriptional regulator of succinyl-CoA synthetase operon MDS49 CDS 765207 . . . 767183 hrsA b0731 protein modification enzyme, induction of ompC MDS49 CDS 767201 . . . 769834 ybgG b0732 putative sugar hydrolase

TABLE 10 Strain Type Coordinates Name B Function MDS50 CDS 3737728 . . . 3738981 avtA b3572 alanine-alpha-ketoisovalerate (or valine-pyruvate) transaminase, transaminase C MDS50 CDS complement(3739132 . . . 3739605) yiaI b3573 orf, hypothetical protein MDS50 CDS complement(3739707 . . . 3740555) yiaJ b3574 transcriptional repressor of the yiaK-S operon. MDS50 CDS 3740756 . . . 3741754 yiaK b3575 putative dehydrogenase MDS50 CDS 3741766 . . . 3742233 yiaL b3576 putative lipase MDS50 CDS 3742351 . . . 3742824 yiaM b3577 orf, hypothetical protein MDS50 CDS 3742827 . . . 3744104 yiaN b3578 putative membrane protein MDS50 CDS 3744117 . . . 3745103 yiaO b3579 putative solute-binding transport protein MDS50 CDS 3745107 . . . 3746603 lyxK b3580 L-xylulose kinase, cryptic MDS50 CDS 3746600 . . . 3747262 sgbH b3581 probable 3-hexulose 6- phosphate synthase MDS50 CDS 3747255 . . . 3748115 sgbU b3582 putative 3-hexulose-6- phosphate isomerase MDS50 CDS 3748109 . . . 3748804 sgbE b3583 L-ribulose-5-phosphate 4- epimerase MDS50 CDS complement(3749151 . . . 3749891) yiaT b3584 putative outer membrane protein MDS50 CDS 3750015 . . . 3750989 yiaU b3585 putative transcriptional regulator LYSR-type MDS50 CDS complement(3750986 . . . 3752122) yiaV b3586 putative membrane protein MDS50 CDS complement(3752128 . . . 3752451) yiaW b3587 orf, hypothetical protein

TABLE 11 Strain Type Coordinates Name B Function MDS51 CDS 1727111 . . . 1731727 lhr b1653 member of ATP-dependent helicase superfamily II

TABLE 12 Strain Type Coordinates Name B Function MD52 CDS complement(2858489 . . . 2859286) ygbI b2735 putative DEOR-type transcriptional regulator MD52 CDS 2859452 . . . 2860360 ygbJ b2736 putative dehydrogenase MD52 CDS 2860357 . . . 2861523 ygbK b2737 orf, hypothetical protein MD52 CDS 2861615 . . . 2862253 ygbL b2738 putative epimerase/aldolase MD52 CDS 2862258 . . . 2863034 ygbM b2739 orf, hypothetical protein MD52 CDS 2863123 . . . 2864487 ygbN b2740 putative transport protein

TABLE 13 Strain Type Coordinates Name B Function MDS53 CDS complement(2519615 . . . 2520499) xapR b2405 regulator for xapA MDS53 CDS complement(2520751 . . . 2522007) xapB b2406 xanthosine permease MDS53 CDS complement(2522067 . . . 2522900) xapA b2407 xanthosine phosphorylase

TABLE 14 Strain Type Coordinates Name B Function MDS54 CDS 4085025 . . . 4086080 yiiG b3896 orf, hypothetical protein MDS54 CDS complement(4086130 . . . 4087878) frvR b3897 putative frv operon regulatory protein MDS54 CDS complement(4087878 . . . 4088948) frvX b3898 frv operon protein M0S54 CDS complement(4088938 . . . 4090389) frvB b3899 PTS system, fructose-like enzyme IIBC component MDS54 CDS complement(4090400 . . . 4090846) frvA b3900 PTS system, fructose-specific IIA component

TABLE 15 Strain Type Coordinates Name B Function MDS55 CDS complement(1252308 . . . 1255175) yogV b1202 putative adhesion and penetration protein

TABLE 16 Strain Type Coordinates Name B Function MDS56 CDS complement(4486584 . . . 4488086) yigR b4263 orf, hypothetical protein MDS56 CDS complement(4488164 . . . 4489162) idnR b4264 L-idonate transcriptional regulator MDS56 CDS complement(4489229 . . . 4490548) idnT b4265 L-idonate transporter MDS56 CDS complement(4490610 . . . 4491374) idnO b4266 5-keto-D-gluconate 5- reductase MDS56 CDS complement(4491398 . . . 4492429) idnD b4267 L-idonate dehydrogenase MDS56 CDS 4492646 . . . 4493209 idnK b4268 gluconate kinase

TABLE 17 Strain Type Coordinates Name B Function MDS57 CDS complement(643420 . . . 644226) rna b0611 RNase I, cleaves phosphodiester bond between any two nucleotides

TABLE 18 Strain Type Coordinates Name B Function MDS58 CDS complement(2474716 . . . 2475651) dsdC b2364 D-serine dehydratase (deaminase) transcriptional activator MDS58 CDS 2475869 . . . 2477206 dsdX b2365 transport system permease (serine) MDS58 CDS 2477224 . . . 2478552 dsdA b2366 D-serine dehydratase (deaminase)

TABLE 19 Strain Type Coordinates Name B Function MDS59 CDS 879950 . . . 881152 dacC b0839 D-alanyl- D-alanine carboxypeptidase penicillin- binding protein 6

TABLE 20 Strain Type Coordinates Name B Function MDS60 CDS complement(513625 . . . 514080) ybbJ b0488 orf, hypothetical protein MDS60 CDS complement(514080 . . . 514997) ybbK b0489 putative protease MDS60 CDS 515143 . . . 515820 ybbL b0490 putative ATP-binding component of a transport system MDS60 CDS 515807 . . . 516586 ybbM b0491 putative metal resistance protein MDS60 CDS complement(516649 . . . 517503) ybbN b0492 putative thioredoxin-like protein MDS60 CDS complement(517564 . . . 518373) ybbO b0493 putative oxidoreductase MDS60 CDS complement(518363 . . . 518989) tesA b0494 acyl-CoA thioesterase I; also functions as protease I MDS60 CDS 518957 . . . 519643 ybbA b0495 putative ATP-binding component of a transport system MDS60 CDS 519640 . . . 522054 ybbP b0496 putative oxidoreductase MDS60 CDS 522485 . . . 526765 rhsD b0497 rhsD protein in rhs element MDS60 CDS 526805 . . . 527173 ybbC b0498 orf, hypothetical protein MDS60 CDS 527173 . . . 527883 ylbH b0499 orf, hypothetical protein MDS60 CDS 527864 . . . 528124 ybbD b0500 orf, hypothetical protein MDS60 CDS complement(528869 . . . 529240) ylbJ b0502 orf, hypothetical protein 

1. A strain of E. coli having a genome with the nucleic acid sequence set forth in SEQ ID NO: 1 with the following genes deleted therefrom: b0245-b0301, b0303-b0310, b1336-b1411, b4426-b4427, b2441-b2450, b2622-b2654, b2657-b2660, b4462, b1994-b2008, b4435, b3322-b3338, b2349-b2363, b1539-b1579, b4269-b4320, b2968-b2972, b2975-b2977, b2979-b2987, b4466-4468, b1137-b1172, b0537-b0565, b0016-b0022, b4412-b4413, b0577-b0582, b4415, b2389-b2390, b2392-b2395, b0358-b0368, b0370-b0380, b2856-b2863, b3042-b3048, b0656, b1325-b1333, b2030-b2062, b2190-b2192, b3215-b3219, b3504-b3505, b1070-b1083, b1878-b1894, b1917-b1950, b4324-b4342, b4345-b4358, b4486, b0497-b0502, b0700-b0706, b1456-b1462, b3481-b3484, b3592-b3596, b0981-b0988, b1021-b1029, b2080-b2096, b4438, b3440-b3445, b4451, b3556-b3558, b4455, b1786, b0150-b0153, b2945, b2699, b0315-b0321, b0323-b0331, and b0333-b0354.
 2. A method of producing a polypeptide comprising: (a) providing the strain of claim 1; (b) transforming the strain with a heterologous nucleic acid encoding the polypeptide operatively linked to an expression control sequence; and (c) culturing the transformed strain under conditions suitable to express the polypeptide.
 3. A method of introducing a heterologous nucleic acid into a microorganism comprising: (a) providing the strain of claim 1; and (b) transforming the strain with the heterologous nucleic acid.
 4. The method of claim 3 wherein the heterologous nucleic acid is a vector.
 5. A method of amplifying a heterologous nucleic acid comprising: (a) providing the strain of claim 1; (b) transforming the strain with a vector comprising the heterologous nucleic acid; and (c) culturing the transformed strain under conditions suitable to amplify amplifying the heterologous nucleic acid. 